YaleUniversityPolicyforAsepticAmphibianSurvivalSurgery
Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the Guide for the Care and Use of Laboratory Animals, all survival surgery should be performed using aseptic procedures, including the use of sterile surgical gloves, masks, sterile instruments, and aseptic techniques.Policy:Aseptic technique must be applied to all su......阅读全文
Yale－University－Policy－for－Aseptic－Amphibian－Survival－Surgery
Background: Aseptic surgery by definition is surgery performed without contamination or exposure to pathogens. According to the AWR's and the Guid
Guidelines－for－Aseptic－Rodent－Survival－Surgery
Introduction:Aseptic surgery is surgery performed without contamination or exposure to pathogens. These policies and guidelines are provided to help e
基本无菌化技术
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
Principles－of－Aseptic－Technique
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
基本无菌化技术4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Dorsal－Lip－Transplant－in－Amphibian－Embryos
IntroductionIn early stages of development in amphibian embryos, very few cells' fates are rigidly determined. However, previous experiments show
Yale－Immunofluorescence－Protocol
实验概要We provide a protocol for fixation, immunostaining, and imaging in 384-well Plates.主要试剂Reagents1. 384-well view plates (Aurora)2. HUVEC (pooled, L
Experimental－Surgery
General Issues and RequirementsSurgery is defined as any procedure that exposes tissues normally covered by skin or mucosa. Experimental surgery has
Principles－of－Aseptic－Technique——4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Aseptic－Techniques
Aseptic techniques ensure that all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi, mycoplasm
Experimental－Surgery2
Use of Expired MaterialsExpired medical materials such as drugs, fluids and sutures may not be used on any research animal who is unanesthetized or wh
Experimental－Surgery3
Antibiotic GuidelinesGeneral RecommendationsSelectionIf a culture and sensitivity is not available, select antibiotics based on probable organism and
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal2
GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia o
B－Cell－Survival－Pathway
Physical interactions between intergrin alpha4beta1 heterodimer expressed on B cells and counter receptors on stroma cells are key mediators of the su
Cell－Clonogenic－Survival－Assay
DescriptionAllows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Procedure1. Grow
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Principles－of－Aseptic－Technique——2
PersonnelAseptic technique requires careful attention to a series of steps which begins with patient and instrument preparation and ends at final woun
今日直播|耶鲁大学副教授讲述可变形软体机器人
原文地址：http://news.sciencenet.cn/htmlnews/2023/7/505639.shtm 直播时间：2023年7月28日（周五）20:00-21:30 直播平台： 科学网APP （科学网微博直播间链接） 科学网微博 科学网视
Role－of－Erk5－in－Neuronal－Survival
Axons extend significant distances to innervate target tissues. At the site of innervation, target tissues release neurotrophins including NGF, BDNF a
Opposing－roles－of－AIF－in－Apoptosis－and－Cell－Survival
Programmed cell death is induced by many different factors and involves numerous signaling pathways, some dependent on caspase proteases and others th
戊二醛固定细胞【Harvard－University】
Yu-Li Wang Lab,University of massachusetts Medical School(UMASS)http://ylwang.umassmed.edu/protocol/cfs/glutar.htmMaterials
Asepsis－and－Aseptic－Practices－in－the－Operating－Room
Preventing surgical site infection in the operating room is the primary goal of the surgical team, and all activities performed by the team support th
Transplantation－of－theeyeforming－region－of－Amphibian－neurula－into－theflank
EMBRYO CELL AND TISSUE CULTURE TECHNIQUESRearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solution.Micro
Aseptic－Technique－and－Good－Cell－Culture－Practice
AimTo ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross con
Asepsis－and－Aseptic－Practices－in－the－Operating－Room——2
Principle #4All items introduced onto a sterile field should be opened, dispensed, and transferred by methods that maintain sterility and integrity.2,
Developmental－and－phenotypic－implications－of－transplantation
ObjectiveIn this experiment, a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the
JAMA－Surgery：减肥术治疗2型糖尿病
近日，发表在《JAMA Surgery》的一项研究表明， Annemarie G. Hirsch博士和他的同事们研究了DiaRem得分（经过验证的分数是来自比较易得的数据），DiaRem得分可以用于预测经过减肥手术的病人是否会促使2型糖尿病治愈。这个分数可以用来预测减肥手术是否会促使短期缓解糖尿
Emory－University：有望揭示系统性红斑狼疮病因
近日，一项刊登于Nature Immunology的研究报告中，来自埃默里大学的科学家们在自身免疫性疾病—系统性红斑狼疮（SLE，systemic lupus erythematosus）研究上取得了重大进展，相关研究结果有望揭示该病的发病原因。 在系统性红斑狼疮患者机体中，免疫系统B细胞会处
Development－of－the－Heart－Morphogenetic－Field－in－the－Axolotl－Embryo
IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d