GeneralLaboratoryMethodsforTetrapyrroles
There are thousands of porphyrins and metalloporphyrins, and hundreds of new derivatives appear each year. This variety arises because the cyclic conjugated tetrapyrrole nucleus (Figure 1 ) can have different substituents at the eight β-pyrrole positions, at the four meso [5,10,15,20] carbon atoms, and N-alkyl groups can be added to the four central nitrogen atoms. Since its synthesis in 1972, over 8000......阅读全文
Basic-Methods-of-Culturing-Drosophila
实验概要Basic Methods of Culturing Drosophila实验步骤Stockkeeping1. Mechanics Most stocks can be successfully cultured by periodic mass transfer of a
Methods-for-Xylem-Sap-Collection
Xylem and phloem are essential for the exchange of solutes and signals among organs of land plants. The synergy of both enables the transport and
Staining-Methods-for-cell-death
The simplest way: trypan blue. Dead cells stain blueNon-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead
Milestone微波化学平台新品发布会邀请函
尊敬的女士/先生:您好! 意大利Milestone公司2014微波化学平台新产品发布会将于9月25日召开,Milestone公司总经理Diego先生和中国区总代北京莱伯泰科公司,诚意邀请您莅临参加,共同体验分享Milestone新品带给您全新的惊喜! Milestone公司生产微波
AB-SCIEX-和ARUP实验室联合开发激素分析临床研究新方法
2011年2月7日——全球生命科学分析技术的领导者AB SCIEX 公司,今天宣布与美国主要的临床基准实验室ARUP 实验室合作,共同开发新的测试方法供全球实验室采用,从而提高对激素的分析水平,在使用了AB SCIEX的QTRAP® 5500质谱技术后,ARUP的研究人员得到了以前临床
The-TRC-shRNA-Design-Methods-and-Rules
OverviewWe design shRNA molecules with an algorithm. Our algorithm uses several criteria to rank potential 21mer targets within each human and mouse R
The-Fungal-Genetics-Stock-Center-Methods
Some basic Neurospora methods compiled at StanfordVogel's Minimal MediumD. D. Perkins - Hints for the care, feeding and breeding of NeurosporaStra
COMPARISION-OF-DIFFERENT-PROTEIN-DETERMINATION-METHODS
COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODSCompanyMethodDetection RangeApplications -CompatibilityAssay protocolPrecautions-InterferencesA
Tissue-Culture-Methods3
REFERENCES:R. Ian Freshney, Culture of Animal cells: A manual of basic techniques, Wiley-Liss, 1987.VI. TISSUE CULTURE PROCEDURESEach student should m
Tissue-Culture-Methods1
I. TYPES OF CELLS GROWN IN CULTURETissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often us
Tissue-Culture-Methods2
IV. MAINTENANCECultures should be examined daily, observing the morphology, the color of the medium and the density of the cells. A tissue culture log
《Nature-Methods》大脑地图图解
多年来,出现了多种“大脑地图”,每一种都关注不同的大脑过程,从新陈代谢到认知功能。虽然这些地图很重要,但单独使用它们会限制研究人员从中得出的发现。 现在,来自蒙特利尔神经学研究所的一个团队和其他研究人员在一个地方汇集了40多张现有的大脑地图。这个被称为神经图谱的数据库有望帮助科学家发现不同大脑
Guide-to-Cell-Proliferation-and-Apoptosis-Methods
Chapter 1: Cell Death - Apoptosis and Necrosis1.1Introduction21.1.1Terminology of cell death21.1.2Differences between necrosis and apoptosis31.1.3Apop
国际纯水标准
International Organization for Standardization specification for water for laboratory use ISO 3696: 1995 This standard covers three grades of water as
Human-Embryonic-Stem-(ES)-Cell-Protocols——General-notes-on-ES-cell-culture
hES media has a two week shelf life.hES cells should be cultured in 4, 6, 24, 48, 96 well plates. Growing cells in flasks is not recommended because i
Capture-ELISA
实验概要Capture ELISA (also known as "sandwich" ELISA) is a sensitive assay to quantitate picogram to microgram quantities of substances (such as hormon
Guest-Moderator:-Guangtao-Yan
Guangtao Yan Guangtao Yan, Researcher, professor and doctoral supervisor. He is currently the deputy director of Center for Clinical Laboratory Medic
The-Impact-of-Harmo...-(一)
实验概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and reliable a
Cryopreservation-of-Cell-Lines
AimThe protocol below describes the use of passive methods involving an electric -80ºC freezer for the cryopreservation of cell cultures. ECACC routin
BCEIA2019分析检测与体外诊断国际高峰论坛日程安排(Eng)
International Summit on Analytical Instrumentation and In Vitro Diagnosis Meeting Agenda Date:2019.10.23 09:30-12:45Location:InterContinental Beijing
Nature-Methods:预测干细胞的命运
多伦多大学的研究人员开发出了一种方法,可以快速地筛选人类干细胞以及更好地控制它们的转化。这一技术有潜力应用于再生医学和药物研发。研究结果发表在本周的《自然方法》(Nature Methods)杂志上。 这项研究工作是由多伦多大学加拿大生物工程学首席科学家Peter Zandstra
Nature-Methods发布新RNA测序技术
Santa Cruz公司和Rochester大学的研究人员开发了一种新的RNA测序技术。他们通过这一技术发现了许多此前未被检测到的调控性小RNA。这一成果发表在八月三日的Nature Methods杂志上。 这个新技术可以在细胞中灵敏检测到带有化学修饰(甲基化)的小RNA。“tRNA是生物体内
Methods-for-the-Measurement-of-a-Bacterial-Enzyme-Activity-in-Cell-Lysates
AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts of Helicobacte
PREPARATION-OF-2%-FORMALDEHYDE-STOCK-SOLUTION-(2-METHODS)
METHOD 1:Formaldehyde preservative – 2% formaldehyde solution in protein-free phosphate-buffered saline (PBS).Prepare as follows:Add 2 g paraformaldeh
Nature-Methods揭示AIDS的惊人动态
Emory大学的科学家们利用一种新成像技术,首次在活体动物(恒河猴)中获得了全身性的艾滋病毒复制实时图谱,揭示了猴艾滋病毒SIV出人意料的藏身处。这项发表在Nature Methods杂志上的研究显示,当猴子接受抗逆转录病毒(ARV)药物治疗时,机体不同位置残留的病毒量并不相同。研究人员开发的新
Nature-Methods新年展望:高多重成像
今年第一期《Nature Methods》评出了2015的年度技术——单颗粒冷冻电镜(cryo-EM)。除此之外,该杂志还对一些热门技术进行了一番展望,包括细胞内蛋白标记、精准光遗传学、高多重成像、亚细胞图谱分析等等。 荧光团之间的光谱重叠,是成像复杂生物学结构的一个主要障碍。这种限制让绝大多
The-TRC-shRNA-Design-Methods-and-Rules2
Score the Candidates Sequences for SpecificityWe are forced to balance the prediction of knockdown efficiency against the desire to minimize interacti
上海精卫中心林萍团队阐明精神分裂症候选生物标志物
精神卫生是全球关注的热点,已成为影响人类健康的重大公共卫生事件。精神与心理疾病病因复杂且多因素。《General Psychiatry》杂志2022年35卷第1期发表了上海交通大学医学院附属精神卫生中心陈京红、郭晓奎、程训佳、林萍、娄晓燕等检验科团队的《The national-wide con
Cellular--Molecular-Pathology-Branch
VisionTo provide scientific collaboration of excellence to National Toxicology Program (NTP) (http://ntp.niehs.nih.gov/) interdisciplinary research pr
免疫细胞化学
Introduction to Immunocytochemistry (House Ear Institute)A brief overview of common available methods. BrDU Immunocytochemistry using peroxidase and