HowtouseBasicLocalAlignmentSearchTool(BLAST)
DescriptionThe BLAST algorithm was developed as a way to perform DNA and protein sequence similarity searches by an algorithm that is faster than FASTA but considered to be equally as sensitive. Both of these methods follow a heuristic (tried-and-true) method that almost always works to find related sequences in a database search, but does not have the underlying guarantee of an optimal ......阅读全文
USE－诱变实验
实验材料 带 mutS 表型（例如BMH71-18) 的转化用大肠杆菌感受态带 mut+表型的转化用大肠杆菌感受态试剂、试剂盒 退火缓冲液贮存液合成缓冲液噬菌体 T4DNA 连接酶噬菌体 T4DNA 聚合酶或测序酶单一位点的限制性内切核酸酶琼脂糖凝胶诱变引物选择引物质粒 DNA仪器、耗材 70°C
USE－诱变实验
本方法中，两条寡核苷酸引物杂交到变性重组质粒 DNA 双链的同一条链上。一条引物（诱变引物）携带一个拟引进靶 DNA 序列的突变，第二条引物携带一个能破坏质粒单一限制酶位点的突变。本实验来源于分子克隆实验指南（第三版）下册，作者：〔美〕J. 萨姆布鲁克 D.W. 拉塞尔。实验材料带 mutS 表型（
USE－OF－THE－LIGHT－MICROSCOPE
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多国科学家合作首次创建氢分子波函数平方图像
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How－to－build－a－BAC－library
Introduction The most important aspect of our cloning vectors is that they are based on the E. coli F-factor replicon. It allows for strict
Cells－and－Molecules－involved－in－local－acute－inflammatory－response
Inflammation has several distinct components, including the localized response at the site of tissue injury or infection. Tissue injury stimulates the
Bradford－protein－assay
Bradford protein assayConsiderations for useThe Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly
TLP基因的表达模式分析
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Basic－Protein－Chemistry－Techniques
Coomassie Blue Stain: (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2O. 2) Add 0.5 grams of Coomassie Blue. 3) Just before use, add 50 ml acet
Basic－Methods－of－Culturing－Drosophila
实验概要Basic Methods of Culturing Drosophila实验步骤Stockkeeping1. Mechanics Most stocks can be successfully cultured by periodic mass transfer of a
Basic－Protein－Chemistry－Techniques
实验概要Basic Protein Chemistry Techniques实验步骤Coomassie Blue Stain: (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2O. 2) Add 0.5 grams of Coomassi
Use－of－Transmission－Electron－Microscopy
Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented. MaterialZe
Use－of－the－GUS－Reporter－Gene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter act
How－Progesterone－Initiates－Oocyte－Membrane
Progesterone (Pg) binds to both intracellular iPR and plasma membrane- bound mPR. (Right Top) After binding to Pg, iPR is recruited to the membrane as
How－to－Make－Simple－Solutions－and－Dilutions
1. Simple Dilution (Dilution Factor Method based on ratios)A simple dilution is one in which a unit volume of a liquid material of interest is combine
学术论文常用句型汇总－再也不愁SCI了！
Beginning 1. In this paper, we focus on the need for 2. This paper proceeds as follow. 3. The structure of the paper is as follows. 4. In this
Thermo－Retention－Time－Alignment软件功能介绍（二）
色谱柱进行切割之后， 在 GC 条件保持不变的情况下，对正癸烷纯品进行二次数据采集， 数据结果如图 7 所示：将上述操作得到的两组数据代入到软件中， 由软件自动计算色谱柱切割之后的相关信息， 计算结果如图 8 所示：将上述软件计算结果通过 GC 色谱柱设置软件图 9， 输入到 GC 系统里， 进行化
Thermo－Retention－Time－Alignment软件功能介绍（一）
概述Thermo Retention Time Alignment 软件可以保证 TRACE1300系列气相色谱，在更短的时间内获得更佳的分析质量，达到事半功倍的效果。 它可以消除色谱的变化带来的保留时间的偏离， 同时可以使不同系统之间和不同次进样之间的保留时间得以重现。 此软件将使任意一台TR
DNA的酶学操作
DNA的酶学操作DNA Modifying Enzymes (Michael Blaber)Introduction to bacterial restriction/modification system. It provides very useful background knowledge
Basic－Fluorescent－in－situ－Hybridization－(FISH)
实验概要Fluorescence in situ hybridization method is a kind of physical map drawing method, use fluorescent element mark probe, to detect probe and spli
Basic－Method－for－Indirect－Immunofluorescence－Labeling
Basic Method for Indirect Immunofluorescence LabelingBackgroundThis is the method for indirect immunofluorescence labeling; that is, the antibodies do
筛分仪－AS－200－basic简介
德国RETSCH（莱驰）的AS200系列分析筛分仪广泛应用于科研与开发，原材料、半成品和成品的质量控制及生产监控等领域。其可控电磁驱动能为每一产品提供最优的配置。尖锐样品也能在短时间内过筛。AS200basic具有RETSCH 产品一贯的可靠性和质量保证。该机型采用模拟制式下的振幅和筛分时间设置。所
CREATION－AND－USE－OF－YOUR－INFECTIOUS－VECTOR
实验概要CREATION AND USE OF YOUR INFECTIOUS VECTOR实验步骤Day 1 1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scal
Creation－and－Use－of－Infectious－Virus－Vector
Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired).The f
Use－an－IgG－antiIgM...
实验概要This method involves coupling an IgG anti-IgM antibody to protein A or G beads (see method below). These beads can then be used in the normal
Fluidigm公司微液流芯片在单细胞研究中的应用（二）
It was also worth it for Tannishtha Reya, who studies stem cell fate and cancer at Duke University Medical Center in Durham, North Carolina. "Im
Online－Lectures－on－Bioinformatics
Biological preliminariesAnswersIntroductionReferencesExercisesAnalysis of individual sequencesIntroductionHelical wheels, amphipatic helices and coile
Phosphoamino－acid－analysis:Mark－Kampss－method
Phosphoamino acid analysis:Mark Kamps's method1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis
PCR－Primer－Design（一）
Molecular Biology Today 2001. 2(2): 27-32. Vinay K. Singh and Anil Kumar Bioinformatics Sub-centr
How－do－you－synthesize－your－dsRNA
We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends (I. Primer Desig