AuxinImmunolocalizationinPlantTissues
The major naturally occurring auxin, indol-3 acetic acid (IAA), coordinates many growth and differentiation processes by modulating gene expression during plant development. The sites of IAA biosynthesis and its polar transport (PAT) routes determine auxin accumulation and distribution during growth. From many studies on the model plant Arabidopsis thaliana over the last years, it has become evident that th......阅读全文
Auxin－Immunolocalization－in－Plant－Tissues
The major naturally occurring auxin, indol-3 acetic acid (IAA), coordinates many growth and differentiation processes by modulating gene expressio
Protein－Immunolocalization－in－Maize－Tissues
The analysis of gene expression at transcript and at protein level is of outstanding importance in plant developmental biology. Proteins can be loc
Rapid－DNA－Isolation－from－Phyllanthus－Amarus－and－Other－Plant－Tissues
Procedure Preheat Extraction Buffer at 60°C. Weigh 100 mg of fresh leaf tissue and grind it to powder in Liquid Nitrogen in a chilled mortar an
Quantifying－Kinetics－of－Net－Ion－Fluxes－from－Plant－Tissues－by－Noninvasi...
Non-invasive microelectrode ion flux measuring (the MIFE system) allows concurrent quantification of net fluxes of several ions with high spatial
Extraction－of－RNA－from－Fibrous－tissues
实验概要E.Z.N.A.™ MicroElute® Total RNA Kit provides a rapid and easy method for the isolation of up to 50 ug of total RNA from small amount of cultured
Protein－extraction－from－whole－tissues－for－IEF
Modified from that of Jay Thelen - University of Missouri-ColumbiaPhenol extraction followed by methanolic ammonium acetate precipitation - an effecti
FIXATION－OF－PLANT－METAPHASE－CHROMOSOMES
Reagents (a) Metaphase arresting agents: Choose one of the following (Note 2) and shake vigorously to aerate before putting in living plant materi
PCR－from－Plant－Tissue
1.protocol(1)collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH(2)put in boiling H2Ofor 30 sec
A－quick－method－to－isolate－plant
Use from 0.01 - 0.1 gram plant material. Grind the plant material with liq.N2 in a mortar.We normally use some alumina to crush hard tissue.
ChIP－using－plant－samples
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin immunoprecipitati
PCR－from－Plant－Tissue
PCR from Tissue Reference: Klimyuk et.al., 1993, Plant J. 3:493-494 Last updated: 1/27/00 By: Kay Schneitz collect piece of tissue (e.g., piec
Plant－Genome－Annotation－Methods
Annotation of plant genomic sequences can be separated into structural and functional annotation. Structural annotation is the foundation of all g
李家洋应邀在PLANT－CELL撰写Reflections－on－Plant－Cell－Classics文章
The Plant Cell是植物领域的著名学术期刊，对植物学的发展起到了重要的引领作用。为庆祝创刊30周年，The Plant Cell杂志社邀请部分编委会成员及其他科学家对发表在该杂志的重要研究工作进行评述，以期增进对植物科学过去30年中重要研究进展的认识，激发对未知领域的探索。 李家洋研
微生物所揭示miRNA调控植物生长素信号途径的机制
microRNA（miRNA）是一类广泛存在于生物体的21nt到24nt的短的非编码RNA，通过碱基互补配对的方式介导其靶标mRNA的剪切或者抑制其翻译。在植物中，miRNA主要通过剪切靶标mRNA调控生长发育以及抗病抗逆作用。植物生长素（auxin）信号途径在植物生长发育过程中具有重要的调控作
Approaches－to－Identifying－Genes－for－Salinity－Tolerance－and－the－Importance..
Soil salinity reduces the ability of plants to take up water, and this quickly causes reductions in the rate of cell expansion in growing tissues.
MS－Plant－Tissue－Culture－Medium
Component mg/l in MS mg/l in stock Amount for
ChIP－using－plant－samples－–－Arabidopsis
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
Chemical－Genomics－Approaches－in－Plant－Biology
Chemical genomics (i.e., genomics-scale chemical genetics) approaches are based on the ability of low-molecular-mass molecules to modify biologica
Manipulating－Expression－of－Tonoplast－Transporters
Plant vacuoles have multifaceted roles including turgor maintenance, cytosolic pH and ionic homeostasis, plant protection against environmental st
我国揭示ABI1sHSP22在激素ABA和Auxin互作中的作用机制
植物激素脱落酸(ABA)和生长素(auxin)相互协同或拮抗调控了植物生长发育和应对环境胁迫的过程。深入解析两种激素的交叉作用机制对于农业生产中定向编辑基因、培育优良性状具有重要意义。目前已鉴定多个既能响应脱落酸也能响应生长素的基因，但其交叉作用机制理解甚少。图: ABI1-sHSP22介导AB
E.Z.N.A.®－Total－RNA－Maxi－Kit－Protocol－for－Animal－Tissues
实验概要The E.Z.N.A.® Total RNA Maxi Kit uses HiBind® matrix spin-column technology to isolate up to 5 mg total cellular RNA from a variety of sources
植物病毒(plant－viruses)RNA提取
大多植物病毒RNA为单链RNA，并且其极性与mRNA极性相同，植物病毒RNA提取较为简单，一般使用酚氯仿即可获得满意结果。 一、材料 提纯TMV病毒液（10mg/ml）。 二、设备 冷冻台式离心机，低温真空干燥仪，电泳仪，电泳槽。 三、试剂 TE
Production－of－Recombinant－Proteins－in－SuspensionCultured－Plant－Cells
Plants have emerged in the past decade as a suitable alternative to the current production systems for recombinant pharmaceutical proteins and, toda
版纳植物园拟南芥WRKY57转录因子研究获进展
植物叶片衰老受到多种发育因子和环境因子所调控。外源植物激素茉莉酸(JA)处理可以诱导叶片细胞迅速进入衰老程序，而生长素(Auxin)却可以有效地抑制该过程发生。众所周知，植物激素JA和auixn介导的信号途径之间存在着交叉调控通路，并在植物发育和抵抗病原菌侵染等生理过程中发挥着重要调控功能。但是
版纳植物园揭示OsIAA4参与生长素介导的水稻株型建成
水稻是我国最重要的粮食作物之一，我国人口在未来20年仍将继续增长，对粮食的需求将持续增加，但耕地面积却在不断减少，因此提高主要农作物单产是实现粮食总产量增长的根本途径。按照作物产量性状遗传改良的实践，通过改良株型，提高品种的田间种植密度，进而促进光能利用率，可以增加作物产量。株型发育是当前及未来
E.Z.N.A.®－Total－RNA－Midi－Kit－Protocol－for－Eukaryotic－Cells－and－Tissues
实验概要E.Z.N.A.® Total RNA Midiprep Kit provides a rapid and easy method for the isolation of up to 600 ug of total RNA from cultured eukaryotic cells,
RNA提取
RNA提取（主要内容如下）Tips for Handing RNA Total RNA IsolationmRNA IsolationrRNA IsolationOthersQ & A posted in the Method Forum Basic Procedures for Handing
Characterization－and－Applications－of－PlantDerived－Recombinant－Antibodies
Expression of foreign proteins in plants has become a standard technique in plant molecular biology. Various plant species have been used to produ
Synthesis－of－Recombinant－Human－Cytokine－GMCSF－in－the－－Seeds－of－Transge...
We are interested in studying plant systems as vehicles for the production of recombinant proteins of clinical relevance. There are a number of po
Isolation－of－Cell－Wall－Proteins－from－Medicago－sativa－Stems
Plant cell walls are highly dynamic and chemically active components of plant cells. Cell walls consist primarily of polysaccharides, with protein