45Ca2+uptakestudyinPC12cells
45Ca2+ uptake study in PC12 cells 1. Plate the cells on to the poly-D-Lysine-coated six well plates 2 days prior to study. 2. Rinse the cells with 1 ml secretion buffer (150 mM NaCl, 5mM KCl, 2mM CaCl2, 10 mM Hepes, pH 7.4) every 15 minutes for 1 hour at 37oC. 3. Add 45Ca2+ (2 µCi/ml) to Ca2+ -free buffer (secretion buffer without 2 mM CaCl2), and drugs to the labelled buffer at 37oC. 4. Incubate ......阅读全文
45Ca2+－uptake－study－in－PC12－cells
45Ca2+ uptake study in PC12 cells 1. Plate the cells on to the poly-D-Lysine-coated six well plates 2 days prior to study. 2. Rinse the cells with 1 m
HThymidine－Uptake－by－Cultured－Cells
H-Thymidine Uptake by Cultured CellsLEVEL IIMaterialsFibroblast cells in log phase growthCa, Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaral
3HThymidine－Uptake－by－Cultured－Cells
Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)
3HThymidine－Uptake－by－Cultured－Cells
3H-Thymidine Uptake by Cultured Cells OverviewA radioactive method for measuring cultured cell growth with 3H-thymidine is described here. MaterialFi
mpulsive－Pressurization－of－Neuronal－Cells－for－Traumatic－Brain－Injury－Study
实验概要A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic brain injur
体外荧光法检测核内体早期动力学4
Top of pageProcedureOverviewSteps 1 - 8 Preparation of rat brain cytosolSteps 9 - 10 Culture of PC12 cellsSteps 11 - 30 Preparation of postnuclear sup
体外荧光法检测核内体早期动力学
A fluorescence-based in vitro assay for investigating early endosome dynamicsSina V Barysch1,2, Reinhard Jahn1 & Silvio O Rizzoli2ABSTRACTEarly endoso
New－Case－Study－Released
The Dutch grocery retailer Spar decided to manage all its retail weighing scales centrally from the company’s headquarters using the METTLER TOLED
A－study－of－Fetal－Alcohol－Syndrome
IntroductionFetal Alcohol Syndrome (FAS) is caused by exposure of the developing embryo to alcohol, one of several teratogenic agents which adversely
PC12细胞培养
PC12细胞是大鼠肾上腺髓质嗜铬瘤分化细胞株，具神经内分泌细胞的一般特征，因其具可传代特点，广泛应用于神经生理和神经药理学研究。1. PC12细胞有两种：未分化型和分化型。其中未分化型的PC12细胞贴壁能力强，传代时需要胰酶消化，形状不规则。分化型PC12细胞传代时不需要胰酶，直接可以吹下来，形状规
PC12－Cell－Culture－and－Fusion
Cell CultureMaterials1. Falcon Primaria culture dishes.2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse se
Green－Fluorescent－Protein－as－an－Indicator－ofTransfection－in－Chicken－Embryos
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kD
PC12细胞培养方法步骤
PC12细胞是大鼠肾上腺髓质嗜铬瘤分化细胞株，具神经内分泌细胞的一般特征，因其具可传代特点，广泛应用于神经生理和神经药理学研究。1. PC12细胞有两种：未分化型和分化型。其中未分化型的PC12细胞贴壁能力强，传代时需要胰酶消化，形状不规则。分化型PC12细胞传代时不需要胰酶，直接可以吹下来，形
线粒体肿胀检测方法
Estimating Mitochondrial Mass，可以用于线粒体肿胀的检测Accurate measurements of mitochondrial mass require a probe that will accumulate in mitochondria regardless
Culture－of－BEND－Cells－(Bovine－Endometrial－Cells)
Culture of BEND Cells (Bovine Endometrial Cells)Charles E. Krininger, III and Peter J. Hansen Dept. of Animal Sciences, University of FloridaThis prot
Differentiate－ES－cells－into－glial－cells－and－neurons
Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.___________________Day 1: Trypsiniz
软件：Genomewide－Association－Study－Software
The software included are listed bellow IMPUTEa program for genotype imputation in genome-wide association studies and fine-mapping studies based on a
Optimized－Fibrin－Gel－Bead－Assay－for－the－Study－of－Angiogenesis
PREPARING CELLSBring up HUVEC and fibroblasts in M199/10% FBS/Pen-Strep (1:100) 1-2 days before beading.Switch medium to EGM-2 (Clonetics) the day bef
线粒体荧光探针大全：TMRM，Mitotracker，JC1（3）
Mitochondrion-Selective Rhodamines and RosaminesRhodamine 123Rhodamine 123 (R302; FluoroPure Grade, R22420; Figure 12.22) is a cell-permeant, cationic
Differentiating－Neural－Stem－Cells－into－Neurons－and－Glial－Cells
实验概要The protocols in this section describe the steps involved in differentiating neural stem cells (NSC) to neurons, astrocytes, and oligodendrocyte
Use－of－Nonaqueous－Fractionation－and－Metabolomics－to－Study－Chloroplast－...
Use of Non-aqueous Fractionation and Metabolomics to Study Chloroplast Function in ArabidopsisChloroplasts are the chemical factories of plant cells b
Growing－cells
No two cell lines behave exactly the same, so you must learn the peculiarities, or personality, of each of the cell lines with which you work. Irrespe
Trypsinizing－cells
There are many procedures with which to trypsinize cells. All include washing the cell monolayer with TD, or in rare cases, with VE. This removes seru
Lyophilizing－Cells
Lyophilizing CellsInoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized.Incubate the culture at 37°C
Lyophilizing－Cells
Inoculate 200 ml L-broth supplemented with appropriate antibiotics with the bacteria to be lyophilized.Incubate the culture at 37°C with vigorous shak
Freezing－Cells
1) Keep prepared solutions on ice.2) Determine total cell count of cells to be frozen. (e.g. 1 X 108 )3) Determine number of vials to be frozen. (e.g.
BTG－family－proteins－and－cell－cycle－regulation
BTG2 is found to be one of the immediate early genes up-regulated by neural growth factor (NGF) and epidermal growth factor (EGF). Its transcriptional
柴之芳：Hybrid－MS－in－metallomics－and－metalloproteomics：A－case－study
中国科学院高能物理研究所 柴之芳院士 2014年4月26日，首届全国质谱分析学术研讨会在北京西郊宾馆盛大开幕。来自中国科学院高能物理研究所的柴之芳院士带来了题为《Hybrid MS in metallomics and metallo
体外荧光法检测核内体早期动力学5
Leave tubes on ice and repeat Steps 13–15 for the next 6–12 plates of cells.When all cells are collected, centrifuge all tubes at 250g for 5 min at 4
PC12细胞培养所用血清问题
问：我正准备购买上海细胞所的未分化的PC12细胞，需要灭活的马血清，可现在买血清很困难，好像是海关有封锁，代理商这么说的，我原定购买的Gibco的horse surum，现在无法买到了，好容易找到一个目前可以进血清的公司Hyclone，有一种Donor Equine serum是马血清吗？答：H