Principlesofnucleicacidhybridization
Principles of nucleic acid hybridization5.2.1. Nucleic acid hybridization is a method for identifying closely related nucleic acid molecules within two populations, a complex target population and a comparatively homogeneous probe populationDefinition of nucleic acid hybridizationNucleic acid hybridization involves mixing single strands of two sources of nucleic acids, a probe which typically consists ......阅读全文
Principles－of－nucleic－acid－hybridization
Principles of nucleic acid hybridization5.2.1. Nucleic acid hybridization is a method for identifying closely related nucleic acid molecules within tw
Preparation－of－nucleic－acid－probes
Preparation of nucleic acid probesIn standard nucleic acid hybridization assays the probe is labeled in some way. Nucleic acid probes may be made as s
DAPI－Nucleic－Acid－Stain
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
核酸提取(The－Extraction－of－Nucleic－Acid)
【实验目的】 1．掌握核酸提取的基本原理和基本方法 2．掌握检测核酸浓度及纯度的原理及方法 【实验原理】 DNA是遗传信息的载体,是最重要的生物信息分子,是分子生物学研究的主要对象,因此DNA的提取也应是分子生物学实验技术中最重要、最基本的操作。如不能有效的完成DNA提取方面的工作，那就根本谈不
Nucleic－Acid－Res：大数据库助力癌症药物的开发
近日，来自英国癌症研究所的研究人员通过研究表示，通过加入错误蛋白的3D结构，以及绘制癌症交流的图谱，就会为世界上最大的癌症药物数据库带来革命性的突破；最近更新的canSAR数据库或将为英国及全球的科学家们提供机会来设计出更加高效的癌症疗法，相关研究刊登于国际杂志Nucleic Acid Rese
Principles－of－Aseptic－Technique
INTRODUCTIONThe regulations promulgated to implement the amended Animal Welfare Act require that all survival surgery be performed using aseptic proce
CDNA文库
CDNA文库（主要内容如下）· Construction of cDNA Library· Construction of Genome DNA Library· Library Screening OthersConstruction of cD
Nuclear－RunOn－Transcription－Assays
Nuclear “run-on” (or “run-off”) transcription assays have been used to obtain quantitative information about the relative rates of transcription o
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Principles－of－Aseptic－Technique——3
PREPARATION OF THE ANIMALThe animals should be prepared in a n area separate from where surgery will be performed. Preparation is facilitated by first
Principles－of－Aseptic－Technique——2
PersonnelAseptic technique requires careful attention to a series of steps which begins with patient and instrument preparation and ends at final woun
Principles－of－Aseptic－Technique——4
P REPARATION OF THE SURGEONIn a laboratory setting, the extent of surgeon preparation will depend on the facilities and the need for strict attention
Colony－Hybridization
ProcedurePrepare serial 10-fold dilutions of transformed bacteria in LB and spread 100 µL onto LB/Amp plates, as described in the Electroporation prot
In－situ－hybridization
Note: Although it is possible to perform in situ hybridization on bleached embryos, it appears to reduced the strength of the signal. For best results
COLONY－HYBRIDIZATION
COLONY HYBRIDIZATION1) CUT 4 PIECES OF 3MM WHATMAN PAPER AND PLACE EACH ONE IN A SEPARATE CONTAINER(A CAFETERIA TRAY WILL PROBABLY WORK PERFECTLY).2)
Microarray－Hybridization－Protocol
Introduction:One microarray set consists of 7 nylon membranes with 2.5 x 7.5 cm dimension. 2304 genes were spotted onto nylon membranes (Schleicher an
How－to－use－Basic－Local－Alignment－Search－Tool－(BLAST)
DescriptionThe BLAST algorithm was developed as a way to perform DNA and protein sequence similarity searches by an algorithm that is faster than FAST
Silver－Acetate－Autometallography－(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for l
Arachidonic－Acid－Labeling
1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 time with room temperature PBS.3) Resuspend
Amino－acid－composition
There has been a recent revival of interest in the use of AA composition for the identification of proteins from 2-D gels. This technique uses the idi
The－Citric－Acid－Cycle
The Krebs cycle, also called the citric acid cycle, is a fundamental metabolic pathway involving eight enzymes essential for energy production through
Neutralizing－Arachidonic－Acid
NOTE - Use arachidonic acid from Biomol (# FA-003)1) Make up the appropriate concentration of AA in 100% ethanol.2) Add 1µl of phenol red solution.-->
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens
INTRODUCTIONFlow cytometry is frequently used to assess nucleic acid content in individual cells. Based on DNA content alone, however, cells in the qu
ORNL－MICROARRAY－HYBRIDIZATION－PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5:A. MATERIALSRNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat #
细胞遗传学——原位杂交(ISH)
In Situ Hybridization· In Situ Hybridization (jsmith1@po-box.mcgill.ca)In situ hybridization, as the name suggests, is a method of localizing,
nucleic－acids－research影响因子
《NUCLEIC ACIDS RESEARCH》是一个期刊，最新的影响因子为11.561。 NUCLEIC ACIDS RESEARCH 出版国家：England 出版商：Oxford University Press 出版周期：Semimonthly 语言：English 研究领域
Oxidation－of－Polyunsaturated－Fatty－Acid
Unsaturated fatty acids are those that contain one or more double bonds in their alkyl-chain. Polyunsaturated fats with two double bonds usually have
Screening－BAC－filters－with－nonradioactive－probes
(Protocol for a single high-density BAC colony filter (HDR filter) of 22 cmx22 cm) to be screened using Amersham''s ECL hybridization kit; thi
Making－RNA－probes－for－in－situ－hybridization
Make DNA templates via PCRDay 1It's preferable to start with constructs that contain RNA polymerase start sites (T3, T7, or SP6) which allow use o
Fluorescence－In－Situ－Hybridization－using－TSA™
实验概要This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (TSA™), and