apipelinefortheidentificationofintactNglycopeptides（四）
Interpretation of MS3 data. MS3 data were converted to “.ms3” format by pXtract within pFind Studio (version 2.8)26,27, and then analyzed by pFind 2.8. The protein database was the database of the six standard glycoproteins mixed with 500 protein sequences randomly selected from SwissProt (v12.05, Homo sapiens species) as the background. Concatenated forward-reverse database search was performed to estima......阅读全文
a－pipeline－for－the－identification－of－intact－Nglycopeptides（四）
Interpretation of MS3 data. MS3 data were converted to “.ms3” format by pXtract within pFind Studio (version 2.8)26,27, and then analyzed by pFind
a－pipeline－for－the－identification－of－intact－Nglycopeptides（一）
pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCDand CID-MS/MS and MS3 Wen-Feng Zeng1,2,*, Ming-Qi Liu3,*, Yang Zhang3,
a－pipeline－for－the－identification－of－intact－Nglycopeptides（五）
Alignment between MS/MS and MS3 identifications. Glycans were identified after analyzing the HCD/CID-MS/MS spectrum pairs, but the sequences of pe
a－pipeline－for－the－identification－of－intact－Nglycopeptides（二）
Methods Materials. A uniform mixture of six standard glycoproteins was used as the starting material, including IgG (56834, catalog numeber), IgA
a－pipeline－for－the－identification－of－intact－Nglycopeptides（三）
Figure 1. The overall workflow of pGlyco. First the sample is analyzed by HCD-MS/MS (NCE = 40%).Then the product-dependent CID-MS/MS and data-depend
a－pipeline－for－the－identification－of－intact－Nglycopeptides（六）
Figure 4. Comparison of different decoy strategies. The estimated FDR of each decoy method is compared with the real FDR. “Decoy: + 11” means in
a－pipeline－for－the－identification－of－intact－Nglycopeptides（七）
Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Rece
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites四
Figure 2 Distribution of N-glycopeptides analyzed by different enriched methods and instruments of royal jelly proteins. A is the distribution of N-
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement
IntroductionApoptosis is a normal physiological phenomenon put forward by Kerr [1]. It plays an important role in embryonic development, maintenance o
A－Yeast－Secretion－Trap－Assay－for－Identification－of－Secreted－Proteins－...
Secreted proteins from plants and phytopathogens play important roles in their interactions and contribute to elaborate mechanisms of attack, defe
Identification－and－expansion－of－the－tumorigenic－lung－cancer－stem－cell－...
Identification and expansion of the tumorigenic lung cancer stem cell populationLung cancer contains a rare population of CD133+ cancer stem-like ce
An－Integrative－Procedure－for－Apoptosis－Identification－and－Measurement2
TroubleshootingCritical Steps(1) Don’t trypsinize cells for too long when collecting them.(2) Rotation speed should be no more than 1500 rpm during ce
CarbohydrateSpecific－Adhesion－of－Intact－Cells－to－Resolved－Glycolipids－on－T
Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC PlatesRonald L. Schnaar~Professor, Johns Hopkins University Medical
Interleukin6－Induced－Acute－Phenotypic－Microenvironment－Promote...（三）
Target analysis by Parallel reaction monitoring (PRM)The targeted quantification and verification were carried out in 94 serum samples with 5~6 mic
Identification－of－a－Mutant－Kinase/ATP－Analog－Pair2
Cellular Transfection and Immunoprecipitation Before proceeding with the experiments outlined below, all kinase pocket mutants should be characterize
Identification－of－a－Mutant－Kinase/ATP－Analog－Pair1
Identification of a Mutant Kinase/ATP Analog PairScott T. Eblen, N. Vinay Kumar, and Michael J. WeberDepartment of Microbiology and Cancer Center, Uni
Comparative－assessment－of－glycosylation－of－recombinant－human－...（一）
Comparative assessment of glycosylation of recombinant human FSH and highly purified FSHHong Wang, Xi Chen, Xiaoxi Zhang, Wei Zhang, Yan Li, Hongrui Y
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites一
Comprehensive identification of novel proteins and N-glycosylation sites in royal jellyLan Zhang1,2†, Bin Han1†, Rongli Li1, Xiaoshan Lu1,3, Aiying Ni
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites六
N-glycosylation modification of proteins has reported to improve the health of living organisms through antibacterial activity [68], antioxidant a
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites三
Note: All of the identified proteins are from Apis mellifera. Accession is the unique number given to mark the entry of a protein in the database
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites八
References1. Fujita T, Kozuka-Hata H, Ao-Kondo H, Kunieda T, Oyama M, Kubo T: Proteomic analysis of the royal jelly and characterization of the fu
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites九
41. Schmidt O, Theopold U, Strand M: Innate immunity and its evasion and suppression by hymenopteran endoparasitoids. BioEssays 2001, 23(4):344–35
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites二
ResultsIdentified novel royal jelly proteins To expand the number of known proteins in the RJ proteome, RJ proteins were extracted and digested wit
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites五
RJ provides efficient energetic fuels for the fast development of larvae and the egg-laying queen through the metabolism of sugars, lipids, and pr
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites七
Data analysisTandem mass spectra were retrieved using Xcalibur (version 2.2, Thermo Fisher Scientific) and AnalystTF (version 1.6, AB SCIEX) softw
GenomeWide－Identification－of－Transcription－FactorBinding－Sites－in...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip)
Pipeline血流导向装置治疗颅内大型动脉瘤诊疗分析
随着神经介入技术和器械的发展，中小型颅内动脉瘤的介入治疗效果确切，而大型或巨大型动脉瘤的介入治疗效果不满意，其中重要的原因是动脉瘤复发。近年来，随着载瘤动脉重建概念的提出，一种新型的血流导向装置Pipleline应运而生。有研究表明，血流导向装置主要用于治疗未破裂的宽颈动脉瘤、梭型动脉瘤及大型或巨大
酵母人工染色体
· Easy YAC Preparation Method (Andrew Davies,Shaw lab)· Screening YAC libraries (Donis Keller Lab)This is a method for screening YAC l
Comparative－assessment－of－glycosylation－of－recombinant－human－...（二）
Experimental sectionChemicals and reagentsOne lot of PuregonR-HP of 50 IU/0.5mL and two lots of PuregonR-HP of 100 IU/0.5mL (rhFSH) (Organon, Oss, N
血流导向装置Pipeline治疗以单侧眼睑下垂为首发表现的...
血流导向装置Pipeline治疗以单侧眼睑下垂为首发表现的颈内动脉巨大动脉瘤病例分析Pipeline血流导向装置（Pipeline embolization device，PED）主要用于血管腔内治疗成人颈内动脉岩骨段至垂体上动脉开口处近端的大型或巨大宽颈动脉瘤，对于超适应证范围的，如后循环动脉瘤、