ComparisonofEnzymaticandNonEnzymaticMeans3
MTT Assay on Reattached CellsAs seen in Fig. 2 , the proportion of viable MSC that re-attached was significantly higher (p = 0.0004) upon dissociation with trypsin (82.1% ± 2.0%) compared to enzyme-free dissociation buffer (5.0% ± 0.2%). The same trend was observed after the dissociated MSC were subjected to freeze–thawing (68.4% ± 3.8% versus 2.8% ± 0.4%, respectively, p = 0.002......阅读全文
Comparison－of－Enzymatic－and－NonEnzymatic－Means1
Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem Cells The dissociation of adherent mesenchym
Comparison－of－Enzymatic－and－NonEnzymatic－Means2
MTT Assay on Reattached Cells MSC were seeded in 12-well cell culture dishes with 5.0 × 104 cells per well (≈4.8 cm2). After 5 to 6 days of culture,
Comparison－of－Enzymatic－and－NonEnzymatic－Means3
MTT Assay on Reattached Cells As seen in Fig. 2 , the proportion of viable MSC that re-attached was significantly higher (p = 0.0004) upon dissoci
Comparison－of－lysis－methods
Comparison of lysis methods Sonication most efficient method of cell fractionation problem: heat build up which can denature proteins (proport
ImmunohistochemistyEnzymatic－Protocol
OverviewR&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells2
Flow Cytometric Analysis Prior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspen
Comparison－of－Beta－oxidation－in－mitochondria－and－peroxisomes－andglyoxysomes
In addition to the beta-oxidation of fatty acids in mitochondria, animal cells carry out beta-oxidation in another organelle, the peroxisome. Although
Azalea－Phylogeny－Reconstructed－by－Means－of－Molecular－Techniques
Plants belonging to the Rhododendron subgenera Pentanthera (deciduous) and Tsutsusi and Azaleastrum (evergreen) are called azaleas. Concerning their m
Biosynthesis－of－Proline－in－Bacteria
Among the twenty amino acids encoded by the genetic code, proline is unique in having a cyclic structure with its side chain connected to the amino gr
镜面增强技术（MEANS）提升超分辨显微效果
【2016中国光学重要成果推荐】北京大学席鹏课题组开发了一种镜面增强的超分辨显微技术，该技术能够将传统的共聚焦系统转变为轴向超分辨系统。同时，这一技术能够大幅提升STED超分辨的分辨率。利用这一技术，研究者首次揭示了细胞核孔和病毒丝的超分辨精细结构。镜子或许是人类最早发明的光学器件。迄今为止，它仍然
酶活性分析（enzymatic－activity－analysis）法检定蛋白质
表现出来的酶GUS可以水解其基质衍生物pNPG,所产生的黄色生成物p-nitrophenol,可供活性测定 （Jefferson et al, 1986）；仪器用具：ELISA光度计 （Dynatech）；微量滴定盘 （microtiter plate, Nunc F96 Maxisorb 4424
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Purification of PCR Products in Preparation for CloningJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne, AustraliaDavid
欧盟评估来自一种米曲霉的α淀粉酶的安全性
2019年11月27日，欧盟食品安全局就来自米曲霉（Aspergillus oryzae）菌株DP‐Bzb41的α-淀粉酶（α-amylase）的安全性评价发布意见。 据了解，该种食品酶是由美国公司Danisco US Inc.使用米曲霉菌株DP‐Bzb41生产的，旨在用于食品烘焙和酿造工艺、
a－pipeline－for－the－identification－of－intact－Nglycopeptides（六）
Figure 4. Comparison of different decoy strategies. The estimated FDR of each decoy method is compared with the real FDR. “Decoy: + 11” m
led光源的术语（一）
Image(Lighting)Contrast The ratio of difference between light and dark as shown on a screen. The change of contrast level refers to 'tone'.
昆明植物所揭示吡啶环生物合成新机制
生物碱是存在于自然界有机体中的一类含有负氧化态氮原子的环状化合物，其数量众多，结构复杂，大多具有广泛的生物活性，由其开发的药物约占全部植物药的40%以上。有关生物碱的研究是目前天然药物化学领域的重点和热点。吡啶生物碱作为生物碱的一类，其中的吡啶环是其发挥药效活性的关键基团。前人的研究表明大部分吡
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Thermo Fisher 'Going Directly After' $250M Q-TOF Market with Release of New Q Exactive Mass SpecDENVER — With the release of its new Q Exactive mass s
Protocol－for－competitive－RTPCR
For quantifying mRNA, we use a competitive RT-PCR protocol with internal standard RNAs. These are added in a defined quantity to the RNA sample prior
siRNAs结合生物芯片的实验设计1
Ambion and Applied Biosystems have joined forces to provide a complete convenient, solution for performing gene silencing experiments and validating
丁显廷/林关宁团队开发新型质谱数据聚类方法比较框架
随着大规模细胞术在医学研究中的应用越来越广泛，已经开发出了多种聚类方法，包括半监督和无监督的聚类方法，用于数据分析.选择最优的聚类方法可以加速有意义细胞群体的识别。 2019年12月23号，上海交通大学丁显廷、林关宁团队在Genome Biology上在线发表了题为A comparison f
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糖化血红蛋白
糖化血红蛋白(hba1c)是糖尿病患者长期血糖控制的重要监测指标，与糖尿病慢性并发症的发生及发展有着密切联系，是目前为止评价糖尿病患者血糖控制的“金标准”。因此，hba1c控制水平直接影响到糖尿病患者的长期预后。 许多大型的临床研究如dcct(糖尿病控制与慢性并
酶法分析技术在食品行业的应用（二）
ICUMSA (国际糖制品标准方法委员会)ICUMSA method GS 2/3-35 精制糖制品中亚硫酸盐的测定(酶法)ICUMSA method GS 8/1/2/3/4-19 总α-半乳糖酶及蜜三糖的测定(酶法)ICUMSA method GS 8/4/6-4 甜菜汁中葡萄糖及果糖的测定(酶
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells1
Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the
E.－Immunohistochemi...
实验概要We provide a guideline procedure and tips for staining of paraffin embedded sections, including antigen retrieval, chromogenic detection and flu
MSD电化学发光技术定量生物工艺中的杂质
Implementation of electrochemiluminescence technology for quantification of bioprocess impurities使用电化学发光技术ECL定量生物工艺中的杂质 发表时间：2008年作者单位：乌普萨拉大学（瑞典语为Upps
Immunoprecipitation
Immunoprecipitation (IP) is one of the most widely used immunochemical techniques. Immunoprecipitation followed by SDS-PAGE and immunoblotting, is rou