PAM:PredictionAnalysisforMicroarrays
PAM: Prediction Analysis for MicroarraysClass Prediction and Survival Analysis for Genomic Expression Data Mining Features:Performs sample classification from gene expression data,via "nearest shrunken centroid method'' of Tibshirani, Hastie, Narasimhan and Chu (2002): "Diagnosis of multiple cancer types by shrunken centroids of gene expression" (PNAS website). PNAS 2002 99:6567-......阅读全文
PAM:－Prediction－Analysis－for－Microarrays
PAM: Prediction Analysis for MicroarraysClass Prediction and Survival Analysis for Genomic Expression Data Mining Features:Performs sample classificat
Peptide－map－prediction
Peptide map prediction In identifying peptides from proteins with a known sequence, it is often useful to be able to predict how a peptide will migr
Peptide－map－prediction
In identifying peptides from proteins with a known sequence, it is often useful to be able to predict how a peptide will migrate during electrophoresi
Microarrays－to－Characterize－the－MolecularGenetic－Basis－of－Disease
article by Dr. RL McInnes, Agilent Technologies, AustraliaGenomic instability underlies cancer and Copy Number Aberrations (CNA s) are known to underp
Glycosphingolipid－analysis
1) Incubate cells with 1 µCi/ml of 3H-galactose for 72 hours.---> If treatment is for an extended period of time: treat in serum free media containing
Lipid－analysis
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer
什么是PAM？
聚丙烯酰胺（PAM）为水溶性高分子聚合物，不溶于大多数有机溶剂，具有良好的絮凝性，可以降低液体之间的磨擦阻力，按离子特性分可分为非离子、阴离子、阳离子和两性型四种类型。
Biosynthesis－and－Analysis－of－Bilins
The term bilin is a collective one to describe a broad group of open chain tetrapyrroles and derives from the name “bile pigments” as the first o
Analysis－of－Heme－and－Hemoproteins
Heme is perhaps the most ubiquitous cofactor found in nature and the most functionally diverse. Hemoproteins are involved in cell respiration (cy
Analysis－of－Oligosaccharide－Ligands
Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography Analysis of Oligosaccharide Ligands by High Performance Liqui
Analysis－of－murine－BM
Histologic analysis of murine BM is a necessary complement to flow cytometric or in vitro analysis. Techniques to do this are well established in hu
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Lineage－Analysis－of－Blood
Materials:Capillary tubes1.5 mL Eppendorf microfuge tubes15 mL conical centrifuge tubes96-well V-bottom plates (Corning Costar 3894, from Fisher)Flow
PAM的使用特性
使用特性1、絮凝性：PAM能使悬浮物质通过电中和，架桥吸附作用，起絮凝作用。2、粘合性：能通过机械的、物理的、化学的作用，起粘合作用。3、降阻性：PAM能有效地降低流体的摩擦阻力，水中加入微量PAM就能降阻50—80%。4、增稠性：PAM在中性和酸条件下均有增稠作用，当PH值在10以上PAM易水解。
蛋白质结构预测(protein－structure－prediction)
一种生物体的基因组规定了所有构成该生物体的蛋白质，基因规定了组成蛋白质的氨基酸序列。虽然蛋白质由氨基酸的线性序列组成，但是，它们只有折叠成特定的空间构象才能具有相应的活性和相应的生物学功能。了解蛋白质的空间结构不仅有利于认识蛋白质的功能，也有利于认识蛋白质是如何执行其功能的。确定蛋白质的结构对于生物
Genomic－Southern－Blot－Analysis
This chapter describes a detailed protocol for genomic Southern blot analysis which can be used to detect transgene or endogenous gene sequences
Analysis－and－Reconstitution－of－Phycobiliproteins:－Methods－for－the－...
Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment Reactions Phycobiliproteins are a homologous
Microtubule－Spindowns－for－Visual－Analysis
Microtubule spindowns for visual analysis can be performed on single microtubules or microtubules nucleated from axonemes/centrosomes. Although live
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Pr
Molecular－Analysis－and－Results－－DNA
Theory of CGHComparative genomic hybridization (CGH) is a fairly new molecular cytogenetic technique that allows detection of DNA sequence copy number
FACS－Analysis－of－ES－Cells
Isolate cells and dissociate to single cell suspension (can use Gibco Cell Dissociation Buffer, Accutase or Trypsin)Wash with 10% FBS/DMEM:F12For surf
Simultaneous－analysis－of－DNA－content
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. William Telford. Louis E. King and Pamela
PAM沉淀的技术流程
沉淀是发生化学反应时生成了不溶于反应物所在溶液的物质。从字意上理解就是在重力作用下沉淀去除。污水中的悬浮物质，可以这是一种物理过程，简便易行，效果良好，是污水处理的重要技术之一。根据悬浮物质的性质、浓度及絮聚丙烯酰胺凝性能，沉淀可以分为：自然沉淀，絮凝沉淀，区域沉淀。域沉淀的悬浮颗泣浓度较高(500
PAM沉淀的技术流程
沉淀是发生化学反应时生成了不溶于反应物所在溶液的物质。从字意上理解就是在重力作用下沉淀去除。污水中的悬浮物质，可以这是一种物理过程，简便易行，效果良好，是污水处理的重要技术之一。 根据悬浮物质的性质、浓度及絮聚丙烯酰胺凝性能，沉淀可以分为：自然沉淀，絮凝沉淀，区域沉淀。域沉淀的悬浮颗泣浓度较高
PAM沉淀的技术流程
沉淀是发生化学反应时生成了不溶于反应物所在溶液的物质。从字意上理解就是在重力作用下沉淀去除。污水中的悬浮物质,可以这是一种物理过程,简便易行,效果良好,是污水处理的重要技术之一。 根据悬浮物质的性质、浓度及絮聚丙烯酰胺凝性能,沉淀可以分为：自然沉淀,絮凝沉淀,区域沉淀。域沉淀的悬浮颗泣浓度较高
PAM沉淀的技术流程
PAM沉淀的技术流程沉淀是发生化学反应时生成了不溶于反应物所在溶液的物质。从字意上理解就是在重力作用下沉淀去除。污水中的悬浮物质，可以这是一种物理过程，简便易行，效果良好，是污水处理的重要技术之一。根据悬浮物质的性质、浓度及絮聚丙烯酰胺凝性能，沉淀可以分为：自然沉淀，絮凝沉淀，区域沉淀。域沉淀的悬浮
PAM沉淀的技术流程
沉淀是发生化学反应时生成了不溶于反应物所在溶液的物质。从字意上理解就是在重力作用下沉淀去除。污水中的悬浮物质，可以这是一种物理过程，简便易行，效果良好，是污水处理的重要技术之一。根据悬浮物质的性质、浓度及絮聚丙烯酰胺凝性能，沉淀可以分为：自然沉淀，絮凝沉淀，区域沉淀。域沉淀的悬浮颗泣浓度较高(500
PAM沉淀的技术流程
沉淀是发生化学反应时生成了不溶于反应物所在溶液的物质。从字意上理解就是在重力作用下沉淀去除。污水中的悬浮物质，可以这是一种物理过程，简便易行，效果良好，是污水处理的重要技术之一。根据悬浮物质的性质、浓度及絮聚丙烯酰胺凝性能，沉淀可以分为：自然沉淀，絮凝沉淀，区域沉淀。域沉淀的悬浮颗泣浓度较高(500
TISSUE－FIBROBLAST－CULTURES－FOR－CHROMOSOME－ANALYSIS
I. Purpose: A: Skin tissue may be used for chromosome analysis in special cases when the results from peripheral blood are inconclusive, e.g. suspec