Silver:TimeLapseMicroscopy
Pad Preparation1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages of agarose pads please let us know, bruno)2. Apply 2mL to a 24x60 mm coverslip.3. Cover with additional coverslip, creating a sandwich. Let cool for 30 minutes. (1h seems to work better)Cell Preparation4. Grow 5mL culture in Thorn media to OD 600 = 0.3. Back dilute if neces......阅读全文
Silver:－TimeLapse－Microscopy
Pad Preparation1. Microwave 2% agarose (mix of low-melt and normal, to taste) in Thorn media (see below). (If you have used different percentages of a
Silver－Enhancement－...
实验概要The method provides a silver enhancement protocol for immunoassay.主要试剂Prepare the following reagents fresh daily except for the citrate buffer.1.
Silver:－Lysate－for－Western
Grow cellsHarvestSpin downWash with PBSSpin down enough cells to collect a 50-100 µL cell pellet in a FastPrep tubePrepare lysis buffer (beforehand) a
Silver:－Colony－PCR
Zymolyase Solution:Regular stock of zymolyase is 10 mg/ml diluted in water, mix by inverting, not all will go into solution (filter it) store aliquots
Silver－Staining－Protocol
1x 40min - overnight 50% MeOH, 12% Acetic Acid1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde3x 20min
ELECTRON－MICROSCOPY
E.M. PROCESSING SCHEDULE - EPOXY RESINFix tissue in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer at 4oC, for a minimum of 4 hours. Tissue shou
Light－Microscopy
The light microscope, so called because it employs visible light to detect small objects, is probably the most well-known and well-used research tool
Silver－Acetate－Autometallography－(AMG)
In the early eighties, a series of papers were published by Gorm DANSCHER, Aarhus, Denmark, to introduce a reliable and easy-to-handle technique for l
Phase－Contrast－Microscopy
Phase Contrast MicroscopyPrincipleMost of the detail of living cells is undetectable in bright field microscopy because there is too little contrast b
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Immunofluorescence－Microscopy－Protocol
实验概要Immunofluorescence allows the imaging of a specific factor in cells or tissue sections through the use of a specific antibody chemically which i
Microscopy－with－Oil－Immersion
Microscopy with Oil ImmersionPrincipleWhen light passes from a material of one refractive index to material of another, as from glass to air or from a
SSR－GEL－and－Silver－Staining－Protocol
I. EQUIPMENT:DNA sequencing unit (35 x 45 cm) & 2000V power supplyClampsLg. plastic trays (4), about 43 x 50 x 8 cm, and one lidTwo rocking platformsH
Immunofluorescence－/－Confocal－Microscopy－Protocol
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Immunofluorescence－/－Confocal－Microscopy－Protocol
实验概要Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on biological samples. This tec
Generic－Fixation－for－Electron－Microscopy
Generic Fixation for Electron MicroscopyThe best way to fix a sample for electron microscopy is to follow a procedure developed and proven by others.
Use－of－Transmission－Electron－Microscopy
Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented. MaterialZe
Live－imaging－with－Drosophila－tissue－culture－cells1
IntroductionLive imaging provides an important complementation to the "snapshot" view obtained in fixed tissue by immunofluorescence. It allows follow
Chlamydomonas－Fixation－for－Transmission－Electron－Microscopy
Chlamydomonas Fixation for Transmission Electron MicroscopySolutions:Chlamydomonas culture medium + 2% glutaraldehyde (5 ml medium + 0.9 ml 25% glutar
Specimen－Preparation－for－Scanning－Electron－Microscopy
Specimen Preparation for Scanning Electron MicroscopyWe recommend consultation with one of the lab directors before preparing specimens. The methods p
Tetrahymena－Fixation－for－Transmission－Electron－Microscopy
Tetrahymena Fixation for Transmission Electron MicroscopyPellet Tetrahymena cells in a clinical centrifuge.OPTIONAL: Suspend cells in HNMK (50 mM HEPE
Negative－Stain－Electron－Microscopy－of－Microtubules
Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of
Immunofluorescence－Microscopy－of－tissue－culture－cells
Immunofluorescence Microscopy of tissue culture cellsThese methods are written for direct staining of filamentous actin with bodipy FL-phallicidin and
Fixation－and－Embedding－of－Microtubules－for－Electron－Microscopy
(This procedure can also be used for virtually any material that must be pelleted prior to fixation and thin sectioning)Primary fix:2% glutaraldehyde
SDI检测仪XFsilver
SDI检测仪/污染指数仪/污染指数测定仪 型号：XF-silver 指数（SDI）值，也称之为FI（Fouling Index）值，是水质指标的重要参数之一。它代表了水中颗粒、胶体和其他能阻塞各种水净化设备的物体含量。通过测定SDI值，可以选定相应的水净化技术或设备。 在反渗透水处理
SDI检测仪XFsilver
SDI检测仪/污染指数仪/污染指数测定仪 型号：XF-silver 指数（SDI）值，也称之为FI（Fouling Index）值，是水质指标的重要参数之一。它代表了水中颗粒、胶体和其他能阻塞各种水净化设备的物体含量。通过测定SDI值，可以选定相应的水净化技术或设备。 在反渗透水处
银染(silver－staining)操作规程
实验原理：在碱性条件下，用甲醛将蛋白带上的硝酸银（银离子）还原成金属银，以使银颗粒沉积在蛋白带上。染色的程度与蛋白中的一些特殊的基团有关，不含或者很少含半胱氨酸残基的蛋白质有时候呈负染。银染的详细机制还不是非常清楚。 试剂：乙醇、冰醋酸、乙酸钠、硫代硫酸钠、硝酸银、碳酸钠、甘氨酸或EDTA.Na
免疫电镜(Immune－electron－microscopy)原理
（一） 原理 免疫电镜技术是免疫化学技术与电镜技术结合的产物，是在超微结构水平研究和观察抗原、抗体结合定位的一种方法学。它主要分为两大类：一类是免疫凝集电镜技术，即采用抗原抗体凝集反应后，再经负染色直接在电镜下观察；另一类则是免疫电镜定位技术。该项技术是利用带有特殊标记的抗体与相应抗原相结合，在电
免疫电镜(Immune－electron－microscopy)原理
（一） 原理免疫电镜技术是免疫化学技术与电镜技术结合的产物，是在超微结构水平研究和观察抗原、抗体结合定位的一种方法学。它主要分为两大类：一类是免疫凝集电镜技术，即采用抗原抗体凝集反应后，再经负染色直接在电镜下观察；另一类则是免疫电镜定位技术。该项技术是利用带有特殊标记的抗体与相应抗原相结合，在电子
EPON－resin－mixture－for－transmission－electron－microscopy
EPON resin mixture for transmission electron microscopyFor Epon WPE 153:~120 ml~60 ml~30 mlMix A:Embed 81244 ml22.1 ml11.1 mlDDSA67 ml33.3 ml16.7 mlMi