LiveCellImagingofYeast
Live Cell Imaging of YeastDaniel R. Rines, Dominik Thomann, Jonas F. Dorn, Paul Goodwin and Peter K. SorgerINTRODUCTIONThe development of cloning vectors for green fluorescent protein (GFP) and the simplicity of yeast reverse genetics allow straightforward labeling of yeast proteins in living cells. Budding and fission yeast are therefore attractive organisms in which to study dynamic cellular processes such as growt......阅读全文
微生物所在微管骨架动态组织机制研究中获进展
微管(Microtubules, MTs)是真核生物细胞骨架的重要组分,在各种细胞过程中都发挥重要作用,如细胞形态决定、细胞分裂、细胞运动、胞内物质运输和信号传导等。微管骨架具有高度的动态特性,其排列方式不断进行活跃的重组,以响应发育和外界环境(包括生物和非生物刺激)信号。动物细胞中的微管组织中
蛋白质提取和纯化
蛋白质提取和纯化(主要内容如下)Protein Extraction Protein PurificationProtein PrecipitationColumn PreparatioinQ & A Posted in the Method ForumProtein ExtractionWhole
细胞核提取
HeLa Cell Nuclei Preparation (John Garland)Prepare nuclear extract from HeLa cell · Extract Preparation (Brent Graveley)Preparation of nuclea
Cell-to-Cell-Adhesion-Signaling
Interactions between cells responsible for cell to cell adhesion also can communicate signals into the cellular interior, often involving interactions
Vybrant®-DyeCycle™-Ruby-stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
Layered-plates
General InformationThe cells being plated are sandwiched between layers of cell-free agar. The submerged cells grow into smaller colonies and many mor
细胞培养常规操作
常规操作(主要内容如下)· Aseptic Technique· Culture Vessels· Cell Counting· Primary Culture· Maintenance of Cell Line ·
ASENSITIVE-METHOD-FOR-DETECTION-OF-APOPTOSIS-BY-SINGLE-LASER-FLOW-CYTOMETRY
MATERIALS:1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide)2. 7-Amino-actinomycin D (7-
AbC™-AntiMouse-Bead-Kit
实验概要The AbC™ Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when u
Handling-Fresh-Hepatocytes-in-Suspension
实验概要Important—Please read!GIBCO® Fresh Hepatocytes are shipped in cold preservation medium designed to keep cells viable at 4°C. This shipping mediu
Derivation-and-Culture-of-Cortical-Astrocytes
实验概要Astrocytes are the most numerous cell type in the central nervous system (CNS). They play critical roles in adult CNS homeostasis, provide bioch
Fluidigm公司微液流芯片在单细胞研究中的应用(二)
It was also worth it for Tannishtha Reya, who studies stem cell fate and cancer at Duke University Medical Center in Durham, North Carolina. "Im
Cell-Thawing/Cell-Freezing-Protocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media
Cell-Thawing/Cell-Freezing-Protocol
Freezing Cells:Cells should be growing well or known to be in log phaseCount, collect and pellet cells in a 15mL test tubeResuspend in freezing media
线粒体荧光探针大全:TMRM,Mitotracker,JC1(2)
MitoTracker Green FM ProbeMitochondria in cells stained with nanomolar concentrations of our patented MitoTracker Green FM dye (M7514) exhibit bright
AbC™-AntiRat/Hamster-Bead-Kit
实验概要The AbC™ anti-Rat/Hamster Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation
AbC™-AntiRat/Hamster-Bead-Kit
实验概要The AbC™ anti-Rat/Hamster Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation
线粒体荧光探针大全:TMRM,Mitotracker,JC1(1)
线粒体荧光探针信息大全 (Probes for Mitochondria)包括各种常用探针,如JC-1,JC-9,TMRM,TMRE等Mitochondria are found in eukaryotic cells, where they make up as much as 10% of th
酵母转化的几种方法
Modified Yeast Transformation Inoculate cells from an overnight culture into 50 ml YEPD and incubate at 30°C with shaking. Typically, add 0.1 to 0.2 m
Flow-Cell-Assays-with-Microtubules:-Motility/Dynamics-in-Fluorescence
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
酵母染色体沉淀分析方法
ABSTRACTThis protocol describes a method for the detection of proteins bound to specific regions of chromatin in yeast. There are many variations of t
Optimized-Method-for-the-Preparation-of-Rodent-Testicular-Cells2
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensio
光片显微镜的前世今生
光片荧光显微镜(Light Sheet Fluorescence Microscopy, LSFM)的概念产生于1903年,但此后很长时间并无太多发展。上世纪九十年代,华盛顿大学的Francis Spelman实验室为了对小鼠毛细胞的结构和耳蜗的其他特性进行定量测量,发展了一系列实验方法
cdc25-and-chk1-Regulatory-Pathway-in-response-to-DNA-damage
Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells i
DAPI染DNA的原理及使用方法
DAPI 即4',6-二脒基-2-苯基吲哚(4',6-diamidino-2-phenylindole),分子式为C16 H15 N5 ·2C3 H6 O3 ,分子量457.48。DAPI 是一种能够与DNA强力结合的荧光染料。它结合到双链DNA小沟的AT碱基对处,一个DAPI分子可
DAPI染DNA的原理及使用方法
DAPI 即4',6-二脒基-2-苯基吲哚(4',6-diamidino-2-phenylindole),分子式为C16 H15 N5 ·2C3 H6 O3 ,分子量457.48。DAPI 是一种能够与DNA强力结合的荧光染料。它结合到双链DNA小沟的AT碱基对处,一个DAPI分子
High-Efficiency-Transformation
Day 0Make sure you have the necessary solutions (instructions for how to make them can be found here):Single-stranded carrier DNAPEG 3350 50% w/vol1.0
超声波成像设备研发商MAUI-Imaging获得1400万美元D轮融资
Acertara声学实验室引领FDA认证的成像技术,揭示传统超声设备无法显示的解剖结构 亚利桑那州图森市,2025年7月29日 —— MAUI Imaging公司是拥有专利技术的发明者,该技术能够可视化其他超声技术无法显示的解剖结构。该公司今日宣布在D轮融资中筹集了1400万美元,包括股权融资
Cell-Stem-Cell-|-连续取得进展!
下丘脑含有惊人的异质性神经元,可调节内分泌,自主神经和行为功能。然而,其分子发育轨迹和神经元多样性的起源仍不清楚。2021年4月21日,中国科学院遗传与发育生物学研究所吴青峰团队在Cell Stem 在线发表题为“Cascade diversification directs generati
AbC™-AntiMouse-Bead-Kit
实验概要The AbC™ Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when u