HowtouseBasicLocalAlignmentSearchTool(BLAST)
DescriptionThe BLAST algorithm was developed as a way to perform DNA and protein sequence similarity searches by an algorithm that is faster than FASTA but considered to be equally as sensitive. Both of these methods follow a heuristic (tried-and-true) method that almost always works to find related sequences in a database search, but does not have the underlying guarantee of an optimal ......阅读全文
Online－Lectures－on－Bioinformatics
Biological preliminariesAnswersIntroductionReferencesExercisesAnalysis of individual sequencesIntroductionHelical wheels, amphipatic helices and coile
A－Guide－to－CORNET－for－the－Construction－of－Coexpression－and－ProteinProtein..
To enable easy access and interpretation of heterogenous and scattered data, we have developed a user-friendly tool for data mining and integratio
《科学》（20240517出版）一周论文导读
Science, Volume 384, Issue 6697, 17 May 2024《科学》，第384卷，6697期，2024年5月17日化学ChemistryDelocalized, asynchronous, closed-loop discovery of organic laser em
位点特异性核酸内切酶的蛋白质工程实验
实验材料 pMQ402试剂、试剂盒 阿拉伯糖苯甲基磺酰氟Ni-NTA 琼脂糖结合缓冲液清洗缓冲液洗脱缓冲液透析缓冲液仪器、耗材 超声破碎器冷冻离心机实验步骤 3. 方法此处列出的方法概括了为定点突变而做的氨基酸位点选择（见 3.1)、为在细菌细胞中表达而做的 MutH 蛋白突变 （ 见 3.2 )
位点特异性核酸内切酶的蛋白质工程实验
实验材料pMQ402试剂、试剂盒阿拉伯糖苯甲基磺酰氟Ni-NTA 琼脂糖结合缓冲液清洗缓冲液洗脱缓冲液透析缓冲液仪器、耗材超声破碎器冷冻离心机实验步骤3. 方法此处列出的方法概括了为定点突变而做的氨基酸位点选择（见 3.1)、为在细菌细胞中表达而做的 MutH 蛋白突变 （ 见 3.2 ) 和 Mu
Guidelines－for－the－Use－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal
What is Anesthesia? Anesthesia is a state of unconsciousness induced in an animal. The three components of anesthesia are analgesia (pain relief), amn
Basic－procedures－for－bacteria－culture2
E. Elution of DNA fragments from agaroseDNA fragments are eluted from low-melting temperature agarose gels using an unpublished procedure first develo
Basic－procedures－for－bacteria－culture1
A. Phenol extraction of DNA samplesPhenol extraction is a common technique used to purify a DNA sample (1). Typically, an equal volume of TE-saturated
DNA电泳
DNA电泳（主要内容如下） Preparation of Agarose Gel and Electrophoresis Extraction of DNA From Agarose Gel Extraction of DNA from Acrylamide Gels DNA Marker
The－UnderAgarose－Migration－Assay
overviewThe Under-Agarose assay is a useful method for observing the response of a cell population to one or more chemoattractant sources. The behavio
A－Method－for－Structure1
A Method for Structure–Activity Analysis of Quorum-Sensing Signaling Peptides from Naturally Transformable StreptococciMany species of streptococci se
Ingel－digestion－of－proteins－for－peptide－fingerprint－mapping
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension
peptide－fingerprint－mapping
Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples. Moreover, the development of two-dimension
Easy－Way－to－Clone－Genes－From－a－Phage－Library
Easy Way to Clone The protocol is oriented towards a C. albicans genomic library I made in Lambda Zap II on 7/97.The overall sequence of events is: •
How－to－make－DEPCtreated－water－and－Tris－Buffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at
How－to－perform－automated－counts－of－fluorescently－stained－cells.
NoteThis protocol describes semi-automated cell counts using fluorescently labeled cells, a hemocytometer and ImageJ software. The hemocytometer is n
How－do－I－decontaminate－my－tissue－culture－(Invitrogen)
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination. First, determine if the contami
New－white－paper－explains－how－to－increase－brewing－process－……
Ensuring production processes will operate as necessary and generate the desired product quality and yield in the required time is central to succ
氧气供应对处于浸浴状态的海马网络活动的重要性
过去对脑切片的研究， 对神经元和突触的基本特征方面已取得了丰富资料。但在完整的脑，进行中的的网络活动可能会严重影响这些特征。虽然，生理上更接近现实的网络活动模式已能在保存于界面型培养容器中的脑切片中成功地诱导出来，但此类资料却难以在浸浴型培养容器中取得，而浸浴型容器却能提供更好的实验优势，包
Belcher/Knight:－Electrocompetent－Cells
Electrocompetent CellsFrom Danijela Dukovski at Harvard Medical School. This protocol works well. --Julie NorvilleMaterialsDI water10% GlycerolSpecial
annealing－temp－for－degenerated－primer－－PCR－problem
PCR primers should be free of significant complementarily at their 3'-termini as this favours formation of primer-dimer which reduce product yield
筛分仪Sieve－Shaker－AS－450－basic简介
AS450 basic是强力振荡筛分仪AS450 control的基础版。 它用于干筛，筛分范围从 25 μm至125 mm，最大负载达15 kg。时间和振幅都是数字可调，可储存1组参数。对于需要大量样品干筛和湿筛的客户，AS450 basic是即经济又可靠的选择。应用实例化学制品, 土壤, 塑料,
General－Laboratory－Procedures,－Equipment－Use,－and－Safety－Considerations
A. Storage .The following properties of reagents and conditions are important considerations in processing and storing DNA and RNA. Heavy metals promo
Setup－and－use－of－a－twolaser－multiphoton－microscope
Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imagingDavid Entenberg,1 Jeffrey Wyckoff,1 Bojana Gligori
AvaSoftBasic标准版软件
AvaSoft-Basic标准版软件AvaSoft-Basic标准版软件是是随每套AvaSpec光谱仪系统免费赠送的标准软件包。AvaSoft-Basic软件界面友好，由鼠标指向控制下拉菜单。点击鼠标可以控制数据指针的移动，可以即时读取波长﹑像素值和 Y轴幅值。而按住鼠标左键或右键并拖曳可以方便并精
Use－of－the－Bradford－Protein－Assay－in－a－Microtiter－Plate－Format
Introduction The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change in
Guidelines－for－theUse－of－Analgesics－and－Tranquilizers－in－Laboratory－Animal2
GeneralSome of the drugs listed here do not possess all three criteria for an anesthetic and must be used in combinations to achieve full anesthesia o
5G/NR－－OTA－（四）
There is another reason why defining D gets difficult. It would get more difficult for UE case. In order to correctly define D, you need to hav
2011溶出技术国学术研讨会
药物新剂型的迅速发展，对药物溶出技术带来诸多挑战，成为业界研究的热门专题，它不但对中西制药的检测起著重要作用，亦是评价药物质量的关键性指标之一。 力扬企业有限公司(Nikyang)及瑞士 Sotax 公司一直致力于服务制药业及相关行业，在制药工业检验仪器的开发制造中始终处于世界领先地位
RNAi相关术语
1.RNAi ：（RNA interference）RNA干扰一些小的双链RNA可以高效、特异的阻断体内特定基因表达，促使mRNA降解，诱使细胞表现出特定基因缺失的表型，称为RNA干扰（RNA interference，RNAi ，也译作RNA干预或者干涉）。它也是体内抵御外在感染的一种重要保护机制