PreparationandStainingofParaffinSections
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and approved euthanasia techniques. Tissues to be fixed and processed should be cut to a size no larger than 3mm thick. Let tissues fix in 10% formalin at room temperature for 8 hours but not to exceed 24 hours. For small rodent tissue, it is recommended to fix tissues for 4-......阅读全文
Bromodeoxyuridine－Immunohistochemistry
Introduction: This method for the detection of cellular proliferation includes several modifications of a previously published protocol (Hayashi, et a
Complete－Mouse－Necropsy
Euthanasia Euthanasia and mouse necropsies require prior IACUC approval. The mode of euthanasia should be chosen which minimizes pain or distres
eBioscience流式抗体常见问题与解答
1、eBioscience公司提供了哪些抗体？ A：物种——人、小鼠、大鼠、非人灵长类、犬类等。检测指标——包括细胞表面标记（CD分子，膜骨架，趋化因子受体）和胞内指标（细胞因子，核内转录因子）。抗体标记——生物素标记抗体，亲和纯化抗体以及直标荧光素抗体。eB抗体的直标荧光素有（括号内是发射光波长
Multicolour－3DFISH－in－vertebrate－cells6
Back to topReviewer CommentsReviewed by: Luis Antonio Parada, CIC Biogune, Derio, Spain.In my experience the primers are better preserved when kept at
RNA提取
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Procedures for Handing
E.－Immunohistochemi...
实验概要We provide a guideline procedure and tips for staining of paraffin embedded sections, including antigen retrieval, chromogenic detection and flu
Zebrafish－whole－mou...
实验概要Whole mount staining of Zebrafish embryos, now commonly used, requires extra steps to fix and permeabilize to ensure the egg membrane is perme
DNA抽提
DNA抽提（主要内容如下）· Working with DNA· DNA Extraction from Bacteria and Other Organisms· DNA Extraction from Cell and Tissue· Mitochondria DNA Isola
反向PCR
主要内容如下：· RT-PCR· Competitive and Quantative RT-PCR· In Situ RT-PCR· RL-PCR· DNA Contamination· RT-PCR
Immunofluorescence－Microscopy－Protocol
实验概要 Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically whic
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Multicolour－3DFISH－in－vertebrate－cells3
Pepsin treatmentEquilibrate slides (kept in 50%FA/2xSSC) in 2xSSC 2 minutes;Switch to 1xPBS 3 minutes;Pepsin: 3-5 minutes in 0.01 N HCl/0.002% pepsin.
Histopathological－Approach－to－Rat－Liver－Tissue
Procedure After deep ether anesthesia, dissect the rat’s liver (Wistar albino rats, 200 – 250 g) by cutting on the ventral side. Fix 2 – 3 mm.
immunofluorescence－of－rabbit－antimurine－RELMα－by－Peprotech
实验概要The following protocol provides a method of immunofluorescence of rabbit anti-murine RELMα by Peprotech.实验步骤The following protocol used B6 mice
Chick－or－Mouse－embr...
实验概要The following procedure describes the procedure for whole mount staining of chick or mouse embryo’s. A similar procedure could be used for sta
IHC－frozen－sections...
实验概要 The method provides a guideline procedure and tips for staining of frozen sections. 实验步骤 Frozen sections: Once mounted on APES coated slides,
Immunohistochemistr...
实验概要Immunohistochemistry is a classic technique used for the localization of antigenic target molecules in tissue. The method exploits the princi
Immunohistochemistry－using－AntiGanglioside－Antibodies
Immunohistochemistry using Anti-Ganglioside Antibodies Tadashi Tai~Head, Department of Tumor Immunology, The Tokyo Metropolitan Institute of Medi
Fluorescent－Staining－of－Cells
1. Fluorescent phalloidin in methanol. Phallacidin does not work as well. Dilute 10 ul 330 nM stock into 500 ul PBS for each large coverslip. 2.
Silver－Staining－Protocol
1x 40min - overnight 50% MeOH, 12% Acetic Acid 1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde 3x 2
Staining－Methods－for－cell
death Z. Xia 10/2/95 The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells;
Alkaline－phosphatase－staining
4.5.1.1 General informationEndothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted
Protein－Staining－Procedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
Intracellular－Staining－Protocol
1. Fix cells- Add16% formaldehyde directly into culture medium to obtain a final concentration of 1.5% formaldehyde.2. Incubate in fixative for 10 min
Wholemount－staining－of－embryos
Fix embryos in formalin or MEMFA for one hour at room temperature with mixing. Rinse with TBS, replace with methanol, store at -20oC.Rehydrate by slow
ImmunoLaser－Capture－Microdissection
A: Development of Immuno-LCMLimitation of MicrodissectionMicrodissection of routinely stained or unstained frozen sections has been used successfully
Extraction－of－RNA－from－Frozen－Sections
RNA Extraction from Frozen Tissue Sections Tissue Handling: Note that all unfixed human tissue should be handled as BioSafety Level 2 materials (wear
Apoptosis－TUNEL－Assay－(frozen－sections)
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
ALKALINE－PHOSPHATASE－(APAAP)－TECHNIQUE
Preparation: Cytological Preparations Fixation: Air dry films or cytospin preparations overnight at room temperature. For frozen and paraffin secti
Preparation－of－tubulin
Although many protocols for tubulin preparation are available, the procedure described below is the simplest and highest yielding preparation I have