Isolationandgrowthofmouseprimarymyoblasts
Isolation of limb muscle from neonatal mice1. Neonatal mice by decapitation or CO2 inhalation.2. Rinse the limbs with 70% ethanol and remove them with sterile scissors. Dissect the muscle away from the skin and bone with sterile forceps.Dissection is easier if done under a stereo dissecting microscope. Store the muscle tissue in a culture dish on ice in a drop of sterile PBS as successive limbs ar......阅读全文
Isolation－and－growth－of－mouse－primary－myoblasts
Isolation of limb muscle from neonatal mice1. Neonatal mice by decapitation or CO2 inhalation.2. Rinse the limbs with 70% ethanol and remove them wi
ISOLATION－OF－PRIMARY－MOUSE－EMBRYO－FIBROBLASTS
You will need:13.5 day pregnant mouse (we use MTK NEO inbred white mice)2 sets sterile instrumentsone containing a pair of curved forceps and a pair o
Isolation－of－mouse－embryos
1. Sacrifice impregnated mouse.2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forceps,use another to tear the mesome
RAT/MOUSE－GROWTH－HO...
实验概要This Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive quantification of Growth Hormone in rat or mouse serum, plasma, tissue
Primary－brain－cell－isolation－and－culture
1. Cerebella were removed from 7-day-old mice and passed through Nitex nylon netting (80 μm pore size) into primary cell system containing 20% (v/
Primary－cardiac－fibroblast－and－cardiomyocyte－isolation
1. Ventricles were removed under sterile conditions. 2. Ventricles were placed in cold sterile primary cell culture medium, minced into approximat
Isolation－of－human－primary－airway－smooth－muscle
1. Primary cultures of human primary airway smooth muscle (ASM) cells were prepared from explants of airway smooth muscle. 2. The human trachea tiss
Primary－type－II－pneumonocyte－Isolation－and－culture
Pulmonary alveolar type II cells carry out highly specialized functions that include the synthesis, secretion, and reutilization of surfactant, a
Isolation－and－growth－of－pulmonary－artery－adventitial－fibroblasts
1. Adventitia from the main pulmonary artery was harvested neonatal calves. 2. Tissue was collected, carefully dissected free of blood vessels and f
Isolation－of－human－primary－ovarian－surface－epithelial－cells
1. Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (References)
Isolation－of－human－primary－gastric－mucosa－epithelial－cells
1. A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2. The patients were proven to be no H.
Mouse－B－cell－isolation－with－magnetic－beads
实验概要Isolate B cells from total mouse cells实验步骤 Praparation:1. Enough MACS; put RPMI into water bath.2. Put some MACS in 15 ml tube or 1.5
Isolation－of－lymphatic－endothelial－cells
实验概要This protocols provides a general protocol for isolation of lymphatic endothelial cells.实验步骤Dermal Cell Suspensions1. Dermatomed 0.8-mm split-thic
Isolation－of－lymphatic－endothelial－cells
Dermal Cell Suspensions 1. Dermatomed 0.8-mm split-thickness skin was obtained from adult healthy individuals undergoing elective surgery. 2
Isolation－of－human－corneal－endothelial－cells－(HCECs)
Isolation of HCECs1. The corneoscleral tissues were rinsed three times with primary cell culture system containing 50 mg/mL gentamicin and 1.25 mg
Freezing－and－Thawing－of－MEFs
Author: Shalini Jain and Hariom YadavAffiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, IndiaDate A
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs)1. The human pulmonary arteries were opened to expose the endothelial su
胚胎干细胞培养
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routine Splitting and freezing of cells (
Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture
Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w
细胞组织消化常用的几种酶的选择
直接从生物体获取的组织，一般需要将其消化成单个细胞才能进行体外培养。这种直接从离体组织获得的细胞，更接近于生物体内的生活状态，且生物性状尚未发生很大改变，因此在药物筛选、细胞移植、类器官培养、肿瘤研究等众多领域备受欢迎。但组织消化过程中常遇到多种问题，例如消化不完全、细胞死亡率高等。如何克服这些问题
Isolation－and－longterm－cultivation－of－Human－Corneal－Endothelial－Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
Culture－of－retinal－endothelial－cells－and－pericytes
Isolation of retinal microvessels1. Eyes were obtained and transported on ice.2. Eyes were cut circumferentially 3 mm posterior to the limbus, the v
Culturing－Mouse－Embryonic－Fibroblasts
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium + 20%
胚胎干细胞培养技术大全
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURERoutine Culturing of ES CellsISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTSMITOMYCIN C TREATMEN
Trabecular－cell－monolayer－culture
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1. Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2. All women reported
Primary－cultures－of－intrahepatic－bile－duct－epithelial－cells－isolated－and...
Primary cultures of intrahepatic bile duct epithelial cells isolated and cultured1. Liver was surgically removed and perfused via the hepatic vein.2.
GentleMACS/单细胞悬液制备文献集锦
种属 处理组织 文章名称 human
In－vitro－growth－of－seedlings
sterilisation of seeds: rinse with 70% EtOH for 30 sec put in 1% bleach (sodium hypochlorite, supplemented by a few drops of Tween-20) for 5 mi
Agrobacterium－growth－and－transformation
Growth and storage of Agrobacterium tumefaciensStrain GV3101: resistant to gentamycin and rifampicin so add 25-50 ug/ml Gentamycin, 10 ug/ ml rifampic