InVitroProductionofSweetPeas(LathyrusodoratusL.)viaAxillaryShoot
The genus Lathyrus is best known because it includes a number of wild relatives of the protein pea which, despite being generally neglected and under-utilised, hold considerable potential as a useful genetic resource for the acquisition of interesting stress resistant traits important for a sustainable agriculture. However, also included in this genus are important commercially produced species with a sig......阅读全文
In－Vitro－Production－of－Sweet－Peas－(Lathyrus－odoratus－L.)－via－Axillary－Shoot
The genus Lathyrus is best known because it includes a number of wild relatives of the protein pea which, despite being generally neglected and un
如何应对植物组培过程中的污染问题？
植物组织培养是20世纪初发展起来的一门新兴技术，随着这项技术的日益完善，其应用也越来越广泛。但是在组织培养过程中，常常会遇到污染问题使实验无法进行下去，甚至导致整个实验失败，给科研和工厂化生产带来损失。污染是指在植物组织培养过程中，培养基和培养材料滋生杂菌，最终导致培养失败的现象。常见的污染类型包括
In－Vitro－Conservation－and－Cryopreservation－of－Ornamental－Plants
Today, the conservation of ornamental germplasm can take advantage of innovative techniques which allow preservation in vitro (slow growth storage
Retrovirus－Production
Material:Packaging Cells, e.g. Phoenix cells (an adenovirus Ad5-transformed human embryonic kidney cell line 293T, transfected with two MoMLV packagin
L.－acidophilus－transformation
OverviewElectrotransformation procedure for Lactobacillus acidophilusProcedurePrepare Electrocompetent cellsInoculate overnight culture at 10^6 CFU/ml
Polyclonal－Antibody－Production
实验概要Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation.实验步骤Rabbit Immunization Initial
Switchgrass－(Panicum－virgatum－L.)
During the last decade, Agrobacterium -mediated transformation of more than a dozen monocotyledonous plants, including forage and turf grasses, ha
In－Vitro－Fertilization
When we first started using X. tropicalis, in vitro fertilization had an extremely poor efficiency. However, with the careful selection of a mature ma
Production－of－Antibody－Fragments－in－Arabidopsis－Seeds
Plants offer a number of attractive benefits over conventional mammalian or bacterial cell culture systems for the production of valuable pharmace
In－vitro－Sphingomyelinase－Assay
Reagents:Lysis buffer25 mM Tris-HCl, pH 7.45 mM EDTA1 mM ATP20 µg/ml CLAP1 mM PMSFBuffer A10 mM MgCl20.2 M Tris-HCl, pH 7.40.2 % Triton X-100Buffer B0
In－vitro－growth－of－seedlings
sterilisation of seeds: rinse with 70% EtOH for 30 sec put in 1% bleach (sodium hypochlorite, supplemented by a few drops of Tween-20) for 5 mi
常见生物染色体数目列表
常见生物染色体数目列表通名学名双倍体数动物人类Homo sapiens46弥猴Macacamalatta42黄牛Bostaurus60猪Susscrofa38狗Canis familiaris78猫Felis domesticus38马Equus Calibus64驴Equus asinus62山羊
常见生物染色体数目列表
常见生物染色体数目列表通名学名双倍体数动物人类Homo sapiens46弥猴Macacamalatta42黄牛Bostaurus60猪Susscrofa38狗Canis familiaris78猫Felis domesticus38马Equus Calibus64驴Equus asinus62山羊
Production－of－Recombinant－Proteins－in－SuspensionCultured－Plant－Cells
Plants have emerged in the past decade as a suitable alternative to the current production systems for recombinant pharmaceutical proteins and, toda
Production－of－neuronpreferential－lentiviral－vectors
实验概要Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells and are toxic to infected cells.
Gene－Expression－Analysis－of－Shoot－Apical－Meristem－Cell－Types
Shoot apical meristems (SAMs) of higher plants harbor a set of stem-cells and provide cells for the development of all the above-ground biomass of
In－vitro－culture－of－embryonic－lungs
In vitro culture of embryonic lungsfrom Hogan LabIsolation of Lung Bud EndodermWhat you need:E11-12 mouse embryosDMEM with 5% fetal bovine serumpetri
In－Vitro－Protein－Ubiquitination－Assay
Ubiquitination is one of the most important posttranslational modifications in all eukaryote organisms. Ubiquitin-activating enzyme (E1), ubiquiti
InVitro－Adipocytes－Differentiation
IntroductionObesity is a significant clinical problem that contributes to life-threatening diseases such as diabetes and atherosclerosis. With an incr
体外重组（in－vitro－recombination）
载体与外源DNA分子体外重组时，如何选择优化连接条件以达到最高的重组率。因此有必要根据影响连接效率的因素综合考虑连接条件。影响连接效率的因素很多，如反应温度、插入片段和载体之间的摩尔比、DNA末端性质、反应时间、ATP浓度等。1. 反应温度是比较重要的影响因素。因为连接酶的最适反应温度为37℃，
In－Vitro－T－Cell－Activation
In Vitro T Cell ActivationIntroductionMature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR).
Coating－of－Platelets－with－Antibody－in－vitro
OUTLINEAntibody-coated platelets (opsnized) may be used in the subsequent thrombophagocytosis assay. PROTOCOLResuspend 1.6x10^8 of CMFDA-labeled plate
The－Role－of－Eosinophils－in－the－Chemokine－Network－of－Allergy
The inflammatory response associated with asthma is characterized by the recruitment of eosinophils from the bronchial microcirculation in response to
Th17－Polarization－of－Mouse－CD4－Cells
实验概要T helper 17 (Th17) cells are a subset of CD4 T helper cells characterized by their production of IL-17, particularly IL-17A and IL-17F. They are
Invitro－Phagocytosis－Assay－of－Macrophages
IntroductionThe term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular pr
器官培养
In vitro organ cultures (Nagy Lab)kidneylungslimb In Vitro Differentiation of ES Cells into: (Nagy Lab)Cardiac MuscleNeuronal LineagesCystic Embryoid
SpectraPen/PolyPen手持式光谱仪应用案例——光质培养研究
植物生长过程中，光照是必不可少的。一方面光照是植物光合作用的能量来源；另一方面光照能够调节植物生长的一系列生理活动。因此，使用不同光强和不同光质（颜色或波长）的光源对植物进行培养实验，一直是研究植物生长调节的重要课题。 这类研究中，既要配备不同光质的光源对植物进行培养，同时还要测量光源的光强和光质（
Ultraviolet－irradiation－impairs－epiboly－via－microtubules－in－Zebrafish
IntroductionZebrafish have transparentembryos that develop outside the mother. They develop rapidly, so that at 24 hours after fertilization, the embr
Targeted－Gene－Replacement－in－Fungal－Pathogens－via－Agrobacterium－...
Genome sequence data on fungal pathogens provide the opportunity to carry out a reverse genetics approach to uncover gene function. Efficient meth
BTC100微根窗根系观测技术参考文献
美国BARTZ公司最早研制生产微根窗根系观测仪器，BTC-100成为国际上应用最广、发表论文最多的根系研究仪器技术，其学术研究论文涵盖几十种世界知名学术期刊和专著，包括Science、Nature、Global change biology（影响因子8.502）、New Phytologist（影响