EmbryoDissectionandMicromanipulationTools
Embryo Dissection and Micromanipulation ToolsHazel L. Sive, Robert M. Grainger, and Richard M. HarlandAdapted from "Equipment for Embryo Experiments," Chapter 4, in Early Development of Xenopus laevis by Hazel L. Sive, Robert M. Grainger, and Richard M. Harland. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000.INTRODUCTIONThis article describes the creation and......阅读全文
Embryo－Dissection－and－Micromanipulation－Tools
Embryo Dissection and Micromanipulation ToolsHazel L. Sive, Robert M. Grainger, and Richard M. HarlandAdapted from "Equipment for Embryo Experiments,"
Embryo－Lysates－－Immunoprecipitation
Embryo lysatesTake 25 embryos and place into 1.7ml centrifuge tube.Rinse once in lysis buffer (add ~ 1ml) and remove by aspirationAdd 500 µL lysis buf
Chick－Embryo－staging
In order to repeat a published experiment, or have someone else repeat yours, it is important to use the same materials. For developmental studies, th
MEDIA－FOR－EMBRYO－CULTURE－AND－MANIPULATION
M16 Medium (Protocol obtained from Karen Austen-Reed from SS Tan Laboratory, Anatomy Department)For oocyte maturation and routine culture of mouse emb
Immature－Embryo－Rescue－and－Culture
Embryo culture techniques have many significant applications in plant breeding, as well as basic studies in physiology and biochemistry. Immature
MEDIUM－FOR－EMBRYO－RESCUE－(FOR－1－L)
Put 500 mL of distilled water in a 1-L beaker.Add the following:ucrose (6 % final concentration) – 30 gi-inositol (100 mg/L stock is 0.5 g/50 mL) – 10
ISOLATION－OF－PRIMARY－MOUSE－EMBRYO－FIBROBLASTS
You will need:13.5 day pregnant mouse (we use MTK NEO inbred white mice)2 sets sterile instrumentsone containing a pair of curved forceps and a pair o
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d
Slice－and－Explant－Culture－Protocols－–－Hevner－lab－2002
for axon tracing & cell culture studies in vitro using embyros age E11.5 – E16.5modified from Rubenstein lab and Price lab protocols1. Setupa. 2 hr be
Human－Alkaline－Phosphatase－staining－of－transgenic－embryo/tissue
AP Buffer:100 mM Tris-HCl, pH 9.5100 mM NaCl10 mM MgCl2PTM:PBS0.1% Tween-202 mM MgCl2Since this is generally done in conjunction with lacZ staining, e
Chorioallantoic－membrane－grafting－with－chick－embryo－limb－buds
ObjectiveThis experiment explores the ability of the chick chorioallantoic membrane (CAM) to support an excised limb bud from a donor embryo. The chic
Transplantation－of－theeyeforming－region－of－Amphibian－neurula－into－theflank
EMBRYO CELL AND TISSUE CULTURE TECHNIQUESRearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solution.Micro
Developmental－Effects－of－Transplantation－of－Cell
From the various microsurgical procedures of Spemann and others, several developmental principles have emerged about amphibian embryos. One of the mos
Derivation－and－Culture－of－Dopaminergic－Neurons－(from－Midbrains－of－Rodents)
实验概要Dopaminergic (DA) neurons are located in the ventral midbrain (VM). The ability to isolate precursor cells and neurons from the VM provides a po
Isolation,－Culture,－and－Differentiation－of－Progenitor－Cells
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous SystemScott R. Hutton and Larysa H. Pevny1UNC Neuroscience Center
In－VitroCulture－of－Chicken－Heart
The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in g
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
Axolotl－Protocol:－Transplantation－of－the－Gill－Organ－Field
Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ fie
Axolotl－Microsurgery
I. Embryo Preparation1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo
The－effects－ofdifferent－concentrations－oflithium－chloride－on－thedevelopment
AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos results in
Regulative－Development－in－Axolotl－Embryos;－Splitting－the－Heart－Field
ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically, we will split the morphogenetic
多功能胚胎激光破膜仪（二）
A new approach to cyropreservation of large equine embryos by vitrification after blastocoel micromanipulation.J. Scherzer, R. A. Fayrer-Hosken, L. Ra
Culturing－Mouse－Embryonic－Fibroblasts
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium + 20%
Observation－of－living－and－plasticembedded－chick－embryos
The development of chick embryos has been studied since Aristotle. It is one of the most intensely studied organisms. One reason for this is that ther
The－Effects－of－Ethanol－on－Zebrafish－Development
Introduction:The early stages of zebrafish, Danio rerio development are characterized by meroblastic dicoidal cleavage. Initially cell division only o
Effect－of－ethanol－on－development－of－Danio－reriro
ObjectiveThe objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation
Whole－mount－fluores...
实验概要The method provides a protocol for whole mount fluorescent immunohistochemistry. The advantage of using fluorescence to stain whole mount sectio
Dorsal－Lip－Transplant－in－Amphibian－Embryos
IntroductionIn early stages of development in amphibian embryos, very few cells' fates are rigidly determined. However, previous experiments show
Fate－Mapping－Axolotl－Embryos－in－Early－Gastrulation
BackgroundSince different amphibians execute gastrulation in different ways, the study of amphibian gastrulation has been complex. A popular method us
Exercise－12.10－－Establishment－of－a－Primary－Culture
Exercise 12.10 - Establishment of a Primary CultureLEVEL IIIMaterialsChick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scis