PreparationandStainingofParaffinSections
I. Fixation and Processing of Tissue for Paraffin SectionsA. Fixation of Tissues in 10% Neutral Buffered FormalinSacrifice animal by prescribed and approved euthanasia techniques. Tissues to be fixed and processed should be cut to a size no larger than 3mm thick. Let tissues fix in 10% formalin at room temperature for 8 hours but not to exceed 24 hours. For small rodent tissue, it is recommended to fix tissues for 4-......阅读全文
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells3
Moreover, when the method was applied to the analysis of the cellular composition of immature testes, the results were also in agreement with previous
Method:－Reactivating－Cell－Lines－and－Cell－Growth－for－DNA－Preparation
Purpose:Cell lines are reactivated and grown to a count of 1 x 108 cells. The cells are pelleted and stored frozen at -80 degrees C prior to DNA extra
Method:－Preparation－of－Lymphoblastoid－Cell－Lines－for－Long－Term－Storage
Method: Preparation of Lymphoblastoid Cell Lines for Long Term StorageMay 30, 1990Rosalie VeilePurpose:To store cell lines in a form that will insure
DNA转染
DNA转染· Transfection of Mammalian Cells Using Lipofectamine (LTI)· Guide to Eukaryotic Transfections with Cationic Lipid Reagents (PDF)
Western－杂交
Western 杂交（主要内容如下）Preparing of Protein LysatesWestern BlottingFar Western BlottingSemi Dry BlottingStripping MembranesTrouble Shooting and OthersPrepa
Staining－Methods－for－cell－death－Z.－Xia－10/2/95
The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead
Application－Note:－Qdot®－Nanocrystal－Conjugates－in－Flow－Cytometry
实验概要Researchers today are trying to maximize the information that they get out of flow cytometry experiments by looking at more parameters in a sing
无创血压计应用论文：动物用血压计（二）
Measurements of Blood Pressure and Heart Rate-Systolic blood pressure and heart rate were measured using a noninvasive computerized tail-cuff syst
Heterogeneity－of－SingleCell－Gene－Expression－Across－Phenotypically（一）
Introduction Multi-cellular populations are fundamentally driven by the collective properties of individual cells. However, our understanding of ge
Immunocytochemistry...
实验概要The method provides a guideline procedure for staining of cell cultures using immunofluoresence.实验步骤1. General procedure 1) Coat coverslips wit
Culture－of－Peripheral－Blood－Lymphocytes－for－Chromosome－Analysis
实验概要Provide information about chromosomal abnormalities.实验原理The blood cell karyotyping method was developed to provide information about chromosomal
Look－Ma,－No－Archenteron!－Sulfates－role－in－sea－urchin－early－development
ObjectiveTo observe the role sulfate plays in sea urchin gastrulation, and to replicate the findings of Karp and Solursh, that sea urchin embryos fail
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
Preparation－of－cytoplasmic－extracts－forthe－application－in－acellfree－system
DescriptionCells are grown to 80% confluency, then harvested, washed and disrupted in KPM buffer by freezing-thawing cycles with liquid nitrogen essen
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
3－Color－Staining:－AlphaCatenin－in－HeLa－Human－Cervical－Cancer－Cells
实验概要Alpha-catenin in HeLa human cervical cancer cells was labeled using mouse anti-α-catenin and visualized with Alexa Fluor® 488 goat anti-mouse Ig
低背景的蛋白质银染(silver－staining)方法
我们做蛋白质电泳的人都知道，银染色很灵敏，有很强的说服力，但通常胶背景较深，不易扫描。在这里介绍一下低背景的蛋白质银染方法。Step Reagent Time/minFix 50%EtOH, 12%HAC, 0.1%HCHO, 38%H2O 60Rinse 50% EtOH 5 min, 3 tim
ELECTRON－MICROSCOPY
E.M. PROCESSING SCHEDULE - EPOXY RESINFix tissue in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer at 4oC, for a minimum of 4 hours. Tissue shou
Microscopes－in－Cell－Biology
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
The－Effects－of－NiCl2on－Spicule－Formation
The Effects of NiCl2on Spicule FormationJessica Ann Billet, Franklin and Marshall, Class of 2000Background and ObjectiveSea urchins exhibit radial hol
Immunostaining－Thin－Layer－Chromatograms－Of－Glycolipids
Immunostaining Thin Layer Chromatograms Of GlycolipidsJohn L. Magnani~GlycoTech Corporation, Rockville, Maryland 20850Immunostaining thin layer chroma
ImmunohistochemistyFluorescence－Protocol2
Suitable for use on single-cell suspensions from peripheral blood, lymphoid tissue or cultured cell-lines.Sample Preparation and FixationHarvest cells
Placental－trophoblast－and－chorionic－cell－cultures
1. Placental trophoblast and chorionic trophoblast cells were prepared using a modification of the method. 2. Term human placentae and chorion tissue
Autoradiography
MaterialsH-Thymidine, specific activity of 2.0curie/millimoleOnion sets, jars and toothpicksAlcohol-acetic acid fixativeMaterials for feulgen reaction
Vacuum/Spin－Protocol－for－Tissue－DNA－Extraction
实验概要The E.Z.N.A.® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to 3
RNA－analysis－on－nondenaturing－agarose－gel－electrophoresis
实验概要RNA analysis on non-denaturing agarose gel electrophoresis实验步骤1. The following gel electrophoresis conditions are recommended:- use 1X TAE buffer
RNA－analysis－on－nondenaturing－agarose－gel－electrophoresis
1. The following gel electrophoresis conditions are recommended:- use 1X TAE buffer instead of 1X TBE- use agarose gel in the concentration of 1.1%-1.
PCR的下游应用
· Agarose Gel Electrophoresis of PCR Products (Robert H. Cruickshank)· Agarose Gel Electrophoresis of PCR Products (Immunology Resourc
蛋白质电泳技术2
Decrease toxicity of destain even more!The destain procedure can be made even less toxic by replacing the destaining solution completely with MilliQ w
Embryonic－limb－bud－culture－in－media
Early in embryonic development, the region of the chick embryo which is determined to form a limb first differentiates from the rest of the embryo1. T