SilverAcetateAutometallography(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for light microscopical and ultrastructural autometallographic (AMG) studies. Specific methods were developed to demonstrate endogenous zinc pools in synaptic and secretory vesicles; exogeneous mercury, silver bound as sulfide or selenide crystals, in lysosomes; and tissue-b......阅读全文
Silver－Acetate－Autometallography－(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for l
Silver－Enhancement－...
实验概要The method provides a silver enhancement protocol for immunoassay.主要试剂Prepare the following reagents fresh daily except for the citrate buffer.1.
Silver:－Lysate－for－Western
Grow cellsHarvestSpin downWash with PBSSpin down enough cells to collect a 50-100 µL cell pellet in a FastPrep tubePrepare lysis buffer (beforehand) a
Silver:－Colony－PCR
Zymolyase Solution:Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store aliquots
Silver－Staining－Protocol
1x 40min - overnight 50% MeOH, 12% Acetic Acid1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min
SSR－GEL－and－Silver－Staining－Protocol
I. EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking platformsH
Silver:－TimeLapse－Microscopy
Pad Preparation1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages of a
Protein－Staining－Procedures
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor
SDI检测仪XFsilver
SDI检测仪/污染指数仪/污染指数测定仪 型号：XF-silver 指数（SDI）值，也称之为FI（Fouling Index）值，是水质指标的重要参数之一。它代表了水中颗粒、胶体和其他能阻塞各种水净化设备的物体含量。通过测定SDI值，可以选定相应的水净化技术或设备。 在反渗透水处
SDI检测仪XFsilver
SDI检测仪/污染指数仪/污染指数测定仪 型号：XF-silver 指数（SDI）值，也称之为FI（Fouling Index）值，是水质指标的重要参数之一。它代表了水中颗粒、胶体和其他能阻塞各种水净化设备的物体含量。通过测定SDI值，可以选定相应的水净化技术或设备。 在反渗透水处理
银染(silver－staining)操作规程
实验原理：在碱性条件下，用甲醛将蛋白带上的硝酸银（银离子）还原成金属银，以使银颗粒沉积在蛋白带上。染色的程度与蛋白中的一些特殊的基团有关，不含或者很少含半胱氨酸残基的蛋白质有时候呈负染。银染的详细机制还不是非常清楚。 试剂：乙醇、冰醋酸、乙酸钠、硫代硫酸钠、硝酸银、碳酸钠、甘氨酸或EDTA.Na
10mol/L乙酸胺（ammonium－acetate）配制方法
将77.1g乙酸胺溶解于水中，加水定容至1L后，用0.22um孔径的滤膜过滤除菌。
8mol/L乙酸钾（potassium－acetate）配制方法
8mol/L乙酸钾（potassium acetate）：溶解78.5g乙酸钾于足量的水中，加水定容到100ml。
3mol/L乙酸钠（sodium－acetate）配制方法
溶解40.8g的三水乙酸钠于约90ml水中，用冰乙酸调溶液的pH至5.2，再加水定容到100ml。
醋酸纤维薄膜电泳（cellulose－acetate－membrane－electrophoresis...
实验原理醋酸纤维薄膜电泳是用醋酸纤维薄膜作为支持物的电泳方法。它具有简便、快速、样品用量少，应用范围广，分离清晰，没有吸附现象等优点。目前已广泛用于血清蛋白、脂蛋白、血红蛋白、糖蛋白、多肽、核酸、同工酶及其他生物大分子的分析检测，是医学和临床检验的常规技术。本实验以醋酸纤维素为电泳支持物，分离各种血
低背景的蛋白质银染(silver－staining)方法
我们做蛋白质电泳的人都知道，银染色很灵敏，有很强的说服力，但通常胶背景较深，不易扫描。在这里介绍一下低背景的蛋白质银染方法。Step Reagent Time/minFix 50%EtOH, 12%HAC, 0.1%HCHO, 38%H2O 60Rinse 50% EtOH 5 min, 3 tim
常用试剂配制－4
Phytohemaglutinin (PHA)Available as kidney bean lectin. It is typically used as a stock solution of 10-20 g/ml in balanced salt solution. For tissue c
PCR－clean－up－using－3M－sodium－acetate－and－chilled－absolute－ethanol
For each 20 ul of PCR product, prepare the following mixture in 1.5 ml Tube.2 ul of 3M sodium acetate (NaOAc) pH 4.540 ul chilled absolute ethanol.Tra
醋酸纤维素薄膜(cellulose－acetate－film)分离血清蛋白
原理 采用醋酸纤维素薄膜为支持物的电泳方法，叫做醋酸纤维素薄膜电泳。醋酸纤维素，是纤维素的羟基乙酰化所形成的纤维素醋酸酯。将它溶于有机溶剂（如：丙酮、氯仿、氯乙烯、乙酸乙酯等）后，涂抹成均匀的薄膜则成为醋酸纤维素薄膜。该膜具有均一的泡沫状的结构，有强渗透性，厚度约为120微米。 醋酸纤
Two－Dimensional－Gel－Electrophoretic－Analysis－for－the－Human－Plasma－Proteome
OverviewThis protocol is a detail description of the laboratory procedure in performing 2D gel electrophoresis for illustrating the protein profile of
醋酸氟孕酮（Flurogestone－acetate）ELISA检测试剂盒使用说明
试剂盒只能用于科学研究，不得用于医学诊断醋酸氟孕酮（Flurogestone acetate）ELISA检测试剂盒使用说明书检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被醋酸氟孕酮（Flurogestone acetate）抗体的包被微孔中，依次加入标本、标准品、H
2－Dimensional－Gel－Electrophoretic－Analysis－for－Chicken－Egg
Overview This protocol is a detail description of the procedure in performing 2D gel electrophoresis for illustrating the protein profile of the w
2007年度ScientificComputing杂志“读者选择奖”获奖名单揭晓
“读者选择奖”是由Scientific Computing杂志主办，每年评选一次。读者从软件产品的质量、可靠性、易操作性、技术支持和价格等几方面考虑，从候选名单中选出他们中意的软件。奖项涉及以下几个领域的软件：生物信息学、化学、色谱学、数据采集、数据管理、工程学、制图、成像、数据接口、光谱学、数学、
蛋白质检测
· Protein detection (Aberdeen's Lab)The method used to locate the proteins following 2D-PAGE depends on the nature of the original sample.
加州大学钟声团队：用“一滴血”检测胞外RNA液体活检方法
细胞外RNA（exRNA）作为一种新型液体活检标志物，因其广谱性及多样性近些年引起了科学家的广泛关注。此前研究发现，包括miRNA在内的小exRNA与某些疾病的临床治疗反应相关，但关于其他类型exRNAs的存在情况及其临床相关性研究仍十分有限。此外，血清中的exRNA经过提取步骤后，含量往往非常
MetalEnhanced－Immu...－（一）
实验概要The surface-confined assay format is one of the most convenient detection formats used in many immunoassays. Fluorescence emission from monola
Autoradiography
MaterialsH-Thymidine, specific activity of 2.0curie/millimoleOnion sets, jars and toothpicksAlcohol-acetic acid fixativeMaterials for feulgen reaction
蛋白质电泳
蛋白质电泳（主要内容如下）One-Dimensional SDS-PAGETwo-Demensional SDS-PAGEProtein Electrophoresis in Agarose Gel Gel StainingRecipesOne-Dimensional SDS-PAGE·
组织学——染色
Hematoxylin and Eosin Staining of Tissue for LCM (Arcturus) Immunohistochemical Staining (IHC) (Arcturus)For optimal LCM from IHC samples, it is nec
Detection－of－Glycoproteins－on－Blot
Detection of Glycoproteins on BlotSource: Contributed by Sharad Purohit, Paller's LabReagentsSodium acetate Buffer (200mM, pH 5.5): Prepare a 200