AssessmentofMagnaporthegriseamatingtypebysporePCR
Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In Saccharomyces cerevisiae, PCR assay can be performed with yeast colonies (Huxley et al. TIG 1990 6:236). Here we describe a PCR protocol which uses M. grisea conidia directly for PCR analysis without extraction of DNA.The PCR assay is performed with c......阅读全文
Assessment－of－Magnaporthe－grisea－mating－type－by－spore－PCR
Isolation of DNA from filamentous fungi for PCR analysis is usually time consuming and involves use of toxic chemicals such as phenol/chloroform. In S
Transformation－of－Magnaporthe－grisea－to－phosphinothricin－resistance
Three transformation systems have been reported for the rice blast fungus Magnaporthe grisea (Parsons et al. 1987 Proc. Natl. Acad. Sci. USA 84:4161-4
yeast:Assaying－mating
SetupYou have yeast strains that are deficient in mating (eg Ste12 knockouts) and would like to test whether transforming them with a plasmid that con
Streptomyces:Protocols/Spore－Prep
Spore Prep - Inoculating & HarvestingDescription A spore prep is a method of preserving a sporulating strain of Streptomyces. The stock is stored in 2
Performing－a－hunt－by－interaction－mating
AbstractWhen more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt
细菌的芽孢染色(spore－staining)
实验原理细菌的芽胞具有厚而致密的壁，透性低，不易着色，若用一般染色法只能使菌体着色而芽胞不着色（芽孢呈无色透明状）。芽孢染色法就是根据芽孢既难以染色而一旦染上色后又难以脱色这一特点而设计的。所有的芽孢染色法都基于同一个原则：除了用着色力强的染料外，还需要加热，以促进芽孢着色。当染芽孢时，菌体也会着色
Comparative－assessment－of－glycosylation－of－recombinant－human－...（六）
(22) Wu, S. W.; Pu, T. H.; Viner, R.; Khoo, K. H. Novel LC-MS/MS product dependent parallel data acquisition function and data analysis workflow f
Comparative－assessment－of－glycosylation－of－recombinant－human－...（四）
Site-specific characterization of N-glycansFor intact N-glycopeptide analysis, chymotryptic digests of both hFSHs were subjected under UPLC equipped
In－vitro－Assessment－of－Metabolic－－in－Suspension－Cryopreserved－Hepatocytes
实验概要BackgroundThe pharmaceutical and biotechnology industry’s goal is to discover therapeutic agents that are both safe and effective at treating or
Comparative－assessment－of－glycosylation－of－recombinant－human－...（一）
Comparative assessment of glycosylation of recombinant human FSH and highly purified FSHHong Wang, Xi Chen, Xiaoxi Zhang, Wei Zhang, Yan Li, Hongrui Y
Comparative－assessment－of－glycosylation－of－recombinant－human－...（七）
Figure 3 MS2 spectra of 2-AB-labeled N-glycan structures. Diagnostic ions are marked with corresponding fragment structures. (a) NeuGc1NeuAc1HexNA
Comparative－assessment－of－glycosylation－of－recombinant－human－...（三）
LC-MS data processingN-glycan data were processed using UNIFI 1.7 with Glycobase 3+ (Waters Corporation, Milford, MA) for N-glycan structure. The pe
Comparative－assessment－of－glycosylation－of－recombinant－human－...（五）
Supporting Information(1) RP-UPLC-Q-TOF analysis of hFSHs subunits; (2) molecular weights and possible glycan structures of hFSHs subunits; (3) pr
Comparative－assessment－of－glycosylation－of－recombinant－human－...（二）
Experimental sectionChemicals and reagentsOne lot of PuregonR-HP of 50 IU/0.5mL and two lots of PuregonR-HP of 100 IU/0.5mL (rhFSH) (Organon, Oss, N
Primary－type－II－pneumonocyte－Isolation－and－culture
Pulmonary alveolar type II cells carry out highly specialized functions that include the synthesis, secretion, and reutilization of surfactant, a
The－Determination－of－Proteinprotein－Interactions－by－the－Matingbased－...
Dynamic and reversible protein–protein interactions have a pivotal function in all living cells. For instance, protein–protein interactions are in
Hints－and－precautions－for－the－care,－feeding－and－breeding－of－Neurospora2
9. Spontaneous germination. Some genotypes result in spontaneous ascospore germination (for example, the unpigmented ascospores of per-1 Type I). A si
Hints－and－precautions－for－the－care,－feeding－and－breeding－of－Neurospora
Some of these notes describe what may be established routines in older laboratories, but are either unpublished or published in inaccessible places or
Gelelongation－assay－for－type－II－fatty－acid－synthesis
Gel-elongation assay for type II fatty acid synthesisSrinivas KodaliAndrew GalgociSheo Singh Dr.Jun Wang Dr., jun_wang2@merck.com, Merck Research Labo
Twohybrid－analysis－of－genetic－regulatory－networks2
2.2 Interaction mating - large scaleWith a few modifications, the procedure described above can be used to test for interactions between a single prey
Regulation－of－ck1/cdk5－by－type－1－glutamate－receptors
Cdk5 is a cyclin dependent protein kinase involved in dopaminergic signaling in the neostriatal region of the brain. The role of cdk5 in dopamine resp
－Sartorius－Stedim公司推出Type－1的超纯水系统
arium® 611 超纯水(Type 1) 系统 虽然实验室用水有多方面不同的要求，总有一款arium®611可以满足您的需求。 所有arium ® 611机型都有一个共同点：它们卓越的产品性能--专业人士为专业人员所研制。例如，它们的滤芯寿命明显优于竞争对手的产品。不
Twohybrid－analysis－of－genetic－regulatory－networks
1. Introduction and BackgroundThere is a great need for general methods to characterize the proteins that contemporary biology makes available. The li
A－practical－method－for－the－preparation－of－total－DNA－from－filamentous－fungi
Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chlori
日本新东HEIDON－Type18/18L连续加载式抗刮
日本新东HEIDON Type18/18L连续加载式抗刮强度试验机● 用连续加载法将抗刮强度数值化 划针种类使用在尖部做了圆弧加工（0.005～1.0mm各种型号）的圆锥形划针。在机器臂上滚动的砝码与装载试件的移动台一起运动，与此同时对划针给予连续加载。 移动距离为100mm，通过调换
PNAS：细菌抵御病毒侵染Type－I－RM系统的新发现
中科院生物物理研究所梁栋材院士课题组的研究论文 “Structural basis underlying complex assembly and conformational transition of the Type I R-M system”。该项工作报道了细菌TypeI系统中核心酶MT
Streptomyces:Protocols/Conjugation
Intergeneric Conjugation and OverlayDescription Transfer of plasmid/cosmid DNA from a host strain, e.g. E.coli ET12567 [pUZ8002], to the recipient str
定量RTPCR－（Quantitative－RTPCR）
Application: Quantitative RT-PCR is used to quantify mRNA in both relative and absolute terms. It can be applied for the quantification of mRNA expres
Competitive－RTPCR－Strategy－for－Quantitative－Evaluation－1
Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type IQuantizatio
酵母双杂交系统
· Yeast Two-Hybrid System (Finley Lab)This is one of the most comprehensive and detailed guide to yeast two-hybrid system technique with intro