Barrettsesophagealepithelialandfibroblastprimarycultures
1. Biopsy specimens for tissue culture were immediately placed on ice in primary cell culture system. 2. Within 4 hours from the time of the biopsy, the specimens were minced into approximately 2-mm3 fragments. 3. Primary cell cultures were initiated by maintaining the cells in primary epithelial cell system primary epithelial medium 10% fetal bovine serum (FBS) &nbs......阅读全文
Barretts－esophageal－epithelial－and－fibroblast－primary－cultures
1. Biopsy specimens for tissue culture were immediately placed on ice in primary cell culture system. 2. Within 4 hours from the time of the biopsy, t
Primary－cultures－of－intrahepatic－bile－duct－epithelial－cells－isolated－and...
Primary cultures of intrahepatic bile duct epithelial cells isolated and cultured1. Liver was surgically removed and perfused via the hepatic vein.2.
Primary－Cultures－fo...
实验概要The following protocol provides a method of primary cultures for IHC – viability assays.实验步骤1. Preparation of primary mesencephalic cultures 1)
Primary－cardiac－fibroblast－and－cardiomyocyte－isolation
1. Ventricles were removed under sterile conditions. 2. Ventricles were placed in cold sterile primary cell culture medium, minced into approximat
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose:A: Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspected
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
实验概要 Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspect
Isolation－of－human－primary－ovarian－surface－epithelial－cells
1. Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institutional Review (References)
Isolation－of－human－primary－gastric－mucosa－epithelial－cells
1. A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2. The patients were proven to be no H.
Isolation－of－normal－mammary－epithelial－cells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagen
Isolation－of－human－prostatic－epithelial－cells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagen
Rat－urinary－bladder－urothelial－cells
1. Bladders were excised from deeply anesthetized (urethane, 1.2 gm • kg−1, i.p.) Sprague Dawley rats (of either sex), cut open, and gently stretc
Isolation－of－human－endometrial－epithelial－cells
Tissue collection1. Endometrial biopsies were collected from women undergoing gynaecological procedures for benign conditions. 2. All women reported
Isolation－of－rat－cardiac－fibroblasts－and－cardiomyocytes
1. Hearts were removed from newborn rats (day 0), put into calcium- and bicarbonate-free HEPES-buffered Hanks’ medium, cut into pieces and digeste
Different－types－of－human－cells
Human primary cells are cells taken from living human beings and cultured. These cells retain the differentiation of the original cells taken in
Primary－type－II－pneumonocyte－Isolation－and－culture
Pulmonary alveolar type II cells carry out highly specialized functions that include the synthesis, secretion, and reutilization of surfactant, a
Culture－of－rheumatoid－arthritis－synoviocytes－and－collection－of－synovial－...
Culture of rheumatoid arthritis synoviocytes and collection of synovial fluidsCulture of rheumatoid arthritis synoviocytes and collection of synovial
Barrett食管预防
Barrett食管改变生活方式是预防泛酸烧心的最好办法，尽量少吃高脂肪餐、巧克力、咖啡、糖果、红薯、土豆、芋头；严格戒烟和停止饮酒；少吃多餐，餐后不宜马上躺下，睡 前2～3小时最好不要进食；如果晚上容易反酸，最好在睡眠时把床头抬高10～20厘米；另外，心理因素也十分重要，像焦虑、抑郁都会让消
Barrett食管治疗
1.药物治疗 （1）质子泵抑制剂（PPIs） 为内科治疗首选药物，剂量宜较大，如奥美拉唑、泮妥拉唑、雷贝拉唑、埃索美拉唑等。症状控制后以小剂量维持治疗。有证据表明，PPIs长期治疗后可缩短Barrett黏膜长度，部分病例BE黏膜上有鳞状上皮覆盖，提示PPIs能使BE部分逆转，但很难
Barrett食管检查
1.X 线检查 较难发现Barrett食管，有食管裂孔疝及反流性食管炎的表现，不是此症的特异性。发现食管有消化性狭窄或体部有溃疡者应疑有Barrett食管。 2.食管镜检查 典型的Barrett食管是在胃食管交界上方出现红色的柱状上皮区，部分患者可见反流性食管损伤的征象。活
Primary－Culture－of－Pulmonary－Arterial－Smooth－Muscle－Cells
Primary Culture of Pulmonary Arterial Smooth Muscle Cells 1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from hum
Isolation－of－human－uterine－smooth－muscle－cells
1. Approval to utilize human tissue was given by Hospital's Committee on Research Involving Human Subjects. 2. Human myometrial and leiomyoma tiss
Microaspiration－of－Esophageal－Gland－Cells－and－cDNA－Library－Construction－for
Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic NematodesIdentifying p
ORGANOTYPIC－KIDNEY－CULTURES
-embryos are dissected from timed-pregnant mice from 11.5 d.p.c. to 13.5 d.p.c.-metanephroi and associated ureteric buds are microdissected and placed
Hippocampal－Neuron－Cultures
实验概要The protocol provides a method of hippocampal neuron cultures.主要试剂Begin by timing the pregnant mouse at E17-E19 days of gestation. Have ready the
Mouse－keratinocyte－cultures
PRIMARY MOUSE KERATINOCYTE CULTURESIsolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods Enzymol
ORGANOTYPIC－LIMB－CULTURES
-embryos are dissected from timed-pregnant mice from 10.5 - 11.5 d.p.c.-limb buds are microdissected and placed in holding medium (L15 medium suppleme
Preserving－yeast－cultures
Short term storageYeast cultures are stable for 1-2 weeks when refrigerated. Petri dishes should be sealed or in plastic bags.Medium term storageYeast
Growing－Overnight－Cultures
1. Place 2 mL of the appropriate sterile medium in a 13 mm yellow-capped culture tube. If more culture is needed, place up to 5 mL in a 16 mm green-ca
Cryopreservation－of－cell－cultures
1. Examine all flasks by inverted-phasemicroscopy. Cultures used for preservation should be grown free of antibiotics, show no signs of microbial cont
Growing－Cells－in－Geltrex™－Reduced－Growth－Factor－Basement－Membrane－Matrix
实验概要Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or