USEOFTHELIGHTMICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjust the iris diaphragm and focus the condenser to give its optimum performance, this could result in objects in the preparation being undetected.There are many microscope set up procedures for specific functionality but the most versatile for general use is ‘Critical Illu......阅读全文
Double－immunofluore...
实验概要We provide a protocol for immunofluoresent double staining incubating the antibodies together.In order to be able to examine the co-distributi
Dynamic－Monitoring－ofCellular－Remodeling－Induced－bythe－Transforming－Growth1
The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of se
Fluorescence－Procedures－fortheActin－andTubulin－Cytoskeleton－in－Fixed－Cells2
Formaldehyde FixationFix in 4% formaldehyde (16% stock EM grade) in CBS for 20 minutesRinse in TBSPermeabilize as for methanol fixationProcede as for
Axolotl－Microsurgery
I. Embryo Preparation1) Manually dejelly embryo of appropriate stage. Use fine foreps to break hole in hard jelly coat. Remove membrane around embryo
第21届国际光散射年会将于2010年10月在美国召开
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Science发表：癌细胞移动－神经发育－新技术都能“看得到”
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Bradford－protein－assay
Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly
哺乳动物RNAi技术－Mammalian－RNA－Interference
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York Excerpted from RNAi: A Guide To Gene S
加拿大对中国台湾产登山索Simond－Toucan－Light实施召回
2019年8月7日，加拿大卫生部宣布对中国台湾产登山索实施召回。 此次召回产品为Simond Toucan Light的登山索。货号为2575753，铝体为蓝色，上面印有操作图、兼容性等信息，以及“Simond Toucan Light”。 该产品在使用时，有可能被卡住。 该产品于2018
重新打开生物学大门：light－sheet晶格层光显微镜
在生物学界，对活体细胞的观察至关重要。所以曾经有人提出过拍摄正在运动的细胞图片，但技术上无法达到要求。 现在，诺贝尔化学得主Eric Betzig的团队宣布：继手掌显微镜之后，又研发出一种新型的光学显微镜，被命名为“晶格层光显微镜”。由于晶格层光显微镜的出现，传统光学显微镜所遇到的难题就迎刃而解了
TEM－Specimen－Preparation：Preparative－Techniques－for－the－TEM
For routine transmission electron microscopy (TEM), it is generally accepted that specimens should be thin, dry and contain molecules which diffract e
Nucleolar－Isolation－Protocol2
7. Resuspend the pellet with 3 ml S1 solution (Figure 3). The pellet should be resuspended readily by pipetting up and down. A pellet that cannot be r
viral－immunity－－pathogenesis－group
OUTLINEWe consider manual counts as less quick but more precize than authomatic (electronic) since in the latter case the counts are based on the size
Coenzyme－A－Detection
实验概要The experiment provided an easy, convenient assay to measure the CoA level in a variety of biological samples. In the assay, free CoA is specif
LIVE/DEAD®－Fixable－Dead－Cell－Stain－Kits
实验概要The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are
Flow－Cytometry－Analysis
PurposeFlow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide r
Fluorescent－Nucleoside－Triphosphates－for－SingleMolecule－Enzymology2
1.1 Selection of the Labeling PositionFluorescent adenine and guanine nucleotides have been widely used to report upon binding, protein release and
Em－observations－of－microsomes
Em observations of microsomesLEVEL I Figure 7.3 Isolated microsomes Figure 7.4 TEM of hepatocyteMATERIALS1% Glutaraldehye (GTA)1% Osmium tetroxideEpox
Microtubule－(MT)/Organelle－Motility－Assays
Rapidly thaw and immediately place on ice one aliquot each of axonemes, Golgi or ER membranes, 45 uM tubulin, rat liver cytosol, and 20x energy regene
Light－|－AI+椭偏仪：从“众里寻它”到“灯火阑珊”
薄膜材料在微电子、光电子、生物、医药等领域高端器件的制造中不可或缺。为了提高器件的性能指标，人们需要精确地测量与掌握薄膜的特性参数，比如光学常数(n, κ)和薄膜厚度(d)就是两种重要的材料参数。前者同材料组分、带隙、结晶度等信息密切相关，而薄膜厚度的微小误差会对器件性能的造成显著影响。椭偏仪因
AbC™－AntiMouse－Bead－Kit
实验概要The AbC™ Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when u
AbC™－AntiMouse－Bead－Kit
实验概要The AbC™ Anti-Mouse Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when u
抗生素溶液配制
Working concentrations and stock solutionsPreparation of stock solutionsAmpicillinCarbenicillinGE antibiotic mixTetracyclineChloramphenicol Supplement
Preparation－Of－Ciliated－Protozoa－For－Scanning－Electron－Microscopy
Preparation Of Ciliated Protozoa For Scanning Electron MicroscopyGeneral notes: The same procedures are used to fix and stain cells for SEM and for TE
免疫细胞化学
Introduction to Immunocytochemistry (House Ear Institute)A brief overview of common available methods. BrDU Immunocytochemistry using peroxidase and
Lysosome－Isolation－in－Isotonic－Sucrose
LEVEL IMATERIALSRat liverPhysiological saline (0.85% w/v NaCl)0.25 M sucrose in 10 mM Tris-HCL, pH 7.4Brendler teflon homogenizerRefrigerated preparat
Studier－Lysate－Prep
SummaryHow to make a lysate from a plaque preparation. We also use this protocol for preparation of a quick stock from previously made lysate prep.Pro
The－UnderAgarose－Migration－Assay2
F. Video Microscopy 1. The behavior of migrating cells may be filmed with an inverted microscope fitted with a CCD camera. 2. Determine the best magni
Appendix－G:－Spectrophotometry——2
ABSORPTION SPECTRUM:Analysis of pigments often requires a slightly different use of a spectrophotometer. In the use of the instrument for determinatio
Immunohistochemistr...
实验概要Peprotech provides a immunohistochemistry protocol for Mouse Anti-Human MCP-1*.实验步骤The following protocol used formalin-fixed paraffin-embeddedhum