AsepsisandAsepticPracticesintheOperatingRoom——2
Principle #4All items introduced onto a sterile field should be opened, dispensed, and transferred by methods that maintain sterility and integrity.2,4All sterile items should be dispensed to the sterile field by methods that preserve the integrity of the items and sterile field.1 Nonsterile personnel, usually the circulating nurse, must use good judgement when dispensing sterile items onto the sterile field eit......阅读全文
Asepsis－and－Aseptic－Practices－in－the－Operating－Room
Preventing surgical site infection in the operating room is the primary goal of the surgical team, and all activities performed by the team support th
Asepsis－and－Aseptic－Practices－in－the－Operating－Room——2
Principle #4All items introduced onto a sterile field should be opened, dispensed, and transferred by methods that maintain sterility and integrity.2,
基本无菌化技术
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
Principles－of－Aseptic－Technique
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
Yale－University－Policy－for－Aseptic－Amphibian－Survival－Surgery
Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the Guid
Experimental－Surgery
General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa. Experimental surgery has
Aseptic－Techniques
Aseptic techniques ensure that all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi, mycoplasm
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Principles－of－Aseptic－Technique——4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Principles－of－Aseptic－Technique——2
PersonnelAseptic technique requires careful attention to a series of steps which begins with patient and instrument preparation and ends at final woun
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
Aseptic－Technique－and－Good－Cell－Culture－Practice
AimTo ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross con
Standard－Operating－Procedures－for－T1Phage－Testing－Assay
I. Introduction:This assay uses a lawn of phage-susceptible E. coli (DH10B) embedded in a layer of agarose. This top agarose lays on a bed of standard
Dorsal－Lip－Transplant－in－Amphibian－Embryos
IntroductionIn early stages of development in amphibian embryos, very few cells' fates are rigidly determined. However, previous experiments show
Isolation－and－Quantification－of－Genomic－DNA－from－Mycobacterium－tuberculosis
Part A. Isolation of Nucleic AcidsNOTE: CAUTION! STEPS 1-10 SHOULD BE PERFORMED USING APPROPRIATE PROCEDURES FOR HANDLING MATERIAL POTENTIALLY CONTAMI
Yale－Immunofluorescence－Protocol
实验概要We provide a protocol for fixation, immunostaining, and imaging in 384-well Plates.主要试剂Reagents1. 384-well view plates (Aurora)2. HUVEC (pooled, L
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic
ELISPOT－(Enzymelinked－ImmunoSPOT)－实验方法步骤2
Cytokine ELISPOT ProtocolDescriptioneBioscience ELISPOT Ready-SET-Go! reagent sets contain the necessary reagents for performing enzyme linked immunos
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
实验概要 Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspect
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose:A: Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected
使用量子级联激光器的可调谐长波红外陷波滤波器的...1
使用量子级联激光器的可调谐长波红外陷波滤波器的性能特征我们描述了在可调谐整个光谱范围内使用量子级联激光器（QCL）在8至12微米的长波红外中操作的可光谱调谐微工程陷波滤波器的设计和性能表征。滤波器的设计基于导模共振现象。器件结构由平面波导顶部的亚波长介电光栅组成，该光栅使用高折射率透明介电材料，即折
LIVE/DEAD®－Violet－Viability/Vitality－Kit
实验概要The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneo
基本无菌化技术3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
基本无菌化技术4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Axolotl－Protocol:－Transplantation－of－the－Gill－Organ－Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
ELISPOT－(Enzymelinked－ImmunoSPOT)
IntroductionEnzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, bu
酶制剂在乳品辅料中应用方法介绍
INDUSTRY GUIDELINES FOR THE USE OF ENZYMES IN DIETARY SUPPLEMENTSThese Guidelines have been prepared by the Enzyme Technical Association (ETA) to assi
LIVE/DEAD®－Violet－Viability/Vitality－Kit
实验概要The LIVE/DEAD® Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultane
SOLID－TUMOR－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose:A. Samples of solid tumors or lymph nodes may be sent from patients with cancer. These samples should be processed directly and also set up