TheCitricAcidCycle
The Krebs cycle, also called the citric acid cycle, is a fundamental metabolic pathway involving eight enzymes essential for energy production through aerobic respiration, and, like glycolysis, arose early in evolution. This pathway is also an important source of biosynthetic building blocks used in gluconeogenesis, amino acid biosynthesis, and fatty acid biosynthesis. The Krebs cycle takes place in mitochondria wher......阅读全文
The－Citric－Acid－Cycle
The Krebs cycle, also called the citric acid cycle, is a fundamental metabolic pathway involving eight enzymes essential for energy production through
Gammaaminobutyric－Acid－Receptor－Life－Cycle
Gamma-amino butyric acid (GABA) is an inhibitory neurotransmitter that acts at synapses through binding to ligand-gated ion channels, the GABA recepto
三羧酸循环的定义
三羧酸循环（tricarboxylic acid cycle，TCA cycle）是需氧生物体内普遍存在的代谢途径，分布在线粒体。 因为在这个循环中几个主要的中间代谢物是含有三个羧基的有机酸，例如柠檬酸（C6），所以叫做三羧酸循环，又称为柠檬酸循环（citric acid cycle）或者是T
什么是三羧酸循环？
三羧酸循环（tricarboxylic acid cycle，TCA cycle）是需氧生物体内普遍存在的代谢途径。原核生物中分布于细胞质，真核生物中分布在线粒体。因为在这个循环中几个主要的中间代谢物是含有三个羧基的有机酸，例如柠檬酸（C6），所以叫做三羧酸循环，又称为柠檬酸循环（citric ac
三羧酸循环的过程和意义
三羧酸循环（tricarboxylic acid cycle，TCA cycle）是需氧生物体内普遍存在的代谢途径。原核生物中分布于细胞质，真核生物中分布在线粒体。因为在这个循环中几个主要的中间代谢物是含有三个羧基的有机酸，例如柠檬酸（C6），所以叫做三羧酸循环，又称为柠檬酸循环（citric ac
柠檬酸循环的基本概念和过程
三羧酸循环（tricarboxylic acid cycle，TCA cycle）是需氧生物体内普遍存在的代谢途径。原核生物中分布于细胞质，真核生物中分布在线粒体。因为在这个循环中几个主要的中间代谢物是含有三个羧基的有机酸，例如柠檬酸（C6），所以叫做三羧酸循环，又称为柠檬酸循环（citric ac
关于柠檬酸循环的基本介绍
三羧酸循环（tricarboxylic acid cycle,TCA cycle）是需氧生物体内普遍存在的代谢途径。原核生物中分布于细胞质,真核生物中分布在线粒体。因为在这个循环中几个主要的中间代谢物是含有三个羧基的有机酸,例如柠檬酸（C6）,所以叫做三羧酸循环,又称为柠檬酸循环（citric
Shuttle－for－transfer－of－acetyl－groups－from－mitochondria－to－the－cytosol
Acetyl-CoA is produced in mitochondria through the metabolism of fatty acids and the oxidation of pyruvate to acetyl-CoA. When ATP is needed, this ace
Catabolic－Pathways－for－Arginine－－Histidine－Glutamate－Glutamine,－and－Proline
Glutamine is converted to glutamate by glutaminase or several other enzymes by the removal of the amide nitrogen. Proline is first converted to a Schi
Catabolic－Pathways－for－Methionine,－Isoleucine,－Threonine－and－Valine
The carbon chains of methionine, isoleucine, threonine and valine are broken down to produce succinyl-CoA, which feeds into the Citric Acid Cycle. Met
植物代谢组学案例分析：大豆幼苗叶片对盐胁迫的响应
植物组学：大豆幼苗叶片对盐胁迫的响应 研究对象：大豆 分析检测平台：GC-TOF／MS （BIOTREE） 期刊：PLoS ONE 影响因子：3.057 发表时间：2016 摘要： Clarification of the metabolic mechanisms underlying
植物代谢组学案例分析：大豆幼苗叶片对盐胁迫的响应
植物组学：大豆幼苗叶片对盐胁迫的响应研究对象：大豆分析检测平台：GC-TOF／MS （BIOTREE）期刊：PLoS ONE影响因子：3.057发表时间：2016摘要：Clarification of the metabolic mechanisms underlying salt stress
关于三羧酸循环的基本介绍
柠檬酸循环（citric acid cycle）：也称为三羧酸循环（tricarboxylic acid cycle，TCA循环，TCA），Krebs循环。是用于将乙酰CoA中的乙酰基氧化成二氧化碳和还原当量的酶促反应的循环系统，该循环的第一步是由乙酰CoA与草酰乙酸缩合形成柠檬酸。反应物乙酰辅
MDH2基因突变与药物因子介绍
苹果酸脱氢酶在柠檬酸循环中利用NAD/NADH辅因子体系催化苹果酸对草酰乙酸的可逆氧化。由该基因编码的蛋白质定位于线粒体，可能在苹果酸-天冬氨酸穿梭中发挥关键作用，在细胞溶胶和线粒体之间的代谢协调中起作用已经发现了一些编码不同亚型的转录变体。[由RefSeq提供，2013年9月]Malate deh
MDH2基因编码功能及结构描述
苹果酸脱氢酶在柠檬酸循环中利用NAD/NADH辅因子体系催化苹果酸对草酰乙酸的可逆氧化。由该基因编码的蛋白质定位于线粒体，可能在苹果酸-天冬氨酸穿梭中发挥关键作用，在细胞溶胶和线粒体之间的代谢协调中起作用已经发现了一些编码不同亚型的转录变体。[由RefSeq提供，2013年9月]Malate deh
DNA－Cell－Cycle
Solutions70% ethanolribonuclease (100 µg/ml DNase free, Sigma)propidium iodide ( 50 µg/ml in PBS)ProcedureHarvest cells. Spin at 1200 rpm for 5 minute
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Neutralizing－Arachidonic－Acid
NOTE - Use arachidonic acid from Biomol (# FA-003)1) Make up the appropriate concentration of AA in 100% ethanol.2) Add 1µl of phenol red solution.-->
Amino－acid－composition
There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi
Arachidonic－Acid－Labeling
1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 time with room temperature PBS.3) Resuspend
Cyclins－and－Cell－Cycle－Regulation
The cell cycle is regulated by the interplay of many molecules. Key among these are the cyclins which are expressed and then degraded in a concerted f
Preparation－of－nucleic－acid－probes
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
Principles－of－nucleic－acid－hybridization
Principles of nucleic acid hybridization5.2.1. Nucleic acid hybridization is a method for identifying closely related nucleic acid molecules within tw
DAPI－Nucleic－Acid－Stain
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
Oxidation－of－Polyunsaturated－Fatty－Acid
Unsaturated fatty acids are those that contain one or more double bonds in their alkyl-chain. Polyunsaturated fats with two double bonds usually have
Protocol－for－Dual－Pulse－Labeling－Using－EdU－and－BrdU－Incorporation
实验概要The measurement of cell proliferation is fundamental to the assessment of cell health, genotoxicity, and drug efficacy. Proliferation is traditi
藤黄果提取物的概述
藤黄果提取物，也称藤黄果抽出物，是植物藤黄果的果皮抽出，精致萃取其有效份HCA(Hydroxy Citric acid；羟基柠檬酸)，含有10–30%类似柠檬酸（Citric acid）的物质。藤黄果原产于印度，印度称此种水果树为Brindleberry，学名是Garcinia Cambogia
藤黄果提取物的简介
藤黄果提取物，也称藤黄果抽出物，是植物藤黄果的果皮抽出，精致萃取其有效份HCA(Hydroxy Citric acid；羟基柠檬酸)，含有10–30%类似柠檬酸（Citric acid）的物质。藤黄果原产于印度，印度称此种水果树为Brindleberry，学名是Garcinia Cambogia
The－SARScoronavirus－Life－Cycle
Severe Acute Respiratory Syndrome (SARS) has affected thousands of individuals, causing fever, and pneumonia with a mortality rate estimated at 10-18%
Flow－Cytometric－Analysis－of－Cell－Cycle
Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells