PlantGenomeAnnotationMethods
Annotation of plant genomic sequences can be separated into structural and functional annotation. Structural annotation is the foundation of all genomics as without accurate gene models understanding gene function or evolution of genes across taxa can be impeded. Structural annotation is dependent on sensitive, specific computational programs and deep experimental evidence to identify gene features within g......阅读全文
Plant－Genome－Annotation－Methods
Annotation of plant genomic sequences can be separated into structural and functional annotation. Structural annotation is the foundation of all g
植物基因组DNA提取试剂盒MagExtractor®Plant－Genome
产品索引 5871 中文名称： 植物基因组DNA提取试剂盒 英文名称： MagExtractor®-Plant Genome- 产品编号： NPK -500 产品类别： 分子生物学 生产厂家： TOYOBO 产品价格：
Gene－Structure－Annotation－at－PlantGDB
The accurate identification of exons and introns that comprise a complete plant gene structure can be a time-consuming and challenging task. Novel
sothing－about－Genome－walking
Can anyone recommend a good kit for genome walking? I would like to find out the promoter sequence of a known gene. Is genome walking the way to do it
Auxin－Immunolocalization－in－Plant－Tissues
The major naturally occurring auxin, indol-3 acetic acid (IAA), coordinates many growth and differentiation processes by modulating gene expressio
PCR－from－Plant－Tissue
PCR from Tissue Reference: Klimyuk et.al., 1993, Plant J. 3:493-494 Last updated: 1/27/00 By: Kay Schneitz collect piece of tissue (e.g., piec
FIXATION－OF－PLANT－METAPHASE－CHROMOSOMES
Reagents (a) Metaphase arresting agents: Choose one of the following (Note 2) and shake vigorously to aerate before putting in living plant materi
ChIP－using－plant－samples
实验概要 The immunoprecipitation (IP) of cross-linked chromatin with antibodies specific for certain histone modifications (chromatin immunoprecipitati
A－quick－method－to－isolate－plant
Use from 0.01 - 0.1 gram plant material. Grind the plant material with liq.N2 in a mortar.We normally use some alumina to crush hard tissue.
PCR－from－Plant－Tissue
1.protocol(1)collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH(2)put in boiling H2Ofor 30 sec
李家洋应邀在PLANT－CELL撰写Reflections－on－Plant－Cell－Classics文章
The Plant Cell是植物领域的著名学术期刊，对植物学的发展起到了重要的引领作用。为庆祝创刊30周年，The Plant Cell杂志社邀请部分编委会成员及其他科学家对发表在该杂志的重要研究工作进行评述，以期增进对植物科学过去30年中重要研究进展的认识，激发对未知领域的探索。 李家洋研
全基因组的比较基因组杂交技术介绍
Whole-Genome and Custom Fine-Tiling Array CGHComparative Genomic Hybridization (CGH) measures DNA copy number differences between a reference genome a
Chemical－Genomics－Approaches－in－Plant－Biology
Chemical genomics (i.e., genomics-scale chemical genetics) approaches are based on the ability of low-molecular-mass molecules to modify biologica
MS－Plant－Tissue－Culture－Medium
Component mg/l in MS mg/l in stock Amount for
ChIP－using－plant－samples－–－Arabidopsis
实验概要This protocol describes how chromatin is prepared from Arabidopsis, which can subsequently be used for chromatin immunoprecipitation (ChIP). T
生物信息学教程
Sequence - Evolution - FunctionComputational Approaches in Comparative GenomicsbyEugene V. KooninNational Center for Biotechnology Information, Bethes
A－Collection－of－PlantSpecific－Genomic－Data－and－Resources－at－NCBI
The National Center for Biotechnology Information (NCBI) provides a data-rich environment in support of genomic research by collecting the biologi
Production－of－Recombinant－Proteins－in－SuspensionCultured－Plant－Cells
Plants have emerged in the past decade as a suitable alternative to the current production systems for recombinant pharmaceutical proteins and, toda
植物病毒(plant－viruses)RNA提取
大多植物病毒RNA为单链RNA，并且其极性与mRNA极性相同，植物病毒RNA提取较为简单，一般使用酚氯仿即可获得满意结果。 一、材料 提纯TMV病毒液（10mg/ml）。 二、设备 冷冻台式离心机，低温真空干燥仪，电泳仪，电泳槽。 三、试剂 TE
软件：Genomewide－Association－Study－Software
The software included are listed bellow IMPUTEa program for genotype imputation in genome-wide association studies and fine-mapping studies based on a
Genome－Biology：为CRISPR选择最佳向导
细菌一直在与病毒或入侵核酸进行斗争，为此它们演化出了多种防御机制，CRISPR-Cas9 适应性免疫系统就是其中之一。规律成簇的间隔短回文重复CRISPR与内切酶Cas9的组合，可以在引导RNA的指引下，靶标并切割入侵者的遗传物质。 2012年研究者们利用这一特点，将CRISPR系统发展成了强大
Genome－Biology发文介绍RNA印记法
生物学家们逐渐意识到，RNA不只是DNA和蛋白之间的过渡产物，它对调节基因的表达有重要作用。深入研究RNA调控，能够帮助科学家们进一步理解相关疾病。 宾夕法尼亚大学的科学家们开发了分析RNA调控的新方法，这一方法能够有效获得RNA与RNA结合蛋白(RBP)互作的所有位点。这一发表在Geno
Genome－Biology：食物会影响DNA序列
牛津大学的研究人员在两种寄生虫中检测到了食物组分造成的DNA序列差异。他们在Genome Biology杂志上发表文章指出，食物能够影响生物的基因序列。 “生物从食物中获取原料构建自己的DNA。我们认为，食物组分可能应该能够改变生物的DNA。我们也许可以预测素食者熊猫与肉食者北极熊之间的基因差
A－Guide－to－CORNET－for－the－Construction－of－Coexpression－and－ProteinProtein..
To enable easy access and interpretation of heterogenous and scattered data, we have developed a user-friendly tool for data mining and integratio
Characterization－and－Applications－of－PlantDerived－Recombinant－Antibodies
Expression of foreign proteins in plants has become a standard technique in plant molecular biology. Various plant species have been used to produ
Genome－Res展现肿瘤与免疫的互动
现在，癌症的免疫疗法越来越受到人们的重视。去年，Science杂志将癌症免疫疗法被评为了“年度突破”之一。不过开发这类抗癌药物并不容易，因为肿瘤的突变模式存在很高的异质性，人体的免疫系统也各不相同。 在一项新研究中，研究人员分析了大量的肿瘤样本，并在此基础上鉴定了可以激活患者免疫系统的肿瘤突变
Genome－Research：常见病源自基因调控
许多罕见疾病都是由基因突变引起的，例如镰状细胞贫血。然而，人们至今还未能确定引起复杂常见病(类风湿关节炎等)的基因突变。 Case Western Reserve大学医学院的研究人员对六种复杂的常见病进行研究，向人们展示这些疾病的病因在于基因以外的DNA改变，文章发表在Genome
南京农大长江学者Plant－Cell重要成果
近期，国际植物科学领域顶级期刊《Plant Cell》刊登了南京农业大学和美国俄勒冈州立大学关于植物保护生物学的最新研究成果。这项研究表明，PsXEG1有助于大豆疫霉菌的毒力，但大豆可识别PsXEG1以诱导免疫反应，这反过来又被RXLR效应物所抑制。因此，XEG1代表一个质外体效应蛋白，可通过植
Extraction－of－DNA－From－Plants－Using－Plant－DNAzol®－Reagent
实验概要Plant DNAzol® is an extra-strength-DNAzol® reagent (patent pending) specifically formulated for the isolation of genomic DNA from plants. The Plan
植物叶蛋白（the－plant－leaf－protein）的提取
一、实验目的熟悉植物叶蛋白的几种提取原理和方法，了解其意义及其应用价值。二、实验原理植物叶蛋白或称绿色蛋白浓缩物 (leaf protein concentration，简称LPC)，是从新鲜植物叶片中提取的高质量浓缩蛋白质，不仅是畜禽生长发育和生产畜产品的主要营养物质，而且目前也正成为人类的保