ProtocolsforLCMpreparationandanalysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Preparation and LCM of Paraffin Embedded Tissue SectionsFor both I & II Frozen & Paraffin-EmbeddedTissue SectionsD. Laser TransferE. DNA Extraction Protocol of LCM tissue and PCR for DNA analysisF. RNA Extraction Protocol of LCM ......阅读全文
DNA克隆
DNA克隆（主要内容如下）· General Procedure· PCR Cloning· Subcloning· ET Cloning· Vector Preparation· Ligation Re
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites五
RJ provides efficient energetic fuels for the fast development of larvae and the egg-laying queen through the metabolism of sugars, lipids, and pr
Human－Embryonic－Stem－(ES)－Cell－Protocols——Freezing－Human－ES－Cells
Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up).With a 5 ml pipet, gently pipet and scrape colonies f
Human－Embryonic－Stem－(ES)－Cell－Protocols——Thawing－Human－ES－cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form.Thaw cryovial by gently swirling in waterbath until only a small
德国IBL－ASP－ELISA试剂盒使用说明（四）
J. PREPARATION OF DA SAMPLES FROM OTHER MATRICES a) Preparation of algal samplesThe analysis of algal samples will depend on the amount of algae (cel
Preparation－of－High－Titer－Adenovirus－in－P11－cells
adapted from Cell Biology, A Lab Manual, second edition, volume 1 -Grow up P11 cells in 15 cm plates to 70 – 80% confluence. -Infect cells with a MOI
Cosmid－Cloning:－Cell－preparation,－DNA－packaging,－and－Cell－Transfection
Cosmid Cloning: Cell preparation, DNA packaging, and Cell TransfectionProtocol taken from Stratagene's Gigapack packaging extracts instruction man
Preparation－of－cytoplasmic－extracts－for－the－application－inacellfree－system
Characteristics of this procedure:Cells are grown to 80% confluency, then harvested, washed and disrupted in KPM buffer by freezing-thawing cycles wit
A－practical－method－for－the－preparation－of－total－DNA－from－filamentous－fungi
Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chlori
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells3
Moreover, when the method was applied to the analysis of the cellular composition of immature testes, the results were also in agreement with previous
Method:－Reactivating－Cell－Lines－and－Cell－Growth－for－DNA－Preparation
Purpose:Cell lines are reactivated and grown to a count of 1 x 108 cells. The cells are pelleted and stored frozen at -80 degrees C prior to DNA extra
Method:－Preparation－of－Lymphoblastoid－Cell－Lines－for－Long－Term－Storage
Method: Preparation of Lymphoblastoid Cell Lines for Long Term StorageMay 30, 1990Rosalie VeilePurpose:To store cell lines in a form that will insure
技术前沿空间转录组测序技术的展望
对生命过程的理解不光限于对组成个体的单个细胞的研究，更需要对细胞和细胞之间怎么样的互作协调来完成一个整体的生物学功能的研究。空间组学技术的兴起，使得在保留样本空间位置信息的同时，检测细胞中的基因表达等分子信息来勾勒出空间中的细胞表达特征。在2019年《Nature Reviews Genetic
【技术前沿】空间转录组测序技术的展望
对生命过程的理解不光限于对组成个体的单个细胞的研究，更需要对细胞和细胞之间怎么样的互作协调来完成一个整体的生物学功能的研究。空间组学技术的兴起，使得在保留样本空间位置信息的同时，检测细胞中的基因表达等分子信息来勾勒出空间中的细胞表达特征。在2019年《Nature Reviews Genetic
瑞士万通推出930紧凑型智能离子色谱
New «work horse» for routine analysis – Metrohm introduces 930 Compact IC Flex ion chromatograph Metrohm launches the 930 Compact IC Flex, a compac
RNA提取
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Procedures for Handing
蛋白质电泳
蛋白质电泳（主要内容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel Gel StainingRecipesOne-Dimensional SDS-PAGE·
酵母准备
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (rapid glass bead
Twohybrid－analysis－of－genetic－regulatory－networks2
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
Lipid－analysis－Week－3:－GAS－LIQUID－CHROMATOGRAPHY2
B: GLC procedureThe GLC will be prepared for your use. Please do not attempt to turn on the GLC on your own if it is off. Do not change any of the set
江桂斌院士：Environmental－－Food－Analysis：Problem－－Challenges
2014年4月21日下午，第十届全国生物医药色谱及相关技术学术交流会大会报告在威海召开。来自中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室的江桂斌院士带来了题为《Environmental & Food Analysis：Problem & Challeng
QUALITATIVE－ANALYSIS－OF－DNA－FRAGMENTATION－BY－AGAROSE－GEL－ELECTROPHORESIS2
3. Commentary 3.1. Background informationApoptosis is an innate mechanism of eukariotic cell suicide which plays a major role in many physiological
Immunohistochemistry－Protocol－for－ParaffinEmbedded－Sections
实验概要The following is a general procedure guide for preparation and staining of formalin-fixed, paraffin-embedded tissues using a purified, unconjuga
细胞培养——细胞保藏
Working Cell Bank (Contributed by Nanci Donacki)Provides detailed protocol for establishing a working cell bank Master Cell Bank (Contributed by Na
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（一）
Introduction Multi-cellular populations are fundamentally driven by the collective properties of individual cells. However, our understanding of ge
高通量测序的实验过程
1.样本准备（sample fragmentation）2.文库构建(library preparation)3.测序反应(sequencing reaction)4.数据分析(data analysis)
高通量测序的实验过程
1.样本准备（sample fragmentation）2.文库构建(library preparation)3.测序反应(sequencing reaction)4.数据分析(data analysis)
Human－Embryonic－Stem－(ES)－Cell－Protocols——Splitting－Human－ES－cells－on－MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 °C in a water bath.Aspirate media off of cell culture plate.Add the following a
Human－Embryonic－Stem－(ES)－Cell－Protocols——General－notes－on－ES－cell－culture
hES media has a two week shelf life.hES cells should be cultured in 4, 6, 24, 48, 96 well plates. Growing cells in flasks is not recommended because i
Human－Embryonic－Stem－(ES)－Cell－Protocols—Splitting－Human－ES－cells－onMatrige
based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of co