Isolationandgrowthofpulmonaryarteryadventitialfibroblasts
1. Adventitia from the main pulmonary artery was harvested neonatal calves. 2. Tissue was collected, carefully dissected free of blood vessels and fat under a dissecting microscope, and then cut into small pieces. 3. Adventitial tissue was isolated, carefully dissected free of blood vessels and fat under a dissecting microscope, and cut into small pieces. 4. The tissue pieces were incubate......阅读全文
Isolation－and－growth－of－pulmonary－artery－adventitial－fibroblasts
1. Adventitia from the main pulmonary artery was harvested neonatal calves. 2. Tissue was collected, carefully dissected free of blood vessels and f
Isolation－and－culture－of－human－pulmonary－artery－smooth－muscle－cells－...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs)1. The human pulmonary arteries were opened to expose the endothelial su
Keratocyte－isolation－(corneal－fibroblasts)
Corneal keratocytes (corneal fibroblasts) that this corneal layer is specialized fibroblasts residing in the stroma, representing about 85-90% of
Isolation－of－Human－Pulmonary－Epithelial－Cells
Isolation of Human Pulmonary Epithelial Cells 1. Pulmonary epithelial cells from human lung tissue were isolated.2. Lungs were perfused with 10
ISOLATION－OF－PRIMARY－MOUSE－EMBRYO－FIBROBLASTS
You will need:13.5 day pregnant mouse (we use MTK NEO inbred white mice)2 sets sterile instrumentsone containing a pair of curved forceps and a pair o
Isolation－of－rat－cardiac－fibroblasts－and－cardiomyocytes
1. Hearts were removed from newborn rats (day 0), put into calcium- and bicarbonate-free HEPES-buffered Hanks’ medium, cut into pieces and digeste
Isolation－and－growth－of－mouse－primary－myoblasts
Isolation of limb muscle from neonatal mice1. Neonatal mice by decapitation or CO2 inhalation.2. Rinse the limbs with 70% ethanol and remove them wi
Primary－Culture－of－Pulmonary－Arterial－Smooth－Muscle－Cells
Primary Culture of Pulmonary Arterial Smooth Muscle Cells 1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from hum
Isolation－and－cultivation－of－endothelial－progenitor－cells－(EPCs)
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
Bacteria－Growth－and－Culture－Bacteria－Growth－and－Culture
Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w
Virus－Infection－of－Fibroblasts
Procedure: Day One 1. Plate cells in 60 mm dishes using 5 ml DMEM containing 10% Calf Serum. The protocol has worked well for NIH3T3 with 1 x 105 cell
Virus－Infection－of－Fibroblasts
Procedure: Day One 1. Plate cells in 60 mm dishes using 5 ml DMEM containing 10% Calf Serum. The protocol has worked well for NIH3T3 with 1 x 105 cell
PriCells:－Isolation－of－endothelial－progenitor－cells－(EPCs)
PriCells: Isolation of endothelial progenitor cells (EPCs) 1. Twenty-four mL venous blood was collected at each time point into Vacutainer CPT Mono
Culturing－Mouse－Embryonic－Fibroblasts
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium + 20%
Rat－Lung－Perfusion
实验概要The procedure presented below describes a method for perfusing rat lung.主要试剂Phosphate Buffered Saline主要设备1.Dissecting Microscope or Eye Magnifier2
In－vitro－growth－of－seedlings
sterilisation of seeds: rinse with 70% EtOH for 30 sec put in 1% bleach (sodium hypochlorite, supplemented by a few drops of Tween-20) for 5 mi
Agrobacterium－growth－and－transformation
Growth and storage of Agrobacterium tumefaciensStrain GV3101: resistant to gentamycin and rifampicin so add 25-50 ug/ml Gentamycin, 10 ug/ ml rifampic
Primary－cardiac－fibroblast－and－cardiomyocyte－isolation
1. Ventricles were removed under sterile conditions. 2. Ventricles were placed in cold sterile primary cell culture medium, minced into approximat
Freezing－and－Thawing－of－MEFs
Author: Shalini Jain and Hariom YadavAffiliation: Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, IndiaDate A
Isolation－and－longterm－cultivation－of－Human－Corneal－Endothelial－Cells
Coating of the Culture Dish SurfacesCulture dishes were incubated with a film of a 1:1 mixture of laminin (10 μg/ml and chondroitin sulfate (10 μg
Growth－Hormone－Signaling－Pathway
Growth hormone plays a major role in regulating growth during childhood and adolescence and also regulates metabolism. Defects in growth hormone signa
胚胎干细胞培养
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routine Splitting and freezing of cells (
Primary－type－II－pneumonocyte－Isolation－and－culture
Pulmonary alveolar type II cells carry out highly specialized functions that include the synthesis, secretion, and reutilization of surfactant, a
应用超声心动图诊断肺动脉吊带病例分析
病例 男，20月，以“发现心脏杂音9月”为主诉入院。查体：胸骨左缘2-3肋间可闻及Ⅲ级收缩期杂音。超声心动图：主肺动脉（Main pulmonary artery，MPA）直接延续为右肺动脉（Right pulmonary artery，RPA），肺动脉分叉未显示，RPA发出约1 cm处可见
GentleMACS/单细胞悬液制备文献集锦
种属 处理组织 文章名称 human
RAT/MOUSE－GROWTH－HO...
实验概要This Rat/Mouse Growth Hormone ELISA kit is used for the non-radioactive quantification of Growth Hormone in rat or mouse serum, plasma, tissue
Exercise－12.9－－Eukaryote－Growth－Dynamics
Exercise 12.9 - Eukaryote Growth DynamicsLEVEL IIMaterialsSuspension cultures set up from Exercise 12.8Sterile transfer pipettesMaterials for viabilit
Bacteria,－cont.－Growth－and－Reproduction
Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w
Nerve－growth－factor－pathway－(NGF)
Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture NGF induces the f
Signaling－of－Hepatocyte－Growth－Factor－Receptor
The hepatocyte growth factor receptor, also called c-Met, is activated by HGF and stimulates proliferation of hepatocytes and other cell types. Mutate