TroubleshootingforPCRandmultiplexPCR
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles.COMPONENTVOLUMEFINALCONCENTRATION1.autoclaved ultra-filtered water (pH 7.0)20.7µL-2.10x PCR Buffer*2.5µL1x3.dNTPs mix (25 mM each nucleotide)0.2µL200 µM (each nucleotide)4.primer mix (25 pmoles/µL each primer)0.4µL0.4 µM (each primer)5.Taq DNA polymerase (native enzyme)0.2µL1 Unit/25 µL6.genomi......阅读全文
Troubleshooting for PCR and multiplex PCR
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles.COMPONENTVOLUMEFINALCONCE
多重PCR（Multiplex PCR）
一般PCR仅应用一对引物，通过PCR扩增产生一个核酸片段，主要用于单一致病因子等的鉴定.多重PCR(multiplex PCR)，又称多重引物PCR或复合PCR，它是在同一PCR反应体系里加上二对以上引物，同时扩增出多个核酸片段的PCR反应，其反应原理，反应试剂和操作过程与一般PCR相同. 多
多重PCR（Multiplex PCR）
一般PCR仅应用一对引物，通过PCR扩增产生一个核酸片段，主要用于单一致病因子等的鉴定.多重PCR(multiplex PCR)，又称多重引物PCR或复合PCR，它是在同一PCR反应体系里加上二对以上引物，同时扩增出多个核酸片段的PCR反应，其反应原理，反应试剂和操作过程与一般PCR相同. 多
Authentication of Medicinal Plants by SNP-Based Multiplex PCR
Highly variable intergenic spacer and intron regions from nuclear and cytoplasmic DNA have been used for species identification. Noncoding internal
Multiplex PCR Method to Discriminate Artemisia iwayomogi from Other ...
Some plants in the genus Artemisia have been used for medicinal purposes. Among them, Artemisia iwayomogi , commonly referred to as “Haninjin,” is
来自耶鲁大学的PCR常见问题的精辟总结
Troubleshooting discussion is based on the PCR protocol as described in the table below. All reactions are run for 30 cycles. COMPONENTVOLUMEFINAL CON
PCR实验指导与常见问题分析-1
CONTENTPCR guide: a discussion of the main parameters influencing the outcome of the PCR and multiplex PCR reaction in 16 pages/sections and using ove
PCR实验指导与常见问题分析-6
Non-denaturing PAA gelsTo separate PCR products differing in only a few bp in length (for example, microsatellite markers), 6-10% PAA gels need to be
PCR实验指导与常见问题分析-4
Fig. 25. Multiplex PCR of mixtures A-D comparing PCR programs with 2 (green) and 1 (yellow) minute extension time at 54° C annealing temperature. Comp
PCR实验指导与常见问题分析-7
11. All products in my multiplex reaction are weak. How can I improve the yield? Decrease annealing time in small steps (2º C) Decrease ex