UseoftheB.D.FACSorCaliburFlowCytometers2
Gating:Gating will allow you to view cells of interest by any combination of criteria that you choose. Gating does not change the intensity value assigned to an event as is the case for changes in voltage or compensation. It simply lets you decide which data to view and which data to ignore or discard. It is important to check that small changes in your gates don't have significant effects on your results or else......阅读全文
RNA－Isolation－From－Animal－tissue－or－cell－culture
实验概要This method is designed for most animal tissues and culture cells. For RNA isolation from fibrous tissue, follow the specialized protocol on pag
LIVE/DEAD®－Fixable－Dead－Cell－Stain－Kits
实验概要The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are
流式应用精选——微核检测（体内体外）
注：点击文章名称查看详情。文章名称样本仪器型号备注Assessment of the Genotoxic Potential of Azidothymidine in the Comet,Micronucleus, and Pig-a AssayBone Marrow and Peripheral
Aseptic－Techniques
Aseptic techniques ensure that all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi, mycoplasm
specific－immunodetection－of－cyclins－using－488/630－dual－laser－flow－cytometry
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometryWilliam Telford Hospital for Special SurgeryThis protocol is f
细胞培养实验的无菌化技术
The use of aseptic technique is essential for avoiding the production of infection whilst undertaking tissue culture activities.Many activities take p
COLUMN－MANAGEMENT－色谱柱管理
10.1. Columns used in chromatography should be appropriate for their intended use.色谱所用柱子应适合其既定用途。10.2. Columns should be purchased from approved supp
流式细胞仪检测技术操作方法
一、 细胞和试剂的准备 1. 细胞样品：来源淋巴细胞或外周血的白细胞。 2. 荧光标记单克隆抗体：常用的有FITC（fluorescein -isothiocyanate）和PE（phycoerythrin）标记的单克隆抗体。 3. 封闭抗体：抗Fc受体抗体 4. FACS缓冲液： 1
红细胞裂解的实验方法步骤
IntroductionPrior to using lymphoid tissue cell suspensions for flow cytometric analysis and/or for in vitro functional assays, it is recommended to r
Standard－Protocol－For－Up－to－10－ml－Whole－Blood
实验概要The E.Z.N.A.® Blood DNA Maxi Kit is designed for isolation of genomic DNA from up to 25 ml of fresh, whole blood treated with any common anticoa
Streptomyces:Protocols/Spore－Prep
Spore Prep - Inoculating & HarvestingDescription A spore prep is a method of preserving a sporulating strain of Streptomyces. The stock is stored in 2
免疫荧光
Immunofluorescence Technique (Spector Lab)protocol for immunofluorescence on cells Immunofluorescence Protocol (Walter Steffen)Methanol fixationForma
Comparison－of－Enzymatic－and－NonEnzymatic－Means3
MTT Assay on Reattached CellsAs seen in Fig. 2 , the proportion of viable MSC that re-attached was significantly higher (p = 0.0004) upon dissociati
Extraction－of－RNA－from－Fibrous－tissues
实验概要E.Z.N.A.™ MicroElute® Total RNA Kit provides a rapid and easy method for the isolation of up to 50 ug of total RNA from small amount of cultured
Vybrant®－DyeCycle™－Ruby－stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
whats－the－function－of－compressed－air－filter
Compressed air is an essential utility in many industrial applications, including pneumatic tools, packaging, and conveying systems. It is a clean, ef
流式细胞仪的注意事项
1. 减少非特异荧光染色 （1）细胞的活性和状态：流式细胞仪不但可探测细胞表面的荧光，也可探测细胞内的荧光。因此，如果要检测细胞表面的分子，一定要保证细胞的活性，还应尽可能保持细胞静止，通常在4度进行操作。否则，荧光抗体进入死细胞内，会产生非特异结果。 （2）封闭抗体的应用是必不可少的，因为
Cosmid－DNA－Isolation
实验概要Isolation of high yields of highly pure cosmid DNA using PureLink™ HiPure Plasmid Purification Kits.实验原理The PureLink™ HiPure Plasmid Purification
流式细胞仪(Flow－Cytometry):镏金岁月50年
自从50年前诞生至今，流式细胞仪（Flow cytometry）一直并仍然是无以伦比的高通量、高内涵的单细胞分析技术。 2015年11月，是流式细胞仪诞生50周年之时。人们可能会想象，一种如此长时间以前发明的技术应该到今天会是彻底地不同于当年，但是事实不然，它的基础原理与结构几乎没有什么改变，
E.Z.N.Z.TM－MicroElute－RNA－Cleanup－Protocol
实验概要This protocol is designed to recovery RNA from enzymatic reactions such as DNase I digestion, In vitro transcription, etc. For RNA desalting o
Human－DC－Enrichment－Kit
实验概要Enrich untouched Dendritic Cells (DCs) by depleting T cells, B cells, monocytes/ macrophages, NK cells, erythrocytes and most granulocytes from
Purification－of－Genomic－DNA－Using－PureLink™－Silica－Columns
实验概要The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The kit is designed to efficiently isolate ge
Fungal－Midi－DNA－Kit－Protocol－for－Fresh/Frozen－Specimens
实验概要This protocol is suitable for most fresh or frozen tissue samples allowing more efficient recovery of DNA. However, due to the tremendous variat
Protocol－for－Isolating－DNA－from－Blood－with－Nucleated－Red－blood－Cells
实验概要DNA isolation from fish or avian blood sample can be difficult because it contains nucleated red blood cells. E.Z.N.A. NRBC Blood DNA Kit is de
Column－Method－for－Lambda－Phage－DNA－Preparation
Purpose:Mini-prep method for lambda phage DNA purification from lysates.Time required:4 hours once the lysate is in handSpecial supplies required:BioR
MATRIXF红外光谱英文技术参数
The MATRIX-F can be operated not only with conventional fiber optic probes and flow cells, but also with contactless measurement heads.Depending o
Viscosity－－Polymeriztion－of－Microtubules
LEVEL IIMaterialsTubulin (Brain extract from Exercise 9.4)GTPATPViscometerWaterbath or incubator at 37° CProcedureCompute the amount of GTP (M.W. 523)
流式细胞仪检测技术
实验方法原理 流式细胞仪的使用一般分为三个步骤。第一，是仪器前阶段，包括试剂的准备，细胞制备、细胞的荧光染色；第二，是流式细胞仪阶段，主要是仪器的操作；第三，是结果分析阶段。实验材料 淋巴细胞外周血的白细胞试剂、试剂盒 FACS缓冲液PBS叠氮钠溶液仪器缓冲液FACS清洁液FACS洗净液仪器、耗材
Vybrant®－DyeCycle™－Violet－Stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
Maxiprep－preparation－of－Plasmid－DNA
实验概要The PureLink™ HiPure Plasmid DNA Maxiprep Kit allows purification of 500–850 μg of high-quality plasmid DNA from 100–200 mL overnight E. coli cu