Protocolsforthepreparationoftumourcellsfors.c.injectionsinmice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile plastic pipettes and flasks are used. Cultures are grown in a BB6220 incubator (Heraeus) at 37 °C and 6% CO2.The tumour models used in our laboratory are obtained by subcutaneous (s.c.) injection of the following cell lines in nude mice:C51 mouse colon adenocarcinomaFE-......阅读全文
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
Protocols－for－the－preparation－of－tumour－cells－for－s.c.－injections－in－mice
For the establishment of solid tumour in nude mice, tumour cells are treated in a sterile environment under a cell culturing hood. Disposable sterile
Protocols－for－LCM－preparation－and－analysis
Protocols for LCM preparation and analysis I. Preparation, LCM and RNA/DNA extraction of Frozen Tissue SectionsA. EmbeddingB. CuttingC. StainingII. Pr
活体成像皮下成瘤实验操作方法
Materials:MDA-MB-231-Luc and HCT116-Luc cellsMice: 2 subcutaneous HCT116-Luc tumor-bearing female BALB/c nude mice;4 naïve female BALB/c nude mice;Art
活体成像小鼠皮下瘤模型实验步骤
Luciferin Preparation1. Prepare a stock solution of luciferin at 15mg/ml in DPBS. Filter sterilize through a 0.2 um filter.2. Prepare enough to
Harvesting－Hematopoietic－Cells－from－Mice
Materials4 mice from each genotype4 Ly5 miceBuckets with wet ice 3xBucket with dry ice 1xDewar flask with liquid nitrogen100 mL beakers with 95% ethan
Preparation－of－Lactobacillus－Competent－Cells
OverviewInstructions on how to prepare Lactobacillus plantarum competent cells before electrotransformation.MaterialsMRS mediaCulture of L. plantarum
Preparation－Of－Peripheral－Blood－Cells－For－Chromosome－Analysis
实验概要Lymphocytes are differentiated cells which normally do not undergo subsequent cell divisions. By culturing lymphocytes in the presence of a mito
BrdU－Labeling－Protocol
实验概要The thymidine analog, 5-bromo-2-deoxyuridine (BrdU),is a common reagent used for cell proliferation assays and for the detection of apoptotic
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Adherent－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, et
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
96Well－Sample－Preparation－for－Adherent－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with adherent cells (HeLa, MCF-7, BALB/c 3T3, et
96Well－Sample－Preparation－for－Suspension－Cells
实验概要The procedure presented below describes a facile method for studying signal transduction events with suspension cells (Jurkat, Raji, THP-1, etc.
转基因
DNA PreparationGene TransferEmbryo TransferTransgenic IdentificatioinOthersTransgenic Outline (University of Michigan Transgenic Animal Model Core)Thi
Preparation－of－High－Titer－Adenovirus－in－P11－cells
adapted from Cell Biology, A Lab Manual, second edition, volume 1 -Grow up P11 cells in 15 cm plates to 70 – 80% confluence. -Infect cells with a MOI
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells1
Homogeneity of cell populations is a prerequisite for the analysis of biochemical and molecular events during male gamete differentiation. Given the c
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells2
Flow Cytometric AnalysisPrior to flow cytometric analysis, the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) was added to cell suspensio
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells3
Moreover, when the method was applied to the analysis of the cellular composition of immature testes, the results were also in agreement with previous
Human－Embryonic－Stem－(ES)－Cell－Protocols——Freezing－Human－ES－Cells
Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up).With a 5 ml pipet, gently pipet and scrape colonies f
Human－Embryonic－Stem－(ES)－Cell－Protocols——Thawing－Human－ES－cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form.Thaw cryovial by gently swirling in waterbath until only a small
Human－Embryonic－Stem－(ES)－Cell－Protocols—Splitting－Human－ES－cells－onMatrige
based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of co
Human－Embryonic－Stem－(ES)－Cell－Protocols——Splitting－Human－ES－cells－on－MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 °C in a water bath.Aspirate media off of cell culture plate.Add the following a
细胞遗传学——染色体
Chromosome Staining and Banding Technique (Primate Cytogenetics Network)Protocols for different staining method, each is in great detail. Karyotype A
GentleMACS/单细胞悬液制备文献集锦
种属 处理组织 文章名称 human
Xenograft－Tumor－Model－Protocol
Preparation of tumor cellsGrow cells in complete medium and exclude any contaminationWhen cells are 70-80% confluent, 3-4 hrs before harvesting, repla
The－OP9DL1－System:－Generation－of－TLymphocytes－from－Embryonic1
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In VitroRoxanne Holmes and Juan Carlos Zúñiga-Pflücker1Sunn
DNA微序列技术
· Protocols for Making Drosophila Arrays (Stanford U.)Detailed protocol for making arrays including PCR Amplification of cDNAs for Printing,
多克隆抗体
Making Antibody· Production of Polyclonal Antibody in Rabbit (Walter Steffen)Provides detailed protocol for immunizatioin, bleeding procedure,
Intestinal－intraepithelial－lymphocytes
Intestinal intraepithelial lymphocytes Intestinal intraepithelial lymphocytes (IELs) are mostly T cells, which are continuously associated with gut