UseoftheGUSReporterGene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter activity is simplified in the majority of cases by the use of a readily detectable reporter gene. Indeed, reporter genes can also be used to aid the isolation of promoters, through the transformation of plants with a promoterless reporter gene construct. Activation o......阅读全文
Use－of－the－GUS－Reporter－Gene
One of the most important considerations in the expression of heterologous proteins in plants is the choice of promoter. The study of promoter act
Leaf－GUS－Assay
实验概要a protocol for Leaf GUS Assay This protocol is for small samples (usually single leaf from 21DAI plants), scale up for larger samplesAs there are
Leaf－GUS－Assay
一、实验试剂 GUS Buffer (500 ml) 2.0478 g Na2HPO4 1.2688 g NaH2PO4 (=50 mM NaPi pH7.0) 10 ml 0.5 M EDTA (=10 mM) 0.5 g Triton X-100 0.5 g N-L
Use－of－Micropipets
A. Gilson-style Pipetmen Micropipets (and similar models)We primarily use the Gilson micropipets in the core course labs. We have four sizes identifie
Use－of－a－Hemacytometer
A hemacytometer (also spelled hemocytometer) is an etched glass chamber with raised sides that will hold a quartz coverslip exactly 0.1 mm above the c
USE－诱变实验
实验材料 带 mutS 表型（例如BMH71-18) 的转化用大肠杆菌感受态带 mut+表型的转化用大肠杆菌感受态试剂、试剂盒 退火缓冲液贮存液合成缓冲液噬菌体 T4DNA 连接酶噬菌体 T4DNA 聚合酶或测序酶单一位点的限制性内切核酸酶琼脂糖凝胶诱变引物选择引物质粒 DNA仪器、耗材 70°C
USE－诱变实验
实验材料 带 mutS 表型（例如 BMH71-18) 的转化用大肠杆菌感受态 带 mut+表型的转化用大肠杆菌感受态 试剂、试剂盒
USE－诱变实验
本方法中，两条寡核苷酸引物杂交到变性重组质粒 DNA 双链的同一条链上。一条引物（诱变引物）携带一个拟引进靶 DNA 序列的突变，第二条引物携带一个能破坏质粒单一限制酶位点的突变。本实验来源于分子克隆实验指南（第三版）下册，作者：〔美〕J. 萨姆布鲁克 D.W. 拉塞尔。实验材料带 mutS 表型（
USE－OF－THE－LIGHT－MICROSCOPE
USE OF THE LIGHT MICROSCOPEEach time the microscope is to be used it should be set up correctly to give a good image. Most often users forget to adjus
Protein－Immunolocalization－in－Maize－Tissues
The analysis of gene expression at transcript and at protein level is of outstanding importance in plant developmental biology. Proteins can be loc
gus基因的检测方法
根据gus基因检测所用的底物不同，可以选择三种检测方法：组织化学法、分光光度法和荧光法（灵感度为分光光度检测法最高）。其中最为常用的是组织化学法。组织化学法检测以5-溴-4-氯-3-吲哚-β-葡萄糖苷酸（X-Gluc）作为反应底物，将被检材料用含有底物的缓冲液浸泡。若组织细胞发生了gus基因的转化，
Use－of－Transmission－Electron－Microscopy
Use of Transmission Electron MicroscopyOverviewA protocol describing the use of Zeiss EM9-S transmission electron microscopy is presented. MaterialZe
A－Yeast－Secretion－Trap－Assay－for－Identification－of－Secreted－Proteins－...
Secreted proteins from plants and phytopathogens play important roles in their interactions and contribute to elaborate mechanisms of attack, defe
Creation－and－Use－of－Infectious－Virus－Vector
Creation and use of your infectious vector:Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired).The f
CREATION－AND－USE－OF－YOUR－INFECTIOUS－VECTOR
实验概要CREATION AND USE OF YOUR INFECTIOUS VECTOR实验步骤Day 1 1. Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scal
Use－an－IgG－antiIgM...
实验概要This method involves coupling an IgG anti-IgM antibody to protein A or G beads (see method below). These beads can then be used in the normal
HOW－TO－USE－THE－COULTER－COUNTER－TO－COUNT－CELLS
1) Turn on the counter by pulling out the on/off button. You need to do this at least 10 min before use to obtain sufficient vacuum.Usually put 0.2 ml
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amn
基因转型(gene－transformation)
目的带有特定基因的质体在分子生物研究上，是一个很重要的工具，将质体送入细菌的过程称为基因转形，经由基因转型可使质体在细菌中大量复制，以备进一步研究。本实验将把你在前面转殖实验中cDNA和质体DNA的连结反应送入细菌。 你将从本实验学习如何进行基因转型作用。原理早在1970年左右，有人发现细菌经由冰冷
Gene－Structure－Annotation－at－PlantGDB
The accurate identification of exons and introns that comprise a complete plant gene structure can be a time-consuming and challenging task. Novel
生物分子学中GUS什么意思
应该是指的GUS基因，在植物样品中很常用的一个报告基因。GUS基因就是β-葡萄糖苷酸酶基因 。该基因产物为β-葡萄糖苷酸酶，为一水解酶。可使用市售的底物 (substrate) 在原位 (in situ) 显现出酶的活性，以肉眼即可检测其产物，非常方便。
转基因植物的GUS表达分析
实验概要本实验对转基因拟南芥进行了GUS组织化学染色分析及GUS活性测定。主要试剂X-Gluc，丙酮，乙醇，BSA，考马斯亮蓝溶液主要设备显微镜(BX50 OLYPUS)，研钵，高速离心机，Nano drop核酸蛋白测定仪实验材料转基因拟南芥实验步骤1. 转基因植物的GUS组织化学染色分析 1)
登革热基孔肯雅热三合一核酸检测试剂盒英文使用说明
Lot-No.Ref. FR342 Expiry time: 1 year100 Tests (Ready to use PCR kit) -Only for in vitro use--Only for research use – To be used by a technical pers
病毒核酸检测试剂盒英文使用说明
Lot-No.Ref. FR340 Expiry time: 1 year100 Tests (Ready to use PCR kit) Zika Virus (Real time) DOUBLE CHECK
Basic－Theory－and－Use－of－GCMS（二）
For ionisation to take place at all, chemical reaction between the sample and the reagent gas must be exothermic. The grater the heat of the reaction,
Use－of－the－Bradford－Protein－Assay－in－a－Microtiter－Plate－Format
Introduction The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change in
Setup－and－use－of－a－twolaser－multiphoton－microscope
Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imagingDavid Entenberg,1 Jeffrey Wyckoff,1 Bojana Gligori
Basic－Theory－and－Use－of－GCMS（一）
BASIC THEORY AND USE OF GC-MSbyDr. Eugenia SobolevaContent1. Introduction.2. GC-MS systems and components.3. Vacuum system3.1. Rotary pump3.2. Di
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
赛默飞Ion－Torrent－Reporter软件获得CEIVD标志
赛默飞世尔科技近日表示，其 Ion Torrent Reporter 软件非整倍体 Dx 工作流程已获得 CE-IVD 标志。 CE-IVD 标志涵盖两个工作流程：低通全基因组非整倍体 Dx w1.0 和低通全基因组非整倍体 Mosaic Dx 1.0。这些工作流程将与一般的非体外诊断产品结合