BiosynthesisandAnalysisofBilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first of these compounds to be characterized were isolated from animal bile. These bilins, biliverdin (BV) and bilirubin (BR), are the sequential products of heme degradation (their green and yellow pigmentation can be detected during the discoloration of a bruise), with ......阅读全文
Biosynthesis－and－Analysis－of－Bilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first of
Biosynthesis－of－neurotransmitters
Neurotransmitters are signaling molecules used by neurons to communicate across chemical synapses. The receptors for neurotransmitters include G-prote
Biosynthesis－of－isoleucine
Isoleucine is an essential amino acid, only synthesized in plants and bacteria, and required in the diet by animals. In proteins, the hydrophobic isol
Biosynthesis－of－Glycine－and－Serine
Intermediates in energy production pathways such as glycolysis and the Kreb's cycle are commonly the starting point for the biosynthesis of amino
Biosynthesis－of－threonine－and－methionine
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Biosynthesis－of－spermidine－and－spermine
The polyamines spermidine and spermine are expressed in a variety of tissues and are involved in the regulation of apoptosis, cellular proliferation,
Biosynthesis－of－Cysteine－in－mammals
In animals, the sulfur-containing amino acid cysteine is synthesized from homocysteine, which is itself derived from metabolism of the amino acid meth
Biosynthesis－of－Proline－in－Bacteria
Among the twenty amino acids encoded by the genetic code, proline is unique in having a cyclic structure with its side chain connected to the amino gr
Phosphatidylcholine－Biosynthesis－Pathway
The main components of biological membranes are phosphoglyceride lipids composed of a glycerol unit esterified to two fatty acids and a polar alcohol
Biosynthesis－of－Chorismate－in－Bacteria－and－Plants
The biosynthesis of all three aromatic amino acids (tryptophan, tyrosine and phenylalanine) begins with the metabolic intermediate chorismate. The bio
Biosynthesis－of－Tryptophan－in－Bacteria－and－Plants
The aromatic amino acid tryptophan is an essential nutrient, meaning that humans and animals do not themselves have the biosynthetic machinery to synt
Glycosphingolipid－analysis
1) Incubate cells with 1 µCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media containing
Lipid－analysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer
Analysis－of－Heme－and－Hemoproteins
Heme is perhaps the most ubiquitous cofactor found in nature and the most functionally diverse. Hemoproteins are involved in cell respiration (cyt
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Lineage－Analysis－of－Blood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow
Analysis－of－Oligosaccharide－Ligands
Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity ChromatographyAnalysis of Oligosaccharide Ligands by High Performance Liquid A
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Analysis－of－murine－BM
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in huma
Biosynthesis－of－Cysteine－from－serine－in－bacteria－and－plants
In animals cysteine is synthesized from homocysteine, a produce of the essential amino acid methionine. In the absence of dietary methionine, animals
Phospholipid－Biosynthesis－in－E.－Coli－Pathway
The biosynthesis of membrane phospholipids occurs through distinct pathways in mammals and bacteria. In the mammalian pathway for the synthesis of pho
Analysis－and－Reconstitution－of－Phycobiliproteins:－Methods－for－the－...
Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment ReactionsPhycobiliproteins are a homologous fam
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
FACS－Analysis－of－ES－Cells
Isolate cells and dissociate to single cell suspension (can use Gibco Cell Dissociation Buffer, Accutase or Trypsin)Wash with 10% FBS/DMEM:F12For surf