Follicularthyroidcellisolationandculture
Thyroid epithelial cells (also called follicular cells or principal cells) are cells of thyroid gland that are responsible for the production and secretion of thyroid hormones, that is, thyroxine (T4) and triiodothyronine (T3). The thyroid epithelial cells take up iodine and amino acids from the blood circulation on the basolateral side, synthesize thyroglobulin and thyroperoxidase from amino acids an......阅读全文
Isolation－of－dermal－mast－cell－(DMC)
1. Human DMC were isolated from skin by enzymatic digestion.2. Dermal tissue was obtained from patients with skin after informed consent was given.3.
细胞分离技术（Cell－Isolation）
一、离心技术离心是研究如细胞核、线粒体、高尔基体、溶酶体和微体，以及各种大分子基本手段。一般认为，转速为10~25Kr/min的离心机称为高速离心机；转速超过25Kr/min，离心力大于89Kｇ者称为超速离心机。目前超速离心机的最高转速可达100Kr/min，离心力超过500Kg。（一）、差速离心（
Isolation－and－culture－of－SpragueDawley－rat－aortic－smooth－muscle－cells
The intact mature arterial media is composed of at least four phenotypically unique cell subpopulations that reside in distinct medial layers. T
Isolation－of－cell－nuclei－for－the－application－in－the－cellfree－system
Characteristics of the procedurePreparation of isolated nuclei - procedurePreparation of radioactive labeled nucleiMaterial Characteristics of the pro
ES－Cell－Culture－and－Manipulation3
Care and Handling of Feeder Layer Cells STO/SNL cells are a derivative of the standard STO cell line. They are transformed with a LIF producing pl
The－Dos－and－Donts－of－Cell－Culture
Given below are a few of the essential "do’s and don'ts" of cell culture. Some of these are mandatory e.g. use of personal protective equipment (P
A－primary－cell－culture－model－of－rabbit－uroepithelium
Isolation of Epithelial Cells from Rabbit Bladders 1. Animal experiments were performed in accordance with the Animal Use and Care Committee. 2. Urina
PC12－Cell－Culture－and－Fusion
Cell CultureMaterials1. Falcon Primaria culture dishes.2. Culture medium: DME (or F12K) with glutamine, supplemented with 7% heat-deactivated horse se
Aseptic－Technique－and－Good－Cell－Culture－Practice
AimTo ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross con
ES－Cell－Culture－and－Manipulation2
Picking ES cell clonesOne or two days before picking colonies prepare 24-well plates of feeders. You can also use alternate protocols that utilize 96-
Isolation－of－retinal－pigment－epithelial－cell（二）
15. This medium is replaced after 1 day with 5% serum-containing RPE medium, and subsequent changes were made every 2 to 3 days.16. After 3 to 4 weeks
Mouse－B－cell－isolation－with－magnetic－beads
实验概要Isolate B cells from total mouse cells实验步骤 Praparation:1. Enough MACS; put RPMI into water bath.2. Put some MACS in 15 ml tube or 1.5
Isolation－of－retinal－pigment－epithelial－cell（一）
The pigmented layer of retina or retinal pigment epithelium (RPE) is the pigmented cell layer just outside the neurosensory retina that nourishes
MEDIA－AND－SOLUTIONS－REQUIRED－FOR－ROUTINE－ES－CELL－CULTURE
Media UsedTo prepare 100 ml mediumDMEM80 mlFCS15 mlNon-essential amino acids (100x)1 mlPen/strep (5,000 1U/ml, 5000 ug/ml)1 mlL-Glutamine 200 mM1 mlNu
293细胞培养(cell－culture)技术
1、293细胞明显适应酸性环境,pH值在6.9～7.1时,可顺利贴壁生长, 换液时动作要轻。一般用高糖的DMEM培养基。2、传代：倒去废液，PBS洗一次（轻），用0.02%EDTA与0.25%Trypsin消化，生长良好细胞，培养瓶中轻摇,使之流遍所有细胞表面,即将其吸除或弃去消化30s，然后吸去，
胚胎干细胞培养
Media and Solution required for ES Cell Culture (Bowtell Lab) Routine Culturing of ES Cells (Bowtell Lab) Routine Splitting and freezing of cells (
Human－Embryonic－Stem－(ES)－Cell－Protocols——General－notes－on－ES－cell－culture
hES media has a two week shelf life. hES cells should be cultured in 4, 6, 24, 48, 96 well plates. Growing cells in flasks is not recommended be
Isolation－of－Cell－Wall－Proteins－from－Medicago－sativa－Stems
Plant cell walls are highly dynamic and chemically active components of plant cells. Cell walls consist primarily of polysaccharides, with protei
胚胎干细胞培养技术大全
MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE Routine Culturing of ES Cells ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS MITOM
植物细胞培养（plant－cell－culture）技术概述
植物细胞培养技术就是为了某种目的而在细胞水平上对离体植物细胞或原生质体进行的一系列生物工艺学操作。它包括分离、培养、再生以及一系列相关的操作。就有用化合物的生产来说，它主要是指在无菌条件下通过悬浮培养植物细胞生产有用化合物的过程。理论与技术基础：植物细胞全能性、微生物液体深层发酵系统、遗传工程
Isolation－of－papillary－cells
实验概要 This protocols provides a general protocol for isolation of papillary cells. 实验步骤 Isolation of renal papillary cells 1. For isolation of pa
Mitochondrial－DNA－Isolation－from－Somatic－Embryogenic－Cell－Cultures－of－Larix
Mitochondrial DNA is isolated by a modification of the methods described by Wilson and Chourey (1984) and Radetzky (1990). Cell cultures at four d
Culture－of－retinal－endothelial－cells－and－pericytes
Isolation of retinal microvessels1. Eyes were obtained and transported on ice.2. Eyes were cut circumferentially 3 mm posterior to the limbus, the v
Isolation－of－papillary－cells
Isolation of renal papillary cells1. For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/
Stem－cell－characteristics－of－amniotic－epithelial－(AE)－cells
Isolation of AE Cells1. Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesarean
原代神经细胞培养方法－Neuron－Cell－Culture
1. Preparation of coverslips1.1- Mass cultureOur standard mass cultures are plated on astrocytes. Those, in turn, are plated on glass coverslips pre-
细胞组织消化常用的几种酶的选择
直接从生物体获取的组织，一般需要将其消化成单个细胞才能进行体外培养。这种直接从离体组织获得的细胞，更接近于生物体内的生活状态，且生物性状尚未发生很大改变，因此在药物筛选、细胞移植、类器官培养、肿瘤研究等众多领域备受欢迎。但组织消化过程中常遇到多种问题，例如消化不完全、细胞死亡率高等。如何克服这些问题
Primary－cardiac－fibroblast－and－cardiomyocyte－isolation
1. Ventricles were removed under sterile conditions. 2. Ventricles were placed in cold sterile primary cell culture medium, minced into approximat
Isolation－of－stromal－vascular－cells－from－human－adipose－tissue
Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal stem cells in tissues and the la
Isolation－and－Transcription－Profiling－of－Purified－Uncultured－Human－Strom...
Isolation and Transcription Profiling of Purified Uncultured Human Stromal Stem Cells Isolation and Transcription Profiling of Purified Uncult