InPlantaTransformationofArabidopsis
实验概要 A breakthrough in Arabidopsis research was the invention ofthe vacuum-infiltration procedure, a simple and reliable methodof obtaining transformants at high efficiency while avoidingthe use of tissue culture. The plant transformation proceduresdescribed here involve floral dip, vacuum infiltration, andspraying. They yield transformants at frequencies ranging upto several pe......阅读全文
Arabidopsis－gDNA－isolation
This is a simple and fast protocol for the extraction of genomic DNA from Arabidopsis thaliana that works fine in PCR for simple amplicons. We only us
In－Planta－Transformation－of－Arabidopsis
实验概要 A breakthrough in Arabidopsis research was the invention ofthe vacuum-infiltration procedure, a simple and reliable methodof obtaining
Transformation－Protocol－for－Arabidopsis
Transformation Protocol for Arabidopsis – AbbreviatedGerminate seed in pots↓ 4 weeksStreak bacteria onto YM/MinA↓ 2-3 days 28°CSpray/dip bacterial sus
Simplified－Arabidopsis－Transformation－Protocol
(Brief version for those who are familiar with the method)Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign.Our present proto
Arabidopsis－RNA－extraction－protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below). Spin
Cell－Suspension－Culture－of－Arabidopsis
实验概要Cell Suspension Culture of Arabidopsis 主要试剂10% (v/v) Household BleachCallus Induction Medium Gamborg's B5 Basal Medium 0.5 g/liter M
Simplified－Arabidopsis－Transformation－Protocol
实验概要Our present protocol (Clough and Bent, 1998; modified from Bechtold et al. 1993) is extremely simple. We have found that the MS salts, hormone
Arabidopsis－RNA－extraction－protocol
1-2 g fresh material, freezer-dried, ground with 0.2g sand (if necessary), and then homogenized with 10ml RNA extraction buffer (see below).Spin at 8,
Production－of－Antibody－Fragments－in－Arabidopsis－Seeds
Plants offer a number of attractive benefits over conventional mammalian or bacterial cell culture systems for the production of valuable pharmace
ChIP－using－plant－samples－–－Arabidopsis
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
Adventitious－Root－Induction－in－Arabidopsis－thaliana－as－a－Model－for－In...
Adventitious root formation, the development of roots on non-root tissue (e.g. leaves, hypocotyls and stems) is a critical step during micropropag
Studying－Arabidopsis－Envelope－Protein－Localization－and－Topology－Using－...
Chloroplasts are metabolically important organelles that perform many essential functions within plant cells. The chloroplasts can be subdivided i
Green－lab－protocol－for－vacuum－infiltration－transformation－of－Arabidopsis
This protocol is adapted from protocols by Nicole Bechtold (Bechtold et al. 1993), Andrew Bent (Bent et al. 1994) and Takashi Araki. No claims are
植物抗体系列及分类
PHYTOAB抗体产品主要包括以下：PRIMARY ANTIBODIES for:叶绿体相关抗体线粒体相关抗体细胞质相关抗体质膜相关抗体高尔基体相关抗体内质网相关抗体质体相关抗体过氧化物酶体相关抗体细胞核相关抗体液泡相关抗体细胞壁相关抗体转录因子相关抗体标签抗体内参抗体SECONDARY ANTIB
光系统Ⅰ抗体简介及分类
PHYTOAB公司最新研发了许多用于光合作用研究的抗体。并且有些抗体是蛋白A纯化或免疫亲和纯化的兔多克隆抗体，可以广泛应用于Western blot , ELISA等。 光合作用相关抗体 PSIPSI的主要亚基包括PsaA和PsaB，是光系统I的密切相关蛋白，参与P700，A0 (chlorophy
[科学]：研究人员破译植物开花时间秘密
植物为什么会在不同季节开花?研究人员在新一期《科学》杂志上报告说，其秘密在于一种核糖核酸(RNA)起到了调控作用。 英国约翰·英尼斯中心的研究人员发现的这种核糖核酸名为COOLAIR，是一种反义长链非编码核糖核酸。长链非编码核糖核酸曾被认为没有功用，现在研究人员发现它能发挥很多重要的功能，
Global－Expression－Profiling－of－RNA－from－Laser－Microdissected－Cells－at－...
Global expression profiling of RNA isolated from laser microdissected cells allows one to profile a specific set of cells allowing for enhanced se
DoseResponse－to－and－Systemic－Movement－of－Dexamethasone－in－the－GVGInducible
DoseResponse to and Systemic Movement of Dexamethasone in the GVG-Inducible Transgene System in ArabidopsisConstruction of transgenic plants is centra
Preparation－of－Stroma,－Thylakoid－Membrane,－and－Lumen－Fractions－from－...
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic AnalysisFor many studies regarding
GenomeWide－Identification－of－Transcription－FactorBinding－Sites－in...
Genome-Wide Identification of Transcription Factor-Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip)
亚细胞定位用荧光显微镜能看到吗
目前进行亚细胞定位研究，提取拟南芥(Arabidopsis thaliana)(Arabidopsis thaliana)叶肉原生质体进行PEG转化。研究目标采用GFP/YFP融合蛋白，但是在LSM710观察时，叶绿体有很明显的背景，尽管有些细胞是明显看到了GFP荧光，远远强于叶绿体背景，但是拍的照
Chemical－Genomics－Approaches－in－Plant－Biology
Chemical genomics (i.e., genomics-scale chemical genetics) approaches are based on the ability of low-molecular-mass molecules to modify biologica
细胞质膜H+ATPase抗体在植物研究的多种应用
植物细胞膜H+-ATPase，广泛分布于植物细胞质膜上的ATP酶，由Hodges等人于1972年研究质膜微囊的ATPase的水解活力时发现。根据功能和位置不同，植物细胞内的H+-ATPase可分为F、P和V三大类型：F型ATPase主要分布在线粒体和叶绿体上；P型ATPase分布在质膜上；V型ATP
IMAGINGPAM相关文献集（一）
德国WALZ公司生产的M系列调制叶绿素荧光成像系统IMAGING-PAM，作为全球最权威的叶绿素荧光成像系统，在国际科研界得到了广泛应用，已经成为叶绿素荧光成像研究的行业标准。 泽泉科技有限公司作为德国WALZ在中国的技术服务中心，一直以来致力于推广调制叶绿素荧光技术在国内科研界的应用，并
IMAGINGPAM相关文献集（四）
151. 邓培雁, 刘威, 韩博平. 宝山堇菜（Viola baoshanensis）镉胁迫下的光合作用 生态学报,2007, 27(5):1858-1862. 152. 邓培雁, 刘威, 韩博平, 韩志国. 宝山堇菜（Viola baoshanensis）、紫花地丁(V. y
最常见的模式生物
最常见的模式生物有：逆转录病毒（retrovirus），大肠杆菌（Escherichiacoli），酵母（buddingyeast（Saccharomycescerevisiae），fissionyeast（Schizosaccharomyces pombe）），秀丽线虫（Caenorhabditi
植物细胞质雄性不育育性恢复机理研究获进展
植物细胞质雄性不育(CMS)及育性恢复调控网络受控于细胞质和细胞核两套系统，是研究质核互作的模式系统。由于模式植物拟南芥 (Arabidopsis thaliana)未发现天然CMS，研究人员不得不利用其它遗传系统探索这一重要遗传机制。萝卜具有大量的天然CMS，与拟南芥 (Arabidops
版纳植物园转录调控因子Alfinlike基因家族研究获进展
通过比较基因组学，在基因组层次上研究特定基因家族的进化，进而揭示其变化的原因和机制，已成为目前进化生物学关注的热点问题。中科院西双版纳热带植物园生态进化生物学研究组研究人员利用模式植物拟南芥(Arabidopsis thaliana)及其近缘物种琴叶拟南芥(A. lyrata)和小盐芥(Th
IMAGINGPAM相关文献集（三）
101. Li Q-M, Liu B-B, Wu Y, Zou Z-R. Interactive Effects of Drought Stresses and Elevated CO2 Concentration on Photochemistry Efficiency of C
拟南芥转录复合物参与调控植物盐害反应机制
在自然界中植物的生长发育往往受到各种环境胁迫(Environmental stresses)的影响，如高温、低温及干旱等。其中土壤的盐碱化(Salinity stress)是限制农作物栽培及产量的重要环境因子，但是人们对植物耐盐害的潜在分子机制仍不十分清楚。WRKY家族是一类植物特有的转