AutoradiographyandIntensifyingScreens
Autoradiography and Intensifying ScreensFrom time to time, someone asks a question about intensfying screens, both in my lab and in these web forums (like why do you put them in the deep freeze?). Recently, John Attwood of Kodak submitted the following explanation about how they work, and gave permission to post it here as a general "protocol".Intensifying screens are used to reduce the exposure time or inc......阅读全文
Autoradiography－and－Intensifying－Screens
Autoradiography and Intensifying ScreensFrom time to time, someone asks a question about intensfying screens, both in my lab and in these web forums (
Autoradiography
MaterialsH-Thymidine, specific activity of 2.0curie/millimoleOnion sets, jars and toothpicksAlcohol-acetic acid fixativeMaterials for feulgen reaction
Autoradiography－(35S)
Remove Kodak NTB2 nuclear emulsion from fridge and place at 42oC for around 30-60 mins (until melted).Make up the developer and the fixer and place in
Standard－Protocols－Autoradiography－(35S)
Remove Kodak NTB2 nuclear emulsion from fridge and place at 42oC for around 30-60 mins (until melted).Make up the developer and the fixer and place in
Exposing－gels－and－plates－containing－radioactive－samples－to－Xray－film
Although most people use the PhosphorImager for western blots, kinase assays and methionine-labeled samples, X-ray film remains the best way to expose
HISTONE－KINASE－ASSAY
PROTOCOLTo 1.5 mL eppendorf tubes add:200 µg of protein extract (see Western blot protocol for protein sample preps)q.s. to 300 µL with RIPA (with pro
Chemical－Genomics－Approaches－in－Plant－Biology
Chemical genomics (i.e., genomics-scale chemical genetics) approaches are based on the ability of low-molecular-mass molecules to modify biologica
Differential－Display－of－Cotton－Transcripts
Plant MaterialsCotton ovules (Gossypium hirsutum cv. Coker 312) were collected 8, 15, and 20 days after anthesis. Total RNA was extracted from strippe
RLGS－protocol
A. Preparation of DNA SolutionIn the case of rice, for example This method may be appllicable for many grass species and some other plants.
Nature新技术：CRISPR+单细胞测序=？
CRISPR-Cas9“基因剪刀”的基因组编辑技术是生物研究和新型靶向药物研发的有力工具。比如利用CRISPR筛选基因（pooled CRISPR screens），可以通过CRISPR gRNAs靶向成百上千个不同的基因，同时编辑许多细胞，然后实验筛选编辑细胞，gRNA可以帮助确定哪些基因对于生物
细胞培养——细胞生长和细胞毒性
Articles posted in the Method Froum Cell Viability AssayDye exclusion method Viable Cell Counts Using Trypan Blue (Gibco) Soft Agar Assay For Colo
放射自显影[术]的技术特点
中文名称放射自显影[术]英文名称autoradiography;radioautography定 义利用放射性同位素所产生的电离辐射对感光乳胶的氯化银晶体而产生潜影，再经过显影定影处理，把感光的氯化银还原成黑色的银颗粒，即可根据这些银颗粒的部位和数量分析出标本中放射性示踪物的分布，以进行定位和定量
放射自显影[术]
中文名称放射自显影[术]英文名称autoradiography;radioautography定 义利用放射性同位素所产生的电离辐射对感光乳胶的氯化银晶体而产生潜影，再经过显影定影处理，把感光的氯化银还原成黑色的银颗粒，即可根据这些银颗粒的部位和数量分析出标本中放射性示踪物的分布，以进行定位和定量
放射自显影技术的概述
放射自显影技术是利用放射性同位素的电离辐射对乳胶（含AgBr或AgCl）的感光作用，对细胞内生物大分子进行定性、定位与半定量研究的一种细胞化学技术。放射自显影技术（radioautography；autoradiography）用于研究标记化合物在机体、组织和细胞中的分布、定位、排出以及合成、更
Quantitative－PCR
实验概要Quantitative PCR involves co-amplification of two templates: a constant amount of a preparation containing the desired target sequence and var
全细胞靶点筛选抗生素新药的方法
A target-specific whole cell assay for antibacterial drug discoveryLorraine HernandezSrinivas KodaliDoris CullySheo SinghJun Wang , jun_wang2@merck.co
定量PCR实验技术－QPCR
Quantitative PCRJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid W. RussellUniversity of Texas Southwes
Alkaline－agarose－gel－electrophoresis
Alkaline agarose gel electrophoresis (Sambrook et al., 1989)Alkaline agarose gels can be used to determine the size and quality of first and second st
凝胶渗透色谱中专业英语
死时间 t0，dead time保留时间 tR，retentiontime调整保留时间 t''R，adjustedretention time死体积 V0，dead volume保留体积 vR，retentionvolume调整保留体积 v''R，adjustedre
凝胶渗透色谱中专业英语
死时间 t0，dead time保留时间 tR，retentiontime调整保留时间 t''R，adjustedretention time死体积 V0，dead volume保留体积 vR，retentionvolume调整保留体积 v''R，adjustedre
酵母双杂交系统
· Yeast Two-Hybrid System (Finley Lab)This is one of the most comprehensive and detailed guide to yeast two-hybrid system technique with intro
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Sleeping Beauty transposon mutagenesis in rat spermatogonial stem cellsZoltán Ivics,1, 2 Zsuzsanna Izsvák,1, 2 Gerardo Medrano,3, 4 Karen M Chapman3,
新的基因组工具组合或可梳理疾病的因果关系
近日，奥地利科学院分子医学研究中心（CeMM）的研究人员Christoph Bock在基因组生物学会议上发言称，新的基因组工具组合可以帮助研究人员梳理疾病的因果关系。 他认为，单细胞RNA-seq和ATAC-seq等方法可以帮助人们深入了解疾病发展过程中发生的情况，但它们无法建立因果关系。Bo
什么是放射自显影术？
免疫放射自显影术（autoradiograph)旨在追踪某些物质在体内、组织或细胞中的分布与代谢径路。首先，将放射性同位素或放射性同位素的标记物注入动物体内或加入培养基中，间隔一定时间取材，制成标本（如切片），在暗室中于标本的上面涂以液体原子核乳胶，置暗处曝光，数日后再经显影和定影处理，或经染色
cDNA－LIBRARY－SCREENING
PREPARE SOLUTIONS1. 10mM MgSO4, 0.2% Maltose LB (100 mL):Mix 1.0 g of Bacto-Tryptone, 1.0 g of NaCl, 0.5 g of Yeast Extract, and 1.0 mL of 1M MgSO4. A
科学家拓展CRISPRCas9系统在癌症治疗中的应用
近日，英国剑桥大学等科研机构的科研人员在Nature上发表了题为“Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens”的文章，研究人员基于功能基因组学，利用CRISPR-Cas9系统对多种癌症治疗的潜在靶标
AA－－Metabolite－Quantitation－of－Media－PostAA－Labeling
1) Remove 2 500 µl aliquots of supernatant into scintillation vials, add scintillation fluid and count.2) Aliquot 1.6 mls of the remaining supernatant
Analysis－of－Proteins－using－Small－Format－2D－Gel－Electrophoresis
Preparation of protein samplesIntracellular virus proteinsThe following method has been developed principally for the analysis of intracellular protei
酵母转化
· Yeast Transformation (Gietz Lab)LiAc/SS-DNA/PEG Transformation· Yeast Transformation (Breeden Lab)LiAc method· Large-Scale Y
CRISPRRNAseq:把混乱的基因编辑结果屡清楚
对复杂基因网络的分析可以简单概括为三步：1.向大量细胞引入CRISPR基因编辑系统。2.应用单细胞RNA测序分析这些基因编辑的影响。3.分析基因型和表型之间相互作用的遗传途径。通过遵循这些步骤，研究人员可以比以前更有效地探索基因组遗传风险因子的功能影响，观察和研究癌细胞中发生突变的具体基因。