ConstructionandManipulationofLargeInsertBacterialCloneLibraries
Acknowledgements The organizer of the workshop acknowledges Dr. Murray Milford, Professor and Interim Head, and Dr. Mark Hussey, Professor and Interim Associate Head, Department of Soil and Crop Sciences, Texas A&M University for their valuable suggestions and support, and Ms. Chantel Scheuring and Ms. Elizabath Huff for their assistance in organizing the workshop. The author of the manual thanks Ms. Chantel......阅读全文
M13噬菌体
· M13 Phage (Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phag
Basic－procedures－for－bacteria－culture2
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo
codon－usage－database
AnnouncementCodon Usage Database is an extended WWW version of CUTG (Codon Usage Tabulated from GenBank). The frequency of codon use in each organism
人工转录因子的部件——人类锌指结构2
Table 2: Binding sites and identity of ZFPs used in VEGF activationWe then generated artificial transcription factors by fusing the three-finger domai
Expression－L19－using－Pichia－pastoris
Pichia pastoris is a methylotropic yeast used to express high amounts of protein. Secreted expression is achieved with a cleavalable faktor. To yield
直播预告|宋延林研究员讲述纳米绿色印刷制造技术
原文地址：http://news.sciencenet.cn/htmlnews/2024/3/519146.shtm 直播时间：2024年3月15日（周五）20:00-21:30 直播平
质粒
Extrachromosomal Elements-Plasmids (Michael Blaber)What's plasmid? Find answer here. It provides background information on the features of plasmid
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（二）
Figure 2: Verification of C1 Single-Cell mRNA-Seq data quality. a)ERCC RNA Spike-In Control Mix 1 was applied to a C1 IFC at a total transcript in
胚胎干细胞培养
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routine Splitting and freezing of cells (
基于epMotion－5075t及KAPA文库定量试剂盒的全自动NGS文库...2
Results and DiscussionAutomated qPCR Setup Accuracy and PrecisionThe accuracy and precision of the qPCR setup was evalu ated based on standard curve
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（一）
Introduction Multi-cellular populations are fundamentally driven by the collective properties of individual cells. However, our understanding of ge
Cas9CKI小鼠繁育原则与路线讲解
随着基因工程技术的发展，针对特定基因研究的小鼠模型构建也越来越成熟。但是，构建成功的模型小鼠要用于实验，还需要漫长的繁育之路要走。行百里者半九十，你以为模型构建成功了就结束了吗？NO NO NO！繁育是提供合格实验材料过程中的临门一脚。特别是对于CKO、CKI这些组织特异性的模型小鼠，需要与cre工
酵母人工染色体
· Easy YAC Preparation Method (Andrew Davies,Shaw lab)· Screening YAC libraries (Donis Keller Lab)This is a method for screening YAC l
The－TRC－shRNA设计方法与原则
OverviewWe design shRNA molecules with an algorithm. Our algorithm uses several criteria to rank potential 21mer targets within each human and mouse R
如何借助epMotion－5073l移液工作站完成微量体积的...（二）
Figure 1: epMotion 5073l worktable for qPCR setup Results and DiscussionThe automation of the qPCR setup using the 10 µL dispensing tool and the epM
基于epMotion－5075t及KAPA文库定量试剂盒的全自动NGS文库...1
基于epMotion 5075t及KAPA文库定量试剂盒的全自动NGS文库定量操作Automated KAPA® Library Quantifcation Kit with the epMotion® 5075tMaud Brasseur¹, Jennifer Pavlica², Marsha M
Immunodetection－of－cyclin－D1－and－D2/D3－using－flow－cytometry
DescriptionThis protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser ex
超级感受态细胞的制备
The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" CellsJoseph SambrookPeter Maccallum Cancer Institute and T
PCR－Primer－Design（一）
Molecular Biology Today 2001. 2(2): 27-32. Vinay K. Singh and Anil Kumar Bioinformatics Sub-centr
Targeted－Gene－Replacement－in－Fungal－Pathogens－via－Agrobacterium－...
Genome sequence data on fungal pathogens provide the opportunity to carry out a reverse genetics approach to uncover gene function. Efficient meth
Platensilin、Platensimycin、Platencin及其类似物的不对称合成和生物学评价
山东大学娄红祥团队报道了基于生物启发骨架重建方法的Platensilin, Platensimycin, Platencin及其类似物的不对称合成和生物学评价。相关研究成果于2024年7月8日发表在国际知名学术期刊《美国化学会志》。Platensilin、platensimycin和platncin
克隆的概念和技术特点
克隆是指生物体通过体细胞进行的无性繁殖，以及由无性繁殖形成的基因型完全相同的后代个体。通常是利用生物技术由无性生殖产生与原个体有完全相同基因的个体或种群。克隆是英文"clone"或"cloning"的音译，而英文"clone"则起源于希腊文"Klone"，原意是指以幼苗或嫩枝插条，以无性繁殖或营养繁
2014合成生物学国际会议日程公布
一、会议内容安排 报到日期：2014年10月25日，下午2:00-8:00pm 报到地点：贵州大厦 会议日期：2014年10月26-27日 会议地点：北京化工大学会议中心 住宿地点：北京化工大学招待所 樱花宾馆 如家快捷酒店 贵
454高通量测序——研究土壤微生物的新手段
在陆地生态系统中，在土壤中生活有数量庞大的微生物种群，包括原核微生物如细菌、蓝细菌、放线菌及超显微结构微生物, 以及真核生物如真菌、藻类( 蓝藻除外) 、地衣等。它们与植物和动物有着明确的分工，主要扮演“分解者”的角色，几乎参与土壤中一切生物和生物化学反应，担负着地球C、N、P、S 等物质循环的
细菌培养
Preparing Overnight Bacteria Culture (LaboratoryExperiments.com)This is a basic procedure for high school students and useful for those who are new to
克隆技术（一）
克隆是英文"clone"或"cloning"的音译，而英文"clone"则起源于希腊文"Klone"，原意是指以幼苗或嫩枝插条，以无性繁殖或营养繁殖的方式培育植物，如扦插和嫁接。很多植物都是通过克隆这样的无性生殖方式从单一植株获得大量的子代个体。在生物学上，是指选择性地复制出一段DNA序列（分子克隆
Th17－Polarization－of－Mouse－CD4－Cells
实验概要T helper 17 (Th17) cells are a subset of CD4 T helper cells characterized by their production of IL-17, particularly IL-17A and IL-17F. They are
NCBI使用方法之二：如何查找连续的mRNA、cDNA、蛋白序列
1、进入NCBI主页在search后面选择Gene，在for后面填写需要查找的基因的名字。点击“Go”，出现了很多基因序列，在每个序列的右边还有“Order cDNA clone” 的链接，这些序列中有些序列是跟你的目的基因同名的，有些是别名（Other Aliases）与你的目的基因一致，根据每个
Generating－stable－cell－lines－in－HEK293
Generating stable cell lines in HEK293Prior to transfection, it is recommended that you linearize your pcDNA gene construct. Linearizing will decrease
specific－immunodetection－of－cyclins－using－488/630－dual－laser－flow－cytometry
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometryWilliam Telford Hospital for Special SurgeryThis protocol is f