Setupanduseofatwolasermultiphotonmicroscope
Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imagingDavid Entenberg,1 Jeffrey Wyckoff,1 Bojana Gligorijevic,1 Evanthia T Roussos,1 Vladislav V Verkhusha,1 Jeffrey W Pollard1 & John Condeelis1 Affiliations Contributions Corresponding author Journal name: Nature Protocols Volume:6,Pages:1500–1520 Year published: (2011) DOI: doi:10.1038/nprot.......阅读全文
Setup－and－use－of－a－twolaser－multiphoton－microscope
Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imagingDavid Entenberg,1 Jeffrey Wyckoff,1 Bojana Gligori
USE－OF－THE－LIGHT－MICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjus
体外荧光法检测核内体早期动力学3
REAGENT SETUPCritical step All solutions and buffers must be free of detergent and therefore should be prepared in plasticware, or in glassware thorou
Flow－Cell－Assays－with－Microtubules:－Motility/Dynamics－in－Fluorescence
Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/
Measurement－with－the－Light－Microscope
Measurement with the Light MicroscopeYour microscope may be equipped with a scale (called a reticule) that is built into one eyepiece. The reticule ca
Live－imaging－with－Drosophila－tissue－culture－cells2
Materials & ReagentsDrosophila Schneider S2 cellsSchneiders Medium (GIBCO/Invitrogen), 10% fetal calf serum, Antibiotics (Sigma A5955)Depression slide
Microscopes－in－Cell－Biology
Microscopes in Cell BiologyIntroductionMicroscopy has a major role in the study of cells. From the very beginning, researchers have tried to develop w
Use－of－a－Hemacytometer
A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c
Use－of－Micropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identifie
Transmission－Electron－Microscope－(TEM)
所谓TEM，就是一个放大镜叠加了一台照相机。这台放大镜的放大倍数比较高，可高达一百万倍。当然，抛开分辨率谈放大倍数都是耍流氓，那么，TEM的分辨率有多高呢？答案是 it depends。一般来说，TEM的分辨率要在1到2个纳米，STEM更高，但是STEM得成像技术类似于SEM，但用的不是二次电子。我
Immunofluorescence－Labeling－of－Cells
实验概要Antibodies are an important tool for demonstrating both the presence and the subcellular localization of an antigen. Cell staining is a very ver
体外荧光法检测核内体早期动力学2
Full size image (70 KB)In vitro incubation of a reaction mix that contains labeled endosomes, cytosol and an ATP-regenerating system at physiological
USE－诱变实验
实验材料 带 mutS 表型（例如 BMH71-18) 的转化用大肠杆菌感受态 带 mut+表型的转化用大肠杆菌感受态 试剂、试剂盒
USE－诱变实验
实验材料 带 mutS 表型（例如BMH71-18) 的转化用大肠杆菌感受态带 mut+表型的转化用大肠杆菌感受态试剂、试剂盒 退火缓冲液贮存液合成缓冲液噬菌体 T4DNA 连接酶噬菌体 T4DNA 聚合酶或测序酶单一位点的限制性内切核酸酶琼脂糖凝胶诱变引物选择引物质粒 DNA仪器、耗材 70°C
USE－诱变实验
本方法中，两条寡核苷酸引物杂交到变性重组质粒 DNA 双链的同一条链上。一条引物（诱变引物）携带一个拟引进靶 DNA 序列的突变，第二条引物携带一个能破坏质粒单一限制酶位点的突变。本实验来源于分子克隆实验指南（第三版）下册，作者：〔美〕J. 萨姆布鲁克 D.W. 拉塞尔。实验材料带 mutS 表型（
Fluorescence－Mounting－Medium－(Antifade)
Materials Needed20ml glass scintillation vialSmall stir barFoilGlycerol1X PBSPipets* P-phenylenediamine ( EMD Chemicals Inc. Cat# PX0730)Carbonate-Bic
DAPI－Counterstaining－Protocols
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
Immunofluorecence－P...
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Immunofluorescence－...
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Live－imaging－with－Drosophila－tissue－culture－cells1
IntroductionLive imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It allows follow
DAPI－Nucleic－Acid－Stain
实验概要The blue-fluorescent DAPI nucleic acid stain preferentially stains dsDNA; it appears to associate with AT clusters in the minor groove. Binding
偏光显微镜(Polarizing－microscope)
一、偏光显微镜的特点偏光显微是鉴定物质细微结构光学性质的一种显微镜。凡具有双折射性的物质，在偏光显微镜下就能分辨的清楚，当然这些物质也可用染色法来进行观察，但有些则不可能，而必须利用偏光显微镜。偏光显微镜的特点，就是将普通光改变为偏振光进行镜检的方法，以鉴别某一物质是单折射性（各向同性）或双折射性（
Use－of－Transmission－Electron－Microscopy
Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented. MaterialZe
Use－of－the－GUS－Reporter－Gene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter act
Fluorescence－In－Situ－Hybridization－using－TSA™
实验概要This protocol describes steps for fluorescent in situ hybridization (FISH) to Drosophila embryos using Tyramide Signal Amplification (TSA™), and
How－to－perform－automated－counts－of－fluorescently－stained－cells.
NoteThis protocol describes semi-automated cell counts using fluorescently labeled cells, a hemocytometer and ImageJ software. The hemocytometer is n
Tunel－Procedure－in－Bovine－Embryos－牛胚胎TUNEL检测凋亡
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go highe
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
TUNEL－PROCEDURE－IN－BOVINE－EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high
使用量子级联激光器的可调谐长波红外陷波滤波器的...3
6.Performance Characterization ExperimentThe prototype filters were fabricated based on the device design shown in Fig. 3 and the specifications liste