Aminoacidcomposition

Amino－acid－composition

There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi

Methods－for－the－Detection－of－DAminoAcid－Oxidase1

AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are de

Methods－for－the－Detection－of－DAminoAcid－Oxidase2

Results and DiscussionNavigationAbstractIntroductionMaterials and MethodsResults and DiscussionReferencesD-Amino-acid oxidase activity was detected in

氨基酸(amino－acid,－AA)含量试剂盒说明书

氨基酸(amino acid, AA)含量试剂盒说明书微量法 100管/96样 注意：正式测定之前选择 2-3 个预期差异大的样本做预测定。测定意义：动物肝脏、肾脏是氨基酸代谢的主要器官，故尿中氨基酸的变化最能反应肝、肾的生理状态。另外，氨基酸还能反应灼伤、伤寒等方面情况。植物体内氨基酸含量对研究植

Glucogenic－and－Ketogenic－Amino－Acids

A significant amount of metabolic energy can come from amino acid metabolism, particularly under conditions of starvation. The metabolism of amino aci

Acetylation－(or－Succinylation)－of－Amino－Groups－on－Proteins

Acetylation (or Succinylation) of Amino Groups on ProteinsREFERENCE: Hanock and Benz. 1986. BBA. 860:699-707.PURPOSE: Derivitization of amino groups t

Neutralizing－Arachidonic－Acid

NOTE - Use arachidonic acid from Biomol (# FA-003)1) Make up the appropriate concentration of AA in 100% ethanol.2) Add 1µl of phenol red solution.-->

Arachidonic－Acid－Labeling

1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 time with room temperature PBS.3) Resuspend

The－Citric－Acid－Cycle

The Krebs cycle, also called the citric acid cycle, is a fundamental metabolic pathway involving eight enzymes essential for energy production through

The－reactions－that－feed－amino－groups－into－the－urea－cycle

Excess amino acids in the body can be used as a source of energy, with their carbon skeleton converted to metabolic intermediates such as acetyl-CoA o

DAPI－Nucleic－Acid－Stain

实验概要The  blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it  appears to associate with AT clusters in the minor groove. Binding

Preparation－of－nucleic－acid－probes

Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s

Oxidation－of－Polyunsaturated－Fatty－Acid

Unsaturated fatty acids are those that contain one or more double bonds in their alkyl-chain. Polyunsaturated fats with two double bonds usually have

Principles－of－nucleic－acid－hybridization

Principles of nucleic acid hybridization5.2.1. Nucleic acid hybridization is a method for identifying closely related nucleic acid molecules within tw

Protocol－for－Trichloroacetic－Acid－(TCA)－Precipitation

IntroductionThe efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replica

核酸提取(The－Extraction－of－Nucleic－Acid)

【实验目的】 1．掌握核酸提取的基本原理和基本方法 2．掌握检测核酸浓度及纯度的原理及方法   【实验原理】 DNA是遗传信息的载体,是最重要的生物信息分子,是分子生物学研究的主要对象,因此DNA的提取也应是分子生物学实验技术中最重要、最基本的操作。如不能有效的完成DNA提取方面的工作，那就根本谈不

Acid－Phenol－Yeast－RNA－Prep

This is the preferred method for yeast RNA preparationuse Gloves and RNAse free solutions throughout.1. Use a YPD overnight culture to innoculate fres

RNase－inhibitors－and－RNases

Question  1.What are the differences among RNase H, RNase A, RNase B and RNase C?2.In your cDNA kits, RNase H is added in the second strand reaction t

Probiotic－Attributes－of－Lactic－Acid－Bacteria－Isolates

IntroductionPresently the consumption of probiotic food is highly popular worldwide for their health beneficial effects. Commonly used probiotic bacte

Testing－the－effects－of－retinoic－acid－on－tail－formation

耐Hydrofluoric－acid电极使用技巧

1.将耐Hydrofluoric acid电极连接到ph计的输入端，正确连接。2. 正确配制耐Hydrofluoric acid电极浸泡混合液：取37.25克KCL和1.75克混合磷酸盐粉末配制成250ml混合溶液。3.严禁用耐Hydrofluoric acid电极测量能溶解PVC以及ABS的有机溶

Biosynthesis－of－Cysteine－in－mammals

In animals, the sulfur-containing amino acid cysteine is synthesized from homocysteine, which is itself derived from metabolism of the amino acid meth

Phosphoamino－acid－analysis:Mark－Kampss－method

Phosphoamino acid analysis:Mark Kamps's method1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis

Gammaaminobutyric－Acid－Receptor－Life－Cycle

Gamma-amino butyric acid (GABA) is an inhibitory neurotransmitter that acts at synapses through binding to ligand-gated ion channels, the GABA recepto

龙眼核的化学成分

　　核含肥皂草素及脂肪。种子含三种氨基酸：2-氨基-4-甲基-5-已炔酸（2-amino-4-methyl hex-5-ynoic acid），2-氨基-4-羟甲基-5-己炔酸（2-amino-4-hydroxymethyl hex-5-ynoic acid）和2-氨基-4-羟基-6-庚炔酸（2-a

Catabolic－pathways－for－alanine,－glycine,－serine,－cysteine,－tryptophan

Amino acids in the diet have one of two fates - either they are incorporated into proteins or they are broken down for energy and metabolic intermedia

Lowry－–－Protein－Determination

Lowry – Protein Determination(From Protein Protocols on CD-ROM Humana Press, 1998 - Section 1-2 The Lowry Method for Protein Quantitation Jakob H. Wat

Phosphoamino－acid－analysis－of－nonTCA－precipitable－proteins

Phosphoamino acid analysis of non-TCA precipitable proteins1. Cut out gel slice, incubate 60 min with 1 ml 5.7 M HCl @ 110oC in 13 x 100 mm screw cap

Biosynthesis－of－Cysteine－from－serine－in－bacteria－and－plants

In animals cysteine is synthesized from homocysteine, a produce of the essential amino acid methionine. In the absence of dietary methionine, animals

欧盟评估L胱氨酸作为添加剂的安全性和有效性

　　2020年2月28日，据欧盟食品安全局（EFSA）消息，应欧盟委员会要求，欧盟动物饲料添加剂和产品（FEEDAP）研究小组就一种L-胱氨酸（L-Cystine）作为所有动物品种的饲料和饮用水的营养添加剂和感官添加剂的安全性与有效性发表科学意见。　　据了解，这种L-胱氨酸是由菠萝泛菌 （Panto