3HThymidineUptakebyCulturedCells
Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)Subbed slides (coated with chrom alum gelatin) and PermountNuclear Track Emulsion (Kodak or Ilford)Darkroom and chemicals for photographic processingDektol developerKodak FixerGiemsa stain, graded series of alcohols, xylol Procedure Grow either L cells (Mouse fibro......阅读全文
3HThymidine－Uptake－by－Cultured－Cells
3H-Thymidine Uptake by Cultured Cells OverviewA radioactive method for measuring cultured cell growth with 3H-thymidine is described here. MaterialFi
3HThymidine－Uptake－by－Cultured－Cells
Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)
HThymidine－Uptake－by－Cultured－Cells
H-Thymidine Uptake by Cultured CellsLEVEL IIMaterialsFibroblast cells in log phase growthCa, Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaral
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Articles posted in the Method Froum Cell Viability AssayDye exclusion method Viable Cell Counts Using Trypan Blue (Gibco) Soft Agar Assay For Colo
Fixation－of－Cells－Cultured－in－Transwell－Dishes
Fixation of Cells Cultured in Transwell DishesTranswell culture dishes are commonly used to culture cells so that the top and bottom of the cells can
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IntroductionFluorescence in situ hybridization (FISH) has become a widely used method in genome and molecular genetic studies. The technique is highly
RNA－FISH－on－cultured－cells－in－interphase2
Post-labeling DNA processing and purificationQiagen PCR clean up to get rid of unused oligonucleotides;Add 20µl cot1DNA, 10µl ssDNA to compete for rep
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45Ca2+ uptake study in PC12 cells 1. Plate the cells on to the poly-D-Lysine-coated six well plates 2 days prior to study. 2. Rinse the cells with 1 m
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CMVE were isolated from Wistar rat hearts by digestion of primary cell isolation kit.1. Hearts mounted on a Langendorff apparatus were perfused at 3
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ADSC isolation, culture, and cloningRat amnion membrane is mechanically separated from the chorion of embryonic day 18.5 Sprague–Dawley rat embryosThe
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Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.___________________Day 1: Trypsiniz
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Culture of BEND Cells (Bovine Endometrial Cells)Charles E. Krininger, III and Peter J. Hansen Dept. of Animal Sciences, University of FloridaThis prot
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1. A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2. The patients were proven to be no H.
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There are many procedures with which to trypsinize cells. All include washing the cell monolayer with TD, or in rare cases, with VE. This removes seru
Lyophilizing－Cells
Inoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized.Incubate the culture at 37°C with vigorous shak
Lyophilizing－Cells
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Freezing－Cells
1) Keep prepared solutions on ice.2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 )3) Determine number of vials to be frozen. (e.g.
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No two cell lines behave exactly the same, so you must learn the peculiarities, or personality, of each of the cell lines with which you work. Irrespe
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Mitochondrion-Selective Rhodamines and RosaminesRhodamine 123Rhodamine 123 (R302; FluoroPure Grade, R22420; Figure 12.22) is a cell-permeant, cationic
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Plants have emerged in the past decade as a suitable alternative to the current production systems for recombinant pharmaceutical proteins and, toda
体外荧光法检测核内体早期动力学
A fluorescence-based in vitro assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1 & Silvio O Rizzoli2ABSTRACTEarly endoso
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体外荧光法检测核内体早期动力学5
Leave tubes on ice and repeat Steps 13–15 for the next 6–12 plates of cells.When all cells are collected, centrifuge all tubes at 250g for 5 min at 4
Preparing－cells－and...
实验概要The method provides a protocol and tips for BrdU staining in tissue sections.Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic
Subculturing－Adherent－Cells
实验概要The following protocol describes a general procedure for subculturing adherent mammalian cells in culture.主要试剂1. Complete growth medium, pre-warme