BiosynthesisofTryptophaninBacteriaandPlants

The aromatic amino acid tryptophan is an essential nutrient, meaning that humans and animals do not themselves have the biosynthetic machinery to synthesize tryptophan but rely on dietary intake from bacteria and plants that do produce it. Tryptophan serves not only as a building block for protein synthesis but a precursor for synthesis of serotonin (see Neurotransmitter biosynthesis pathway). The tryptophan biosynth......阅读全文

Biosynthesis-of-Tryptophan-in-Bacteria-and-Plants

The aromatic amino acid tryptophan is an essential nutrient, meaning that humans and animals do not themselves have the biosynthetic machinery to synt

Biosynthesis-of-Chorismate-in-Bacteria-and-Plants

The biosynthesis of all three aromatic amino acids (tryptophan, tyrosine and phenylalanine) begins with the metabolic intermediate chorismate. The bio

Biosynthesis-of-Cysteine-from-serine-in-bacteria-and-plants

In animals cysteine is synthesized from homocysteine, a produce of the essential amino acid methionine. In the absence of dietary methionine, animals

Biosynthesis-of-Proline-in-Bacteria

Among the twenty amino acids encoded by the genetic code, proline is unique in having a cyclic structure with its side chain connected to the amino gr

Biosynthesis-of-isoleucine

Isoleucine is an essential amino acid, only synthesized in plants and bacteria, and required in the diet by animals. In proteins, the hydrophobic isol

Bacteria-Growth-and-Culture-Bacteria-Growth-and-Culture

Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w

Biosynthesis-of-neurotransmitters

Neurotransmitters are signaling molecules used by neurons to communicate across chemical synapses. The receptors for neurotransmitters include G-prote

Biosynthesis-and-Analysis-of-Bilins

The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first o

Phosphatidylcholine-Biosynthesis-Pathway

The main components of biological membranes are phosphoglyceride lipids composed of a glycerol unit esterified to two fatty acids and a polar alcohol

Biosynthesis-of-Glycine-and-Serine

Intermediates in energy production pathways such as glycolysis and the Kreb's cycle are commonly the starting point for the biosynthesis of amino

Biosynthesis-of-Cysteine-in-mammals

In animals, the sulfur-containing amino acid cysteine is synthesized from homocysteine, which is itself derived from metabolism of the amino acid meth

Biosynthesis-of-spermidine-and-spermine

The polyamines spermidine and spermine are expressed in a variety of tissues and are involved in the regulation of apoptosis, cellular proliferation,

Testing-for-Bacteria-and-Fungi

AimIn cases of gross contamination the naked eye may identify the presence of bacteria and fungi. However, it is necessary to detect low-level infecti

Bacteria-Culture-Protocol

Bacteria Culture ProtocolBy 徐晓政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce

Catabolic-pathways-for-alanine,-glycine,-serine,-cysteine,-tryptophan

Amino acids in the diet have one of two fates - either they are incorporated into proteins or they are broken down for energy and metabolic intermedia

Streaking-Bacteria-for-Single-Colonies

1. Initial inoculum:- From solid media, touch an isolated colonies with a sterile applicator or toothpick.- From liquid media, touch the culture with

Transformation-Protocol-for-Arabidopsis

Transformation Protocol for Arabidopsis – Abbreviated Germinate seed in pots ↓ 4 weeks Streak bacteria onto YM/MinA ↓ 2-3 days 28°C Spray/dip ba

专化性的麦角甾醇合成抑制剂

α-Bisabolol from Chamomile – A specific ergosterol biosynthesis inhibitor? SummaryThere is a strong need for new antifungal agents with a differen

Isolation-of-genomic-DNA-from-bacteria

Note: This procedure does not work well with Gram + cocci. Transfer 1.5 mL overnight culture to a 1.5 mL microfuge tube, centrifuge for 30 sec, de

Agar-Plates-for-Selection-of-Clones-in-Bacteria

Cloning of PCR products Stocks: LB Agar: Luria Broth after Lennox: per Liter Tryptone 10 g Yeast Extract 5 g Sodium Chlori

Bacteria,-cont.-Growth-and-Reproduction

Flagella and motility monotrichous flagella - the bacterial cell has a single flagella peritrichous flagella - the bacterial cell has several f

In-Vitro-Conservation-and-Cryopreservation-of-Ornamental-Plants

Today, the conservation of ornamental germplasm can take advantage of innovative techniques which allow preservation in vitro (slow growth storage

Phospholipid-Biosynthesis-in-E.-Coli-Pathway

The biosynthesis of membrane phospholipids occurs through distinct pathways in mammals and bacteria. In the mammalian pathway for the synthesis of pho