WIKI资讯
WIKI资讯
Protocol1 调制protein A sapharoseprotein A sapharose 5ml 溶于20ml含0.02%NaN3的PBS离心2000转2分，去上清，在沉淀加0.02%NaN3的PBS，离心后去上清，重复2次，最后沉淀加20ml含0.02%NaN3的PBS，冷藏保存用单抗做一抗时，把protein A sapharose先用抗鼠Ig兔血清处理(每50ulprotein A sapharose用1-5ul血清)。旋转1-2h,混匀后，再离心1-2s去上清后，加PBS，vortex混合，离心去上清，重复二次加含0.02%NaN3的PBS到原来体积，冷存。避免长期保存。2．抗原的检出以下操作全在冰面或4度进行去除细胞培养液，用冷PBS洗一次。加RIPA，溶解后，转移到eppentube中用vortex混匀。RIPA的量：6cm的培养皿加1 ml(蛋白质的量为500mg/ml),组织块用液氮粉碎，在缓冲剂中捣......阅读全文
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T CellsINTRODUCTIONThe enzyme-linked immunospot (ELISPOT) assay
AcO- --- part one ---(AcO)2Ca 1; 1.5MAcOK 1; 2; 3; 4; 5; 6; 7; 8M(AcO)2Mg 1M
实验概要This method is use to extract short RNAs from plant tissue. Some of the variables (e.g. centrifugation speeds×, precipitation
Cytokine BioassaysIntroductionBiological activity of cytokines and their concentrations are commonly measured by cellular proliferation of primary cel
if mouse is found deadif mouse is a newborn or < 1 wk of ageIf mouse is alive or moribund and above one week of ageRoutine dissectionFormalin fixat
RICKMEIERR65/250FL-Z-W-SAE3-R-SO S-NR:330110-8HonsbergCM2K-025HMJenaer Antriebstechnik GmbH17H19-0230-10GK1-52(30)CRE Roeler
实验概要Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells and are toxic to infected cells.
In Vitro T Cell ActivationIntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR).
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
Neutralizing Bioassay ProtocolsIntroductionAntibodies that block binding of cytokines to their specific receptors and neutralize their effects are cri
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1 and Renee A. Reijo PeraCenter for Human Embryonic Stem Cell R
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin &nb
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白，如 G 蛋白偶联受体 (GPCR) ，是无法在最常见的生物制药生产宿主，如：中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主，然而 H
IntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequ
Phosphorylation of SubstratesScott T. Eblen, N. Vinay Kumar, and Michael J. WeberDepartment of Microbiology and Cancer Center, University of Virginia
淋巴细胞杂交瘤单克隆抗体技术 实验方法原理 1975年，Kohler和Milstein发现将小鼠骨
实验方法原理1975年，Kohler和Milstein发现将小鼠骨髓瘤细胞和绵羊红细胞免疫的小鼠脾细胞进行融合，形成的杂交细胞既可产生抗体，又可无限增殖，从而创立了单克隆抗体杂交瘤技术。这一技术上的突破不仅为医学与生物学基础研究开创了新纪元，也为临床疾病的诊、防、治提供了新的工具。 制备单
实验方法原理 1975年，Kohler和Milstein发现将小鼠骨髓瘤细胞和绵羊红细胞免疫的小鼠脾细胞进行融合，形成的杂交细胞既可产生抗体，又可无限增殖，从而创立了单克隆抗体杂交瘤
1975年，Kohler和Milstein发现将小鼠骨髓瘤细胞和绵羊红细胞免疫的小鼠脾细胞进行融合，形成的杂交细胞既可产生抗体，又可无限增殖，从而创立了单克隆抗体杂交瘤技术。这一技术上的突破不仅为医学与生物学基础研究开创了新纪元，也为临床疾病的诊、防、治提供了新的工具。 制备单克隆抗体包括动物免疫
AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genom
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the
Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured CellsAchim Breiling and Valerio OrlandoDulbecco Telethon Institute, Institute of Genet
MaterialsTrypsin (Gibco 25200-023)3T3 Medium: 500 mL DME (Invitrogen) + 50 mL FBS (Hyclone) + 5 mL 100x Pen/Strep2x Freezing Medium: 3T3 Medium
Western 杂交（主要内容如下）Preparing of Protein LysatesWestern BlottingFar Western BlottingSemi Dry BlottingStripping MembranesTrouble Shooting and Others
人胚肾 293 (HEK293) 细胞在重组蛋白表达中是最常见的宿主细胞。 这类细胞能够表达大量的膜蛋白，如 G 蛋白偶联受体 (GPCR) ，是无法在最常见的生物制药生产宿主，如：中国仓鼠卵巢 (CHO) 细胞中作表达。 HEK293 虽然是蛋白表达的极好宿主，然而 H
PVDF膜上蛋白的可逆染色 Western杂交时，为确认蛋白是否转至PVDF膜上，可用下列方法对膜上蛋白进行可逆性染色。1. 氨基黑染色染液：0.5% Amido Black (w/v), 25%
Electrophoresis through agarose or polyacrylamide gels is the standard way to separate, identify and purify nucleic acid fragments. The location of th
实验概要Ideally, one would like to be able to directly phosphorylate substrates in an intact cell. This could potentially be performed by intr
实验概要Central neurons lose the ability for axonal regrowth during development and typically do not regenerate their axons following axotomy once the
现代有机化工、石油炼制等工业的发展为人类提供了越来越多的新型材料、新型产品，但同时也带来了越来越多的有毒有害物质。除了在工业生产中常见的无机气体(如一氧化碳、硫化氢、氮氧化物等)外，毒性更大、危害更大的有机物质也开始引起人们的广泛注意。另外，长时间工作在有机物质(蒸汽、挥发物)的环境中，会对人身造成