BiosynthesisofTryptophaninBacteriaandPlants
The aromatic amino acid tryptophan is an essential nutrient, meaning that humans and animals do not themselves have the biosynthetic machinery to synthesize tryptophan but rely on dietary intake from bacteria and plants that do produce it. Tryptophan serves not only as a building block for protein synthesis but a precursor for synthesis of serotonin (see Neurotransmitter biosynthesis pathway). The tryptophan biosynth......阅读全文
Biosynthesis-of-Tryptophan-in-Bacteria-and-Plants
The aromatic amino acid tryptophan is an essential nutrient, meaning that humans and animals do not themselves have the biosynthetic machinery to synt
Biosynthesis-of-Chorismate-in-Bacteria-and-Plants
The biosynthesis of all three aromatic amino acids (tryptophan, tyrosine and phenylalanine) begins with the metabolic intermediate chorismate. The bio
Biosynthesis-of-Cysteine-from-serine-in-bacteria-and-plants
In animals cysteine is synthesized from homocysteine, a produce of the essential amino acid methionine. In the absence of dietary methionine, animals
Biosynthesis-of-Proline-in-Bacteria
Among the twenty amino acids encoded by the genetic code, proline is unique in having a cyclic structure with its side chain connected to the amino gr
Biosynthesis-of-isoleucine
Isoleucine is an essential amino acid, only synthesized in plants and bacteria, and required in the diet by animals. In proteins, the hydrophobic isol
Bacteria-Growth-and-Culture-Bacteria-Growth-and-Culture
Flagella and motilitymonotrichous flagella - the bacterial cell has a single flagellaperitrichous flagella - the bacterial cell has several flagella w
Biosynthesis-of-neurotransmitters
Neurotransmitters are signaling molecules used by neurons to communicate across chemical synapses. The receptors for neurotransmitters include G-prote
Biosynthesis-and-Analysis-of-Bilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first o
Biosynthesis-of-threonine-and-methionine
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Phosphatidylcholine-Biosynthesis-Pathway
The main components of biological membranes are phosphoglyceride lipids composed of a glycerol unit esterified to two fatty acids and a polar alcohol
Biosynthesis-of-Glycine-and-Serine
Intermediates in energy production pathways such as glycolysis and the Kreb's cycle are commonly the starting point for the biosynthesis of amino
Biosynthesis-of-Cysteine-in-mammals
In animals, the sulfur-containing amino acid cysteine is synthesized from homocysteine, which is itself derived from metabolism of the amino acid meth
Biosynthesis-of-spermidine-and-spermine
The polyamines spermidine and spermine are expressed in a variety of tissues and are involved in the regulation of apoptosis, cellular proliferation,
Testing-for-Bacteria-and-Fungi
AimIn cases of gross contamination the naked eye may identify the presence of bacteria and fungi. However, it is necessary to detect low-level infecti
Bacteria-Culture-Protocol
Bacteria Culture ProtocolBy 徐晓政1、TBS Medium Preparation:Prepare 1L of TBS medium contains:Tryptone 12gYeast extract 24gNaCl 5gSodium Succinate 5gGlyce
Catabolic-pathways-for-alanine,-glycine,-serine,-cysteine,-tryptophan
Amino acids in the diet have one of two fates - either they are incorporated into proteins or they are broken down for energy and metabolic intermedia
Streaking-Bacteria-for-Single-Colonies
1. Initial inoculum:- From solid media, touch an isolated colonies with a sterile applicator or toothpick.- From liquid media, touch the culture with
Transformation-Protocol-for-Arabidopsis
Transformation Protocol for Arabidopsis – Abbreviated Germinate seed in pots ↓ 4 weeks Streak bacteria onto YM/MinA ↓ 2-3 days 28°C Spray/dip ba
专化性的麦角甾醇合成抑制剂
α-Bisabolol from Chamomile – A specific ergosterol biosynthesis inhibitor? SummaryThere is a strong need for new antifungal agents with a differen
Isolation-of-genomic-DNA-from-bacteria
Note: This procedure does not work well with Gram + cocci. Transfer 1.5 mL overnight culture to a 1.5 mL microfuge tube, centrifuge for 30 sec, de
Agar-Plates-for-Selection-of-Clones-in-Bacteria
Cloning of PCR products Stocks: LB Agar: Luria Broth after Lennox: per Liter Tryptone 10 g Yeast Extract 5 g Sodium Chlori
Bacteria,-cont.-Growth-and-Reproduction
Flagella and motility monotrichous flagella - the bacterial cell has a single flagella peritrichous flagella - the bacterial cell has several f
In-Vitro-Conservation-and-Cryopreservation-of-Ornamental-Plants
Today, the conservation of ornamental germplasm can take advantage of innovative techniques which allow preservation in vitro (slow growth storage
Phospholipid-Biosynthesis-in-E.-Coli-Pathway
The biosynthesis of membrane phospholipids occurs through distinct pathways in mammals and bacteria. In the mammalian pathway for the synthesis of pho