Comparativeassessmentofglycosylationofrecombinanthuman...（一）
Comparative assessment of glycosylation of recombinant human FSH and highly purified FSHHong Wang, Xi Chen, Xiaoxi Zhang, Wei Zhang, Yan Li, Hongrui Yin, Hong Shao, and Gang ChenJ. Proteome Res., Just Accepted Manuscript • DOI: 10.1021/acs.jproteome.5b00921 • Publication Date (Web): 26 Jan 2016Downloaded from http://pubs.acs.org on January 28, 2016 Just Accepted“Just Accepted” manuscripts have been peer-reviewed......阅读全文
Comparative－assessment－of－glycosylation－of－recombinant－human－...（一）
Comparative assessment of glycosylation of recombinant human FSH and highly purified FSHHong Wang, Xi Chen, Xiaoxi Zhang, Wei Zhang, Yan Li, Hongrui Y
Comparative－assessment－of－glycosylation－of－recombinant－human－...（四）
Site-specific characterization of N-glycansFor intact N-glycopeptide analysis, chymotryptic digests of both hFSHs were subjected under UPLC equipped
Comparative－assessment－of－glycosylation－of－recombinant－human－...（二）
Experimental sectionChemicals and reagentsOne lot of PuregonR-HP of 50 IU/0.5mL and two lots of PuregonR-HP of 100 IU/0.5mL (rhFSH) (Organon, Oss, N
Comparative－assessment－of－glycosylation－of－recombinant－human－...（六）
(22) Wu, S. W.; Pu, T. H.; Viner, R.; Khoo, K. H. Novel LC-MS/MS product dependent parallel data acquisition function and data analysis workflow f
Comparative－assessment－of－glycosylation－of－recombinant－human－...（三）
LC-MS data processingN-glycan data were processed using UNIFI 1.7 with Glycobase 3+ (Waters Corporation, Milford, MA) for N-glycan structure. The pe
Comparative－assessment－of－glycosylation－of－recombinant－human－...（五）
Supporting Information(1) RP-UPLC-Q-TOF analysis of hFSHs subunits; (2) molecular weights and possible glycan structures of hFSHs subunits; (3) pr
Comparative－assessment－of－glycosylation－of－recombinant－human－...（七）
Figure 3 MS2 spectra of 2-AB-labeled N-glycan structures. Diagnostic ions are marked with corresponding fragment structures. (a) NeuGc1NeuAc1HexNA
重组人表皮细胞生长因子（recombinant－human－EGF）
生物学功能：人表皮细胞生长因子（hEGF）不仅对上皮细胞而且对许多类型细胞均有促增殖作用，是与细胞的生长及分化有关的重要生长因子。hEGF具有多种生物活性，主要表现在：①在体内刺激皮肤组织、角膜和气管上皮组织的生长繁殖； ②加速角膜、皮肤等表皮创伤的修复； ③抑制胃酸分泌；④促进人皮肤上皮细胞、角膜
Synthesis－of－Recombinant－Human－Cytokine－GMCSF－in－the－－Seeds－of－Transge...
We are interested in studying plant systems as vehicles for the production of recombinant proteins of clinical relevance. There are a number of po
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites一
Comprehensive identification of novel proteins and N-glycosylation sites in royal jellyLan Zhang1,2†, Bin Han1†, Rongli Li1, Xiaoshan Lu1,3, Aiying Ni
流式应用精选——微核检测（体内体外）
注：点击文章名称查看详情。文章名称样本仪器型号备注Assessment of the Genotoxic Potential of Azidothymidine in the Comet,Micronucleus, and Pig-a AssayBone Marrow and Peripheral
高通量测序技术在肠道菌群多样性研究中的应用（三）
一、饮食、能量摄取、肥胖与肠道菌群之间的关系：1. A core gut microbiome in obese and lean twins——2009《Nature》——if 36.102. An obesity-associated gut microbiome with increase
CO2恒温摇床解决人胚肾－293－(HEK293)－细胞结团问题（一）
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白，如 G 蛋白偶联受体 (GPCR) ，是无法在最常见的生物制药生产宿主，如：中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主，然而 HEK293 细胞
In－vitro－Assessment－of－Metabolic－－in－Suspension－Cryopreserved－Hepatocytes
实验概要BackgroundThe pharmaceutical and biotechnology industry’s goal is to discover therapeutic agents that are both safe and effective at treating or
DNA重组技术(Recombinant－DNA)实验原理、用品和步骤（一）
【实验原理】1．DNA重组技术重组DNA(Recombinant DNA)技术是遗传工程的核心技术，也是人类在基因和DNA分子水平进行操作的技术。它包括以下几个步骤： 1）重组DNA分子的构建：即将目的基因（DNA或cDNA片段）与载体DNA重组，应用TA克隆方法,将PCR扩增产物快速克隆至质粒
Glycosylation－of－Antibody－Therapeutics:－Optimisation－for－Purpose
Recombinant antibody therapeutics represent a significant success story in terms of clinical benefit delivered and revenue (profit) generated with
Assessment－of－Magnaporthe－grisea－mating－type－by－spore－PCR
Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In S
欧盟评估一种转基因大豆作为食品和饲料的安全性
2019年11月11日，欧盟食品安全局（EFSA）发布消息，欧盟食品安全局转基因生物小组（GMO Panel）评估了转基因大豆MON 87751×MON 87701×MON 87708×MON 89788用作食品和饲料的安全性。 经过评估，GMO小组得出结论，就对人类和动物健康以及环境的潜在影
Comprehensive－identification－of－novel－proteins－and－Nglycosylation－sites九
41. Schmidt O, Theopold U, Strand M: Innate immunity and its evasion and suppression by hymenopteran endoparasitoids. BioEssays 2001, 23(4):344–35
Production－of－Recombinant－Proteins－in－SuspensionCultured－Plant－Cells
Plants have emerged in the past decade as a suitable alternative to the current production systems for recombinant pharmaceutical proteins and, toda
Preparation－of－human－platelets
Preparation of human platelets 1. Human blood was taken from drug-free volunteers on the day of the experiment using acidic citrate dextrose
Human－Embryonic－Stem－(ES)－Cell－Protocols——Thawing－Human－ES－cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form.Thaw cryovial by gently swirling in waterbath until only a small
Human－Embryonic－Stem－(ES)－Cell－Protocols——Freezing－Human－ES－Cells
Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up).With a 5 ml pipet, gently pipet and scrape colonies f
Steps－in－the－Glycosylation－of－Mammalian－Nlinked－Oligosaccarides
The biosynthesis pathway of N-glycans is a costly system with respect to the number of enzymes that are involved in the synthesis and trimming of N-gl
Purification－of－recombinant－sBRF－M166L
Induction of BRF in bacteria and purification on Ni-NTA agaroseTransform BRF plasmid into strain BL21 DE3 (pLysS) or JM25 (DE3 containing the Arg tRNA
重组质粒(dna－recombinant－plasmid)的连接
质粒具有稳定可靠和操作简便的优点。如果要克隆较小的DNA 片段( ＜10kb) 且结构简单，质粒要比其它任何载体都要好。在质粒载体上进行克隆，从原理上说是很简单的，先用限制性内切酶切割质粒DNA 和目的DNA 片段，然后体外使两者相连接，再用所得到重组质粒转化细菌，即可完成。但在实际工作中，
Characterization－and－Applications－of－PlantDerived－Recombinant－Antibodies
Expression of foreign proteins in plants has become a standard technique in plant molecular biology. Various plant species have been used to produ
Human－Embryonic－Stem－(ES)－Cell－Protocols—Splitting－Human－ES－cells－onMatrige
based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of co
Human－Embryonic－Stem－(ES)－Cell－Protocols——Splitting－Human－ES－cells－on－MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 °C in a water bath.Aspirate media off of cell culture plate.Add the following a
典型CASE分享－蛋白产品常见翻译后修饰（PTM）
翻译后修饰（PTM）是指蛋白质在翻译后发生的化学修饰。抗体在生产、贮存及临床使用过程中，均可能产生各类翻译后修饰变异体。翻译后修饰可能导致抗体所带的电荷乃至结构发生变化，从而影响其与抗原及Fc受体的亲和力，进而影响抗体药物的活性等关键质量属性。因此，对抗体药物的各类翻译后修饰进行表征和工艺控制是有必