I. Embryo Preparation1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo by carefully grabbing and pulling with fine forceps. Keep embryos in HBStcontaining antibiotics until use.2) Prepare 1 % agarose-coated operating dish, rinse with HBSt and antibiotics.3)Transfer donor and recipient embryos into operating dish containing 1......阅读全文
I. Embryo Preparation 1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embr
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fi
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mo
Objective This experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogene
Background Since different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
许多蝾螈可以很容易地再生失去的肢体,但成年哺乳动物,包括人类,并不能。为什么会出现这种情况是一个科学谜团,数千年来一直吸引着自然界的观察者。 现在,缅因州巴尔港MDI生物实验室的James Godwin博士领导的一个科学家团队,通过发现促进高度再生蝾螈axolotl再生的分子信号差异,同时阻断
Objective In this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate
ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of ax
EMBRYO CELL AND TISSUE CULTURE TECHNIQUES Rearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solutio