LCMPROTOCOLS
Slide SectioningParaffin blocks- For DNA analysis:Special LCM processing schedule is followed.The water bath is cleaned using RNAse Zap™, rinsed thoroughly with triple distilled water. The water bath is filled with triple distilled water.All instruments used are cleaned with 100% EtOH (brushes, forceps, pencil).Sections not used are toweled off with kimwipes immediately.A new blade is used for eac......阅读全文
免疫荧光
Immunofluorescence Technique (Spector Lab)protocol for immunofluorescence on cells Immunofluorescence Protocol (Walter Steffen)Methanol fixationForma
使用Ostro样品制备板结合LCMS/MS对牛奶中的兽药...（二）
使用Ostro样品制备板结合LC-MS/MS对牛奶中的兽药多残留进行筛查磷脂去除牛奶中含有大量的磷脂，约占其总脂肪含量的1% 3。在LC分离中，这些磷脂会直接干扰后洗脱的化合物。此外，结果表明磷脂残留的累积会使反相LC分离的性能变差4。图3展示了采用为鉴定磷脂而选择的MRM通道（m/z184>184
PCR基本实验方法（五）
Cloning PCR ProductsT-A Cloning Strategy: Taq and other polymerases seem to have a terminal transferase activity which results in the non-templated ad
Blackburn:Yeast－Colony－PCR
OverviewThis is a quick and easy yeast colony PCR protocol that does not require zymolyase step.Updated Protocol: Blackburn Lab: Quick and Easy Yeast
NASA利用FluorPen进行空间生物实验
美国国家航空航天局（NASA）新一代先进植物培养器（Advanced Plant Habitat，APH）搭载联盟号MS-04货运飞船抵达国际空间站，按计划展开植物生理学及太空食物种植（ growth of fresh food in space）的研究。 NASA肯尼迪航天中心利用探头式Fluor
免疫组织化学
· Double Peroxidase (HRP) Immunohistochemical Labeling of Trypsin-Sensitive Antigens (KPL)· Immunohistochemistry (Tyner lab)This is a
细胞培养——细胞保藏
Working Cell Bank (Contributed by Nanci Donacki)Provides detailed protocol for establishing a working cell bank Master Cell Bank (Contributed by Na
印刷光子晶体生物检测芯片研究获进展
随着医疗卫生水平提升，具有小型、快速、便捷等特点的即时检测（POCT）方法备受关注。为满足POCT应用需求，有研究设计出多种新型光学生物传感器，并在单个生物芯片上检测出生物标志物。近年来，光子晶体生物传感器凭借高灵敏度、高选择性、快速响应、易于集成、低成本等优势，有望成为生物医学检测领域的新方法。中
PCR基本实验方法（五）
Cloning PCR ProductsT-A Cloning Strategy: Taq and other polymerases seem to have a terminal transferase activity which results in the non-templated ad
一例紫杉醇致急性左心衰病例分析
临床资料患者，女，65岁，因“宫颈低分化鳞癌Ⅲb期化疗后4月，阴道出血4天”于2011年5月6日入院。既往使用“吉西他滨+卡铂”化疗两周期，均顺利完成。有糖尿病5年余。人院完善检查后于5月18日11:00行双侧髂内动脉造及化疗栓塞术，方案:紫杉醇210mg + 卡铂 300mg，明胶海绵 0.lcm
氮氧化物分析仪的日常保养
提到氮氧化物分析仪，熟悉它的人肯定不会不知道它的巨大作用。可以说在社会的各行各业的发展中都能看到氮氧化物分析仪的身影。所以它的地位和作用不容忽略，所以对于它的日常维修工作更是不能马虎，那么氮氧化物分析仪的日常保养常识具体有哪些呢： (一)当日保养 1．每天做完样品后作一次去蛋白维护可
重金属检测仪的检测方案
1 适用范围 用于果蔬中游离铅及水中铅含量的定性或半定量检测。 2 检测原理 样品经处理后铅与反应试剂显色，与果蔬铅含量快速检测色阶卡进行比较，即可读出被测样品中铅含量的参考浓度。 3 主要仪器 剪刀、电子秤、塑料试管。 4 试剂 ①试剂A浓酸溶液。 ②试剂B O．2mol/L三
食品中重金属检测仪的检测方案
1 适用范围 用于果蔬中游离铅及水中铅含量的定性或半定量检测。 2 检测原理 样品经处理后铅与反应试剂显色，与果蔬铅含量快速检测色阶卡进行比较，即可读出被测样品中铅含量的参考浓度。 3 主要仪器 剪刀、电子秤、塑料试管。 4 试剂 ①试剂A浓酸溶液。 ②试剂B O．2mol/L三
氮氧化物分析仪日常保养与维护
提到氮氧化物分析仪，熟悉它的人肯定不会不知道它的巨大作用。可以说在社会的各行各业的发展中都能看到氮氧化物分析仪的身影。所以它的地位和作用不容忽略，所以对于它的日常维修工作更是不能马虎，那么氮氧化物分析仪的日常保养常识具体有哪些呢： (一)当日保养 1．每天做完样品后作一次去蛋白维护可大大
常染色体显性遗传寻常鱼鳞病的介绍
常染色体显性遗传性寻常鱼鳞病，本型为常见的轻型鱼鳞病，亲代一方或双方患病则家中常有多人摧患，但无性别差异。常自幼年发病，随年龄增长而加剧，至青春期症状最为显著，以后即停止发展。皮损表现轻重不一，轻者仅冬季皮肤干燥，无明显鳞屑，搔抓后有粉状落屑，称为干皮症。或于毛囊口见有针尖大小与毛囊与致的褐色或
RNA－FISH－on－cultured－cells－in－interphase
IntroductionFluorescence in situ hybridization (FISH) has become a widely used method in genome and molecular genetic studies. The technique is highly
Lowry－–－Protein－Determination
Lowry – Protein Determination(From Protein Protocols on CD-ROM Humana Press, 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H. Wat
细胞遗传学——原位杂交(ISH)
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization, as the name suggests, is a method of localizing,
显微镜技术——电子显微技术
The Transmission Electron Microscope (TEM) (HEI)An explanation of how the TEM works. TEM Specimen Preparation (HEI) Serial Sectioning (Walter Steffe
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
粘粒
Preparation of Cosmid DNAPreparation of Cosmid DNA from 50 ml Cultures (Donis Keller Lab)Cosmid vectors containing foreign DNA inserts are known to re
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
ELISA
Gangliosides ELISA protocol (Contributed by pingsunjim)This protocol can be used for detection of gangliosides.Specific antibodies to gangliosides are
M13噬菌体
· M13 Phage (Michael Blaber)Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phag
Brain－Synaptosomal－...
实验概要The protocol provides a method of brain synaptosomal preparation.主要试剂 1. 2.55 M sucrose (unbuffered) 1) Heat on a stirring block to dissolve, t
Cellulase－assays
Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the fruit. In cases of p
Cryopreserving－Neural－Stem－Cells
实验概要There are numerous protocols available for cryopreserving neural stem cells (NSCs) derived from human embryonic stem cells; the primary objectiv
Cellulase－assays
实验概要 Cellulase enzymes show activity during the ripening of some fruits, where their effects on cell walls results in softening of the frui
Restriction－digestion
Restriction digestionRestriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restriction
学做实验的方法初步入门:迷你离心机技术文章
学做实验的方法初步入门-迷你离心机技术文章实验方法：学做实验两者方法有所不同，大概分为两种方法：向别人学习和自学。下面分别介绍一下吧基本操作和基础知识在开始学做实验的时候，最好有人指导。做实验的内容，大概分为基本操作和具体实验的操作。基本操作是具体实验的基础，例如：怎样量取容积，怎样测量和调节PH值