InSituHybridizationtoSomaticChromosomesinDrosophila
In Situ Hybridization to Somatic Chromosomes in DrosophilaAbby F. DernburgINTRODUCTIONIn situ hybridization was originally developed as a technique for visualizing and physically mapping specific sequences on Drosophila melanogaster polytene chromosomes. Hybridization techniques can also be used to localize sequences on smaller, diploid chromosomes, such as condensed mitotic chromosomes. Variations of the m......阅读全文
Overview－of－telomerase－RNA－component－gene－hTerc－Transcriptional－Regulation
Telomerase is an enzyme which replicates the terminal sequences of eukaryotic chromosomes, namely the telomeres. Cells which have an unlimited replica
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
实验概要The Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem cells)
Immunofluorescent－Staining－of－Drosophila－Larval－Brain－Tissue
INTRODUCTIONThe Drosophila larval brain is a well-established model for investigating the role of stem cells in development. Neuroblasts (neural stem
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
Mitochondrial－DNA－Isolation－from－Somatic－Embryogenic－Cell－Cultures－of－Larix
Mitochondrial DNA is isolated by a modification of the methods described by Wilson and Chourey (1984) and Radetzky (1990). Cell cultures at four d
直接原位PCR（In－situ－PCR）
原位PCR是原位杂交细胞定位和PCR的高灵敏度相结合的技术，使得靶基因检测有了极大的改进。此技术是在细胞（爬片、甩片或涂片）或组织（石蜡、冰冻切片）上直接对靶基因片段进行扩增，通过掺入标记基团直接显色或结合原位杂交进行检测的方法。一、组织切片和细胞样品的制备试剂与配置10%福尔马林；二甲苯；PBS操
直接原位PCR（In－situ－PCR）
原位PCR是原位杂交细胞定位和PCR的高灵敏度相结合的技术，使得靶基因检测有了极大的改进。此技术是在细胞（爬片、甩片或涂片）或组织（石蜡、冰冻切片）上直接对靶基因片段进行扩增，通过掺入标记基团直接显色或结合原位杂交进行检测的方法。一、组织切片和细胞样品的制备试剂与配置10%福尔马林；二甲苯；PBS操
酵母人工染色体
· Easy YAC Preparation Method (Andrew Davies,Shaw lab)· Screening YAC libraries (Donis Keller Lab)This is a method for screening YAC l
Mapping－Protein－Distributions－on－Polytene－Chromosomes－by－Immunostaining2
12. Hold the salivary glands at the common duct with tweezers.Transfer the glands to a drop of fixing solution on a siliconizedcoverslip. 13.
杨芃原：Exploration－of－human－chromosomes－by－biomass－spectrometry
复旦大学化学系 杨芃原教授 2014年4月26日，首届全国质谱分析学术研讨会在北京西郊宾馆盛大开幕。来自复旦大学的杨芃原教授带来了题为《Exploration of human chromosomes by biomass spectrometry based
Mapping－Protein－Distributions－on－Polytene－Chromosomes－by－Immunostaining1
INTRODUCTIONThe formidable size and structure of polytene chromosomes allowmapping of chromosomal protein distributions at very high resolution.This p
原位PCR
About in situ PCR (Applied Biosystems) Basic information about in situ PCR and its applications. The In Situ PCR: Amplification and Detection in
Live－imaging－with－Drosophila－tissue－culture－cells2
Materials & ReagentsDrosophila Schneider S2 cellsSchneiders Medium (GIBCO/Invitrogen), 10% fetal calf serum, Antibiotics (Sigma A5955)Depression slide
Live－imaging－with－Drosophila－tissue－culture－cells1
IntroductionLive imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It allows follow
Detection－of－apoptotic－process－in－situ－using－immunocytochemical
1. INTRODUCTION Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological
Multicolour－3DFISH－in－vertebrate－cells6
Back to topReviewer CommentsReviewed by: Luis Antonio Parada, CIC Biogune, Derio, Spain.In my experience the primers are better preserved when kept at
DIRECT－AND－SHORTTERM－PROCEDURE－FOR－HARVESTING－BONE－MARROW－CHROMOSOMES
I. PurposeTo identify chromosome anomalies in hematopoietic cells. Used especially for chromosome studies for hematological disorders such as preleuke
细胞遗传学——原位引物启动技术(PRINS)
· Hiro Hirai's Primed in Situ Synthesis (Schistosoma Genome Network)The PRINS (Primed in situ) technique uses a specific primer, dNTPs wit
Whole－mount－TUNEL－analysis－of－Xenopus－embryos
Fixation and pretreatmentDejelly albino embryos carefully in 2% Cystein (pH 7.8).Remove the vitellin membrane with two pairs of tweezers (or c
Silver－Acetate－Autometallography－(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for
In－Situ－Cell－Death－(Apoptosis)－Detection－by－TUNEL－labeling
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Beg
DNA微序列技术
· Protocols for Making Drosophila Arrays (Stanford U.)Detailed protocol for making arrays including PCR Amplification of cDNAs for Printing,
什么是荧光原位杂交有什么用？
原位杂交(In Situ Hybridization)也叫原位杂交组化(in situ hybridization histochemistry, ISHH)，是一种固相分子杂交的方法，它是用标记的DNA或RNA为探针，在原位检测组织或细胞内特定核酸序列的方法。探针的种类按所带标记物可分为同位素
Hybridization－of－High－Density－Arrayed－BAC－Nylon－Filter－Blots
Protocol for hybridization of high density arrayed Bacterial Artificial Chromosome nylon filter blots with 100 PCR isolated Unigene cDNA inserts, pool
多彩色荧光原位杂交技术介绍
多彩色荧光原位杂交（multicolor fluorescence in situ hybridization，mFISH）mFISH是在荧光原位杂交基础上发展起来的新技术，它不仅具有FISH的优点，而且克服了FISH的许多局限，其最大特点是可将多次繁顼的FISH实验和多种不同的基因定位在一次FIS
植物体细胞杂交的相关介绍
植物体细胞杂交（plant somatic hybridization）,又称原生质体融合（Protoplast fusion ）是指将植物不同种、属，甚至科间的原生质体通过人工方法诱导融合，然后进行离体培养，使其再生杂种植株的技术。植物细胞具有细胞壁，未脱壁的两个细胞是很难融合的，植物细胞只有
植物体细胞杂交的概念
植物体细胞杂交（plant somatic hybridization）,又称原生质体融合（Protoplast fusion ）是指将植物不同种、属，甚至科间的原生质体通过人工方法诱导融合，然后进行离体培养，使其再生杂种植株的技术。植物细胞具有细胞壁，未脱壁的两个细胞是很难融合的，植物细胞只有在脱
原生质体融合的基本介绍
植物体细胞杂交（plant somatic hybridization）,又称原生质体融合（Protoplast fusion ）是指将植物不同种、属，甚至科间的原生质体通过人工方法诱导融合，然后进行离体培养，使其再生杂种植株的技术。植物细胞具有细胞壁，未脱壁的两个细胞是很难融合的，植物细胞只有
植物体细胞杂交的技术原理和特点
植物体细胞杂交（plant somatic hybridization）,又称原生质体融合（Protoplast fusion ）是指将植物不同种、属，甚至科间的原生质体通过人工方法诱导融合，然后进行离体培养，使其再生杂种植株的技术。植物细胞具有细胞壁，未脱壁的两个细胞是很难融合的，植物细胞只有在脱
直接原位PCR（In－situ－PCR）操作方法
原位PCR是原位杂交细胞定位和PCR的高灵敏度相结合的技术，使得靶基因检测有了极大的改进。此技术是在细胞（爬片、甩片或涂片）或组织（石蜡、冰冻切片）上直接对靶基因片段进行扩增，通过掺入标记基团直接显色或结合原位杂交进行检测的方法。1．组织切片和细胞样品的制备【试剂与配置】10%福尔马林二甲苯PBS【