HowtomakeDEPCtreatedwaterandTrisBuffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at 37°C.Autoclave for 15 minutes to remove any trace of DEPC.Notes:How to make DEPC-treated Tris buffers: DEPC will react with primary amines and cannot be used directly to treat Tris buffers. DEPC is highly unstable in the presence of Tris buffers and deco......阅读全文
How－to－make－DEPCtreated－water－and－Tris－Buffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at
How－to－Make－Simple－Solutions－and－Dilutions
1. Simple Dilution (Dilution Factor Method based on ratios)A simple dilution is one in which a unit volume of a liquid material of interest is combine
Oligo(dT)纤维素层析分离Poly(A)+－RNA
Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose ChromatographyJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, Austra
Tris缓冲液（TrisHCl－buffer）的配制
将121g的Tris碱溶解于约0.9L水中，再根据所要求的pH（25℃下）加一定量的浓盐酸（11.6N），用水调整终体积至1L。浓盐酸的体积（ml）pH8.6142128.53846566671.3769.08.88.68.48.28.07.87.67.47.2
Protein－Expression－and－Purification－Protocol
Step 1: Transform appropriate DNA plasmid into BL21(DE3) E. coli cells. These cells must be competent. (Protocol for how to make competent cells.)a) T
RNase－and－DEPC－Treatment:－Fact－or－Laboratory－Myth
Researchers are usually trained in RNA isolation and analysis methods by one another or by technical manuals. Experimental procedures are often not qu
2－Dimensional－Gel－Electrophoretic－Analysis－for－Chicken－Egg
Overview This protocol is a detail description of the procedure in performing 2D gel electrophoresis for illustrating the protein profile of the w
E.Z.N.A.®－Total－RNA－Midi－Kit－Protocol－DNase－I－digestion－Protocol
实验概要E.Z.N.A.® Total RNA Midiprep Kit provides a rapid and easy method for the isolation of up to 600 ug of total RNA from cultured eukaryotic cells,
E.Z.N.Z.TM－MicroElute－RNA－Desalting－and－Concentration－protocol
实验概要This protocol is designed to clean up and concentrate RNA from various sources such as RNA isolated with RNA-solv® Reagent and other phenol involv
Extraction－of－RNA－from－Fibrous－tissues
实验概要E.Z.N.A.™ MicroElute® Total RNA Kit provides a rapid and easy method for the isolation of up to 50 ug of total RNA from small amount of cultured
E.Z.N.Z.TM－MicroElute－RNA－Cleanup－Protocol
实验概要This protocol is designed to recovery RNA from enzymatic reactions such as DNase I digestion, In vitro transcription, etc. For RNA desalting o
E.Z.N.A.®－Total－RNA－Midi－Kit－Protocol－for－Eukaryotic－Cells－and－Tissues
实验概要E.Z.N.A.® Total RNA Midiprep Kit provides a rapid and easy method for the isolation of up to 600 ug of total RNA from cultured eukaryotic cells,
Polygalacturonase－assay
This enzyme is famous for being involved in the development of the GMO tomatoes (more information from the link at the foot of this page). The cells o
Isolation－of－Nonmuscle－Actin
General Preparation1. Prepare buffers and have them cold. Make 50X stock of Buffer G and dilute as needed from frozen aliquots of 50X Buffer G.1X Extr
蛋白质电泳技术
Serum Protein Electrophoresis Tricine/Polyacrylamide Gel ElectrophoresisUsed for pilin processing analysis but generally useful for resolution of smal
Two－Dimensional－Gel－Electrophoretic－Analysis－for－the－Human－Plasma－Proteome
OverviewThis protocol is a detail description of the laboratory procedure in performing 2D gel electrophoresis for illustrating the protein profile of
常用试剂配制－5
Sodium citrate (MW 294.10)0.09 MDissolve 2.65 grams of sodium citrate to a final concentration of 100 ml with water.Sodium citrate/formaldehyde (for s
Isolation－and－Quantification－of－Genomic－DNA－from－Mycobacterium－tuberculosis
Part A. Isolation of Nucleic AcidsNOTE: CAUTION! STEPS 1-10 SHOULD BE PERFORMED USING APPROPRIATE PROCEDURES FOR HANDLING MATERIAL POTENTIALLY CONTAMI
Differential－Display－of－Cotton－Transcripts
Plant MaterialsCotton ovules (Gossypium hirsutum cv. Coker 312) were collected 8, 15, and 20 days after anthesis. Total RNA was extracted from strippe
In－vitro－Sphingomyelinase－Assay
Reagents:Lysis buffer25 mM Tris-HCl, pH 7.45 mM EDTA1 mM ATP20 µg/ml CLAP1 mM PMSFBuffer A10 mM MgCl20.2 M Tris-HCl, pH 7.40.2 % Triton X-100Buffer B0
基本实验技术
I. Safety ProceduresA. ChemicalsA number of chemicals used in this laboratory are hazardous. All manufacturers of hazardous materials are required by
来自耶鲁大学的PCR常见问题的精辟总结
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles. COMPONENTVOLUMEFINAL CON
RNA－Isolation－From－Animal－tissue－or－cell－culture
实验概要This method is designed for most animal tissues and culture cells. For RNA isolation from fibrous tissue, follow the specialized protocol on pag
Lipoprotein－Analysis－Week－2:－Electrophoresis2
Preparation of stacking gelPrepare a 7.5 ml of 3% stacking gel in a small beaker using the following amounts of appropriate reagents.Stockfinal conc.A
DNA－isolation－extraction
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation
Isolation－of－Genomic－DNA－from－Tissue－Using－ChargeSwitch®－Technology
实验概要 The ChargeSwitch® gDNA Mini and Micro Tissue Kits allow rapid and efficient purification of genomic DNA from mini (10-25 mg) or micro (3-5 mg)
E.Z.N.A.®－Total－RNA－Maxi－Kit－Protocol－for－Animal－Tissues
实验概要The E.Z.N.A.® Total RNA Maxi Kit uses HiBind® matrix spin-column technology to isolate up to 5 mg total cellular RNA from a variety of sources
RNA实验方法
Solublization of RNA in Formamidecontributed by James McCaughern-Carucci, Yale UniversityResuspending RNA in Formamide (as reported by Chomczynski et
Stripping－for－repro...
实验概要The following protocol provides a method of removal of antibodies from western blots.实验原理Stripping is the term used to describe the removal of pr
从棉花组织中提取微量RNA的标准实验方法protocol
Prior to Extractions ・ Bake all necessary glassware, metal spatulas, mortars, and pestles overnight in a 200℃ oven after wrapping them in aluminum f