UseofTransmissionElectronMicroscopy
Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented. MaterialZeiss EM9-S transmission electron microscope Procedure1. Familiarize yourself with the basic operations of Fixation, embedding and sectioning of materials for viewing in an electron microscope. 2. Insert and Remove a Specimen from the chamber. 3.......阅读全文
Immunofluorescence－Microscopy－of－tissue－culture－cells
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
Silver:－TimeLapse－Microscopy
Pad Preparation1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages of a
Fixation－of－Cells－Cultured－in－Transwell－Dishes
Fixation of Cells Cultured in Transwell DishesTranswell culture dishes are commonly used to culture cells so that the top and bottom of the cells can
HOW－TO－USE－THE－COULTER－COUNTER－TO－COUNT－CELLS
1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amn
试剂标准分类
优 级 纯 试 剂GR Guaranteed reagent分 析 纯 试 剂AR Analytical reagent化 学 纯 试 剂CP Chemical pure实 验 试 剂LR Laboratory reagent纯Pur Pure Purum特 纯EP Extra Pure特 纯Pur
电子探针仪与扫描电镜有何异同
透射电子显微镜 (transmission electron microscopy﹐简写为TEM)。 构造原理 ： 电子显微镜的构造原理与光学显微镜相似﹐主要由照明系统和成像系统构成(图1 光学显微镜与电子显微镜的对比 )。照明系统包括电子枪和聚光镜。钨丝在真空中加热并在电场的作用下发射出电
Immunofluroescence－Technique
Fix cells in 2% formaldehyde in PBS/pH 7.4 for 15 min. at 20oC. 2% formaldehyde is made up fresh prior to use by dissolving the appropriate amount of
光合电子传递－（photosynthetic－electron－transport）
光合作用中，受光激发推动的电子从 H2 O到辅酶Ⅱ（ NADP ）的传递过程。光合色素吸收光能后，把能量聚集到反应中心——一种特殊状态的叶绿素 a分子，引起电荷分离和光化学反应。一方面将水氧化，放出氧气；另一方面把电子传递给辅酶Ⅱ（ NADP ），将它还原成 NADPH，其间经过一系列中间（电
EBSD分析（electron－backscatter－diffraction）是指
EBSD即电子背散射衍射。EBSD的原理始于20世纪50年代，技术问世于80年代。EBSD是扫描电子显微镜（SEM）的一个标准分析附件，但大大拓宽了扫描电子显微镜进行微观分析的功能。它可以与SEM的其他功能（包括EDS等配件）结合起来，原位成像、成分分析、大样品分析、粗糙表面成像等，克服了传统分析方
免疫细胞化学
Introduction to Immunocytochemistry (House Ear Institute)A brief overview of common available methods. BrDU Immunocytochemistry using peroxidase and
Setup－and－use－of－a－twolaser－multiphoton－microscope
Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imagingDavid Entenberg,1 Jeffrey Wyckoff,1 Bojana Gligori
Basic－Theory－and－Use－of－GCMS（二）
For ionisation to take place at all, chemical reaction between the sample and the reagent gas must be exothermic. The grater the heat of the reaction,
Use－of－the－Bradford－Protein－Assay－in－a－Microtiter－Plate－Format
Introduction The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change in
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
Basic－Theory－and－Use－of－GCMS（一）
BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1. Introduction.2. GC-MS systems and components.3. Vacuum system3.1. Rotary pump3.2. Di
材料形貌分析
相貌分析的主要内容是分析材料的几何形貌，材料的颗粒度，及颗粒度的分布以及形貌微区的成份和物相结构等方面。形貌分析方法主要有：光学显微镜(Opticalmicroscopy,OM)、扫描电子显微镜(Scanningelectron microscopy, SEM)、透射电子显微镜(Transmis
A－semipermanent－mounting－medium－for－immunofluorescence－microscopy
A semi-permanent mounting medium for immunofluorescence microscopyMaterials6gm glycerol2.4gm mowiol6ml distilled water12ml Tris buffer 0.2M at pH 8.5M
第三次生物成像论坛在生物物理所召开
4月8日上午，来自孙飞、朱平、苗龙、徐涛研究组的研究人员和生物成像中心的高级技术人员，在生物物理所召开了第三次生物成像论坛。 会议首先回顾了生物成像中心成功建立300KV Titan Ciros 超高分辨率电镜后所取得的成绩，同时结合近期举办的能量过滤以及STEM培训活动，本
Adventitious－Root－Induction－in－Arabidopsis－thaliana－as－a－Model－for－In...
Adventitious root formation, the development of roots on non-root tissue (e.g. leaves, hypocotyls and stems) is a critical step during micropropag
使用量子级联激光器的可调谐长波红外陷波滤波器的...3
6.Performance Characterization ExperimentThe prototype filters were fabricated based on the device design shown in Fig. 3 and the specifications liste
如何选择电镜：不妨看看这75篇重要文献
这是一篇有关电子显微镜的综述，是根据75篇发表使用实验的文章归纳的。可以帮助读者找到最适合的电子显微镜。日立高新Hitachi High Technologies America为研究碳酸酐酶可通过spidroin蛋白末端功能域促进蜘蛛丝的形成，采用Hitachi的H7100 electr
我国揭示可指导材料精准制备的材料取向连接生长机制
在国家自然科学基金项目（项目编号：51420105003、11327901、11525415等）等资助下，孙立涛教授团队与国际项目合作单位美国伯克利劳伦斯国家实验室的郑海梅团队在材料取向连接生长机理方面取得突破。研究成果以“In-situ Liquid Cell Transmission Ele
Use－of－Nonaqueous－Fractionation－and－Metabolomics－to－Study－Chloroplast－...
Use of Non-aqueous Fractionation and Metabolomics to Study Chloroplast Function in ArabidopsisChloroplasts are the chemical factories of plant cells b
Use－of－the－B.D.－FACS－or－Calibur－Flow－Cytometers
Reservations:Schedule time on a cytometer (FACS or Calibur) by filling in the calendar at the flow lab. Don't grossly overbook since we have to pa
How－to－use－Basic－Local－Alignment－Search－Tool－(BLAST)
DescriptionThe BLAST algorithm was developed as a way to perform DNA and protein sequence similarity searches by an algorithm that is faster than FAST
Size－and－Shape－of－Protein－Molecules1
Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron MicroscopyAn important part of ch
Measurement－of－GFP－Expression－and－DNA－Content－in－Permeabilized－Cells
ReagentsCells to be studied expressing green fluorescent protein (GFP). Note that the same cell type without GFP is needed as a control.1 X PBS2% Buff
《Nature》冉冉升起的新星之中国学者葛炳辉——电子显微镜
创造性思维驱动科学发展，起决定作用的永远是人，最新一期（9月19日）Nature杂志中的“Nature Index 2018 Rising Stars”介绍了在自然科学领域崭露头角的一群研究人员，他们也许正在定义着未来的科学研究。这11位职业生涯早期或中期的科学家所在的领域从削减太阳能电力的成本到降
Low－Loss－Sapphire－Windows－for－High－Power－Microwave－Transmission（七）
The failure data is shown for the 12.5 mm diameter disks is shown in Fig. 12. The average failure pressure for the standard disks is 12 MPa, whe