USEOFTHELIGHTMICROSCOPE

USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjust the iris diaphragm and focus the condenser to give its optimum performance, this could result in objects in the preparation being undetected.There are many microscope set up procedures for specific functionality but the most versatile for general use is ‘Critical Illu......阅读全文

In-Situ-Hybridization-to-Somatic-Chromosomes-in-Drosophila

In Situ Hybridization to Somatic Chromosomes in DrosophilaAbby F. DernburgINTRODUCTIONIn situ hybridization was originally developed as a technique fo

AbC™-AntiRat/Hamster-Bead-Kit

实验概要The AbC™  anti-Rat/Hamster Bead Kit provides a consistent, accurate, and  simple-to-use technique for the setting of flow cytometry compensation  

AbC™-AntiRat/Hamster-Bead-Kit

实验概要The AbC™  anti-Rat/Hamster Bead Kit provides a consistent, accurate, and  simple-to-use technique for the setting of flow cytometry compensation  

欧盟发布奇亚(欧鼠尾草)作为食品的安全性意见

  2019年4月17日,应欧盟委员会要求,欧盟膳食、营养和过敏症科学小组(NDA)就奇亚(欧鼠尾草) (Chia Seed Salvia Hispanica L. )作为新型食品的安全性发布意见。图片来源于网络  经过评估,专家小组得出结论,在当前评估的使用条件下,使用奇亚是安全的。  部分原文报

欧盟发布奇亚(欧鼠尾草)作为食品的安全性意见

  2019年4月17日,应欧盟委员会要求,欧盟膳食、营养和过敏症科学小组(NDA)就奇亚(欧鼠尾草) (Chia Seed(Salvia Hispanica L. )作为新型食品的安全性发布意见。图片来源于网络  经过评估,专家小组得出结论,在评估的使用条件下,使用奇亚是安全的。  部分原文报道如

Dynamic-Flow-Assay-in-a-Parallel-Plate-Flow-Chamber

Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850Flow assays allow visualization of c

Fluorescence-Procedures-forthe-ActinandTubulin-Cytoskeleton-inFixed-Cells2

Actin CytoskeletonMethanol fixationFix in -20oC methanol for 1-2.5 minutesRinse in TBSPermeabilize in TBS-0.5% TX for 10 minutesRinse in TBS-0.1% TX (

Invitro-Phagocytosis-Assay-of-Macrophages

IntroductionThe term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular pr

LaVision双光子显微镜多线扫描双光子成像(三)

2.2.多线TPLSM中通过成像检测释放光    在单光束TPLSM中,光电倍增管PMT或者雪崩二极管APD可以很方便地用于释放光检测,由于双光子激发的原理,激发只发生在激光焦点处。因此,用于屏蔽离焦光线的共焦小孔变得不必要,并且可以使用NDD检测。这意味着激发光不会被送回扫描镜,而是直接进入位于靠

Immunoprecipitation...-(一)

实验概要We provide a  general IP procedure including a list of reagents and a table to help  you choose the correct protein beads.Immunoprecipitation is a

Derivation-and-Culture-of-Dopaminergic-Neurons-(from-Midbrains-of-Rodents)

实验概要Dopaminergic  (DA) neurons are located in the ventral midbrain (VM). The ability to  isolate precursor cells and neurons from the VM provides a po

Detection-of-apoptotic-process-in-situ-using-immunocytochemical

1. INTRODUCTION  Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pathological

Guidelines-for-theUse-of-Analgesics-and-Tranquilizers-in-Laboratory-Animal7

ThermoregulationAnimals frequently become hypothermic during anesthesia because of inhalation of cold gases, exposure of body cavities to the room air

Tissue-Culture-Methods3

REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach student should m

典型陆地生态系统碳收支对光的响应研究获进展

  光是陆地生态系统碳循环的驱动因子。由于气候变化引起的降水格局的改变、以及人类活动引起的大气气溶胶的变化使云量格局、区域的太阳辐射条件产生了相应的变化,从而使陆地生态系统碳收支对光的响应研究成为陆地生态系统碳循环环境控制机制研究方面关注的热点问题。   在国家重点基础研究发展计划“

Cell-counting-with-an-hemacytometer.

Accuracy of manual counts with an hemacytometer depend on:accurate mixing of the sample (no bubbles!)number of chambers countednumber of cells counted

TUNEL-PROCEDURE-IN-BOVINE-EMBRYOS

Materials8% (w/v) paraformaldehyde stock solution:  Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high

Tunel-Procedure-in-Bovine-Embryos-牛胚胎TUNEL检测凋亡

Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go highe

TUNEL-PROCEDURE-IN-BOVINE-EMBRYOS

Materials8% (w/v) paraformaldehyde stock solution:  Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go high

A-semipermanent-mounting-medium-for-immunofluorescence-microscopy

A semi-permanent mounting medium for immunofluorescence microscopyMaterials6gm glycerol2.4gm mowiol6ml distilled water12ml Tris buffer 0.2M at pH 8.5M

In-Vivo-Imaging-of-Far1

In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle TissueDNA electrotransfer to muscle tissue yields long-term, high

Isolation-of-kidney-glomeruli-from-mice

Isolation of mice glomeruli1. Mice were anesthetized by an intraperitoneal injection of Avertin (2,2,2-tribromoethyl and tertiary amyl alcohol; 17

Fluorescent-Nucleoside-Triphosphates-for-SingleMolecule-Enzymology1

By: Christopher P. Toseland1 2 , Martin R. Webb1Affiliation(s): (1) MRC National Institute for Medical Research, London, UK(2) Institut für Zelluläre

MITOMYCIN-C-TREATMENT-OF-PMEFs

Cultures to be treated should be sub confluent ie actively growing.1. Add 1/20 volume Mitomycin C (200 ug/ml 10 ug/ml), to culture and incubate at 37

Development-of-the-Heart-Morphogenetic-Field-in-the-Axolotl-Embryo

IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative d

普鲁兰混标试剂使用说明书

Cat No. 中文名称 Name Mw Mp Mn 规格 34037060 普鲁兰混标套装 Pullulan KIT  

ELISPOT-(Enzymelinked-ImmunoSPOT)-实验方法步骤2

Cytokine ELISPOT ProtocolDescriptioneBioscience ELISPOT Ready-SET-Go! reagent sets contain the necessary reagents for performing enzyme linked immunos

Flow-Cell-Assays-with-Microtubules:-Motility/Dynamics-in-Fluorescence

Flow cell assays are very useful for studying microtubule motility, microtubule dynamics, kinetochore-microtubule interactions and action of severing/

Fibroblast-Cell-Systems3

Seeding After cells are thawed:NOTE: Do not dispense the entire contents of the cryovial into one T-25 flask!!Remove the cap, being careful not to tou

葡萄糖半固体原理及结果(动力试验)

Motility: Semi-Solid Motility Medium Purpose The movement of bacteria is detected by use of a medium with low agar concentration. The medium is in