GuidelinesforAsepticRodentSurvivalSurgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help ensure optimal results for aseptic survival surgery using laboratory rodents.Materials::Note: A sterile instrument pack should not be used on more than five individual rodents.Surgical Area -benchcoat paper or other clean covering cold disinfectantSterilization......阅读全文
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
Yale－University－Policy－for－Aseptic－Amphibian－Survival－Surgery
Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the Guid
Experimental－Surgery
General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa. Experimental surgery has
基本无菌化技术
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
Principles－of－Aseptic－Technique
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
基本无菌化技术4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Experimental－Surgery2
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or wh
Principles－of－Aseptic－Technique——4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Aseptic－Techniques
Aseptic techniques ensure that all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi, mycoplasm
Experimental－Surgery3
Antibiotic GuidelinesGeneral RecommendationsSelectionIf a culture and sensitivity is not available, select antibiotics based on probable organism and
Isolation－of－rodent－pancreatic－β－cells
1. Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2. Rat islets were isolated from male wistar
B－Cell－Survival－Pathway
Physical interactions between intergrin alpha4beta1 heterodimer expressed on B cells and counter receptors on stroma cells are key mediators of the su
Cell－Clonogenic－Survival－Assay
DescriptionAllows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Procedure1. Grow
siRNA－Design－Guidelines
Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA, such as chemical synthe
Asepsis－and－Aseptic－Practices－in－the－Operating－Room——2
Principle #4All items introduced onto a sterile field should be opened, dispensed, and transferred by methods that maintain sterility and integrity.2,
Rodent－Retinal－Ganglion－Cell－Cultures
实验概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Principles－of－Aseptic－Technique——2
PersonnelAseptic technique requires careful attention to a series of steps which begins with patient and instrument preparation and ends at final woun
ELISA－Protocol－(General－Guidelines)
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known
ELISA－Protocol－(General－Guidelines)
实验概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples containing known
Long－PCR－Reagents－and－Guidelines
George Church Lab, Harvard Medical SchoolPCR_protocol.html">http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.htmlEfficient Long PCR results fr
Asepsis－and－Aseptic－Practices－in－the－Operating－Room
Preventing surgical site infection in the operating room is the primary goal of the surgical team, and all activities performed by the team support th
Opposing－roles－of－AIF－in－Apoptosis－and－Cell－Survival
Programmed cell death is induced by many different factors and involves numerous signaling pathways, some dependent on caspase proteases and others th
Role－of－Erk5－in－Neuronal－Survival
Axons extend significant distances to innervate target tissues. At the site of innervation, target tissues release neurotrophins including NGF, BDNF a
LongPCR－Reagents－and－Guidelines
Long-PCR Reagents and Guidelinesfrom George Church as Modified from Cheng et al. (1)General Guidelines for Long-PCR Conditions and Enzyme Mixtures====
Aseptic－Technique－and－Good－Cell－Culture－Practice
AimTo ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross con
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amn
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells3
Moreover, when the method was applied to the analysis of the cellular composition of immature testes, the results were also in agreement with previous
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells2
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensio