USEOFTHELIGHTMICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjust the iris diaphragm and focus the condenser to give its optimum performance, this could result in objects in the preparation being undetected.There are many microscope set up procedures for specific functionality but the most versatile for general use is ‘Critical Illu......阅读全文
体外荧光法检测核内体早期动力学3
REAGENT SETUPCritical step All solutions and buffers must be free of detergent and therefore should be prepared in plasticware, or in glassware thorou
Effect－of－UV－Treatment－on－Early－Development－of－Sea－Urchin－Embryos
Objective:To test the effects of UV radiation at 254nm on Sea Urchin embryo development after radiation at the 2-cell stage.Background:Sea Urchins exh
DNA－EXTRACTION－FROM－MICRODISSECTED－PARAFFIN－SECTIONS
This is a four day procedure so it's best to start on Monday or Tuesday.CASE SELECTION:H&E stained thin sections are first reviewed by a pathologi
Streptomyces:Protocols/Spore－Prep
Spore Prep - Inoculating & HarvestingDescription A spore prep is a method of preserving a sporulating strain of Streptomyces. The stock is stored in 2
Guidelines－for－Retroorbital－Bleeding－in－Laboratory－Rats－and－Mice
Materials Needed:Micro pipettes (l00 µl) or Pasteur pipettes drawn to fine tip2X2 gauze squaresNon-sterile glovesEppendorf or other tubes to hold samp
DNA转化实验指导5
2C. Notes on Transformation 1. Bacto tryptone and yeast extract can cause allergic reactions. 2. All containers used to handle the bacteria (
How－to－interrupt－scintillation
Peter Novick Lab, Department of Cell Biology Yale University School of Medicine HOW TO PERFORM AN INTERRUPT OF ANONGOING AUTOCOUNT1). This program int
ORNL－MICROARRAY－HYBRIDIZATION－PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5:A. MATERIALSRNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat #
Matrigel－invasion－assays
OverviewMatirgel is considered as basement membrane and generated from EHS sarcoma. Matrigel contains not only basement membrane components (collagens
Immunofluorescence－Whole－Mount
Objective:Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically
Handling－Fresh－Hepatocytes－in－Suspension
实验概要Important—Please read!GIBCO® Fresh Hepatocytes are shipped in cold preservation medium designed to keep cells viable at 4°C. This shipping mediu
3HThymidine－Uptake－by－Cultured－Cells
3H-Thymidine Uptake by Cultured Cells OverviewA radioactive method for measuring cultured cell growth with 3H-thymidine is described here. MaterialFi
Vybrant®－DyeCycle™－Violet－Stain
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of phy
Simplified－Arabidopsis－Transformation－Protocol
(Brief version for those who are familiar with the method)Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign.Our present proto
Peripheral－blood－“endothelial－progenitor－cells”
EPC Isolation and Characterization1. EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
Vybrant®－DyeCycle™－Green－and－Orange－Stains
实验概要Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of ph
显微镜技术——电子显微技术
The Transmission Electron Microscope (TEM) (HEI)An explanation of how the TEM works. TEM Specimen Preparation (HEI) Serial Sectioning (Walter Steffe
新技术：InCell－Western－Assay
In-Cell Western AssayComplete Sample Protocol Detailing the SeedingStimulation, and Detection of the HeLa CellularResponse to Epidermal Growth FactorI
Labeling－Tubulin－and－Quantifying－Labeling－Stoichiometry2
II. Labeling ProtocolThe procedure described below can be scaled down if desired. It is essential to perform all steps involving caged dyes under a sa
Tissue－preparation－protocol－for－ChIP
实验概要This protocol describes how chromatin is prepared from tissue, which can subsequently be used for chromatin immunoprecipitation (ChIP). It is re
Fluidigm公司微液流芯片在单细胞研究中的应用（一）
Nature杂志在2009年5月7日的主页文章中大篇幅地介绍了美国Fluidigm公司的微液流芯片在单细胞表达中的应用。A closer look at the single cellMegan ScudellariNature Reports Stem CellsPublished online:
Study－on－a－TwoDimensional－Scanning－MicroMirror－and－Its－Application－...1
Study on a Two-Dimensional Scanning Micro-Mirror and Its Application in a MOEMS Target DetectorChi Zhang,* Zheng You, Hu Huang, and Guanhua LiAuthor i
Alanine－Transaminase－Activity－Assay
实验概要Alanine Transaminase (ALT) is a transaminase (EC 2.6.1.2) also called serum glutamic pyruvic transaminase (SGPT). Alanine Transaminase is found
mpulsive－Pressurization－of－Neuronal－Cells－for－Traumatic－Brain－Injury－Study
实验概要A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic brain injur
Western－blotting样品准备
实验概要Preparation of lysis buffers, protease and phosphatase inhibitors, lysate from cell culture, lysate from tissues, protein concentration, samples
ELISPOT－Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
ELISPOT－Protocol
实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on t
通过细胞受体代谢生物素化进行图像分析
Metabolic biotinylation of mammalian cell receptors for imagingBakhos A. Tannous , btannous@hms.harvard.edu, Massachusetts General Hospital and Harvar
Optimized－Method－for－the－Preparation－of－Rodent－Testicular－Cells3
Moreover, when the method was applied to the analysis of the cellular composition of immature testes, the results were also in agreement with previous
Red－Blood－Cell－Lysis－Protocols
实验概要BioLegend’s Red Blood Cell (RBC) Lysis Buffer (Cat. No. 420301) has been designed, formulated, and tested to ensure optimal lysis of RBCs in sin