Isolationandcultureofhumanpulmonaryarterysmoothmusclecells...
Isolation and culture of human pulmonary artery smooth muscle cells (PASMCs)1. The human pulmonary arteries were opened to expose the endothelial surface, which was removed by gentle scraping with a scalpel. 2. The surrounding adventitia was then carefully dissected from the tunica media. 3. Cells were derived either from explants or after tissue digestion. 4. For the explant technique, the pulm......阅读全文
Barretts－esophageal－epithelial－and－fibroblast－primary－cultures
1. Biopsy specimens for tissue culture were immediately placed on ice in primary cell culture system. 2. Within 4 hours from the time of the biopsy, t
Isolation－of－normal－mammary－epithelial－cells
1. A small piece of tissue from each specimen was removed and minced. 2. The tissue was digested with collagenase overnight. 3. To remove the collagen
Isolation－and－cultivation－of－endothelial－progenitor－cells－(EPCs)
Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contri
Isolation,－Culture,－Characterization－of－Cortical－and－Hippocampal－Neurons
实验概要The ability to culture primary neurons under serum-free conditions facilitates tighter control of neuronal studies. Some serum-free media and s
Trabecular－cell－monolayer－culture
Originally described in 1979 and more recently modified by Stamer et al primary trabecular monolayer cell culture has been a cornerstone for inves
Isolation－of－osteoblasts－from－human－bone.
Bone samples were cleaned of adherent soft tissue and osteoblasts isolated by two methods.a. The bone was cut into small pieces (2 mm × 2 mm), washed
Eccles:Protein－Lysates－from－Cells－in－Culture
Cell Lysis Buffer5mL 0.1M Tris HCl pH 8 (10mM)0.44g NaCl (150mM)0.02g EDTA (1mM)0.5mL nonidet P40 (1% w/v)0.05g SDS (0.1% w/v)Make up to 50mL with MQH
Tissue－Culture－of－PtK1－cells
Please note: lately (2005 - present), we normally culture PtK1 cells in F-12 media, but they also grow in other types of media as described below.Mixi
使用CCCadvanced™FN1无异源耗材培养人多能干细胞（四）
Flow cytometry analysis of the quantitative expression of 3 key pluripotency-associated transcription factors (Nanog, OCT3/4 and SOX2) complemen
Human－Embryonic－Stem－(ES)－Cell－Protocols——Freezing－Human－ES－Cells
Collagenase cells for approximately 7 minutes at 37 °C (until edges of colonies are curling up).With a 5 ml pipet, gently pipet and scrape colonies f
Human－Embryonic－Stem－(ES)－Cell－Protocols——Thawing－Human－ES－cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form.Thaw cryovial by gently swirling in waterbath until only a small
Organotypic－raft－culture－of－primary－human－keratinocytes－(PHKs)
Organotypic raft culture of primary human keratinocytes (PHKs)NotesUse 106 keratinocytes per raft.Typical ProtocolTrypsinize the PHKs off the flask an
Stem－cell－characteristics－of－amniotic－epithelial－(AE)－cells
Isolation of AE Cells1. Human placentae were obtained with the approval of the institutional review board, after uncomplicated elective caesarean
Isolation－of－peripheral－blood－mononuclear－cells－(PBMC)
1. Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2. PBMC were isolated by centrifugation over lymphocyte separation medium. 3
RNA－Isolation－From－Animal－tissue－or－cell－culture
实验概要This method is designed for most animal tissues and culture cells. For RNA isolation from fibrous tissue, follow the specialized protocol on pag
细胞组织消化常用的几种酶的选择
直接从生物体获取的组织，一般需要将其消化成单个细胞才能进行体外培养。这种直接从离体组织获得的细胞，更接近于生物体内的生活状态，且生物性状尚未发生很大改变，因此在药物筛选、细胞移植、类器官培养、肿瘤研究等众多领域备受欢迎。但组织消化过程中常遇到多种问题，例如消化不完全、细胞死亡率高等。如何克服这些问题
Human－Embryonic－Stem－(ES)－Cell－Protocols——Splitting－Human－ES－cells－on－MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 °C in a water bath.Aspirate media off of cell culture plate.Add the following a
Human－Embryonic－Stem－(ES)－Cell－Protocols—Splitting－Human－ES－cells－onMatrige
based on splitting onto on plateWarm collagenase media to 37°C in a water bath.Aspirate media off of cell culture plate.Add the following amount of co
兔膀胱平滑肌细胞培养
实验方法原理 运用显微手术器械在肉眼下仔细剔除膀胱黏膜层及浆膜层后，完整分离膀胱平滑肌层。然后以酶分离法获取兔膀胱平滑肌细胞，体外原代和传代培养分离细胞。在倒置显微镜下观察细胞形态及生长状况，同时进行细胞爬片H-E染色和透射电镜等形态学观察分析，并应用免疫荧光染色平滑肌特有的α-肌动蛋白进行细胞学鉴
Isolating－Xenograft－Tumor－Cells－for－Tissue－Culture－or－Transplantation
Isolation of T4 cells from xenograft tumors Put mouse downWash skin by dipping mice into ETOHRemove tumor and put into 5ml PBS containing Fungizone an
Xenofree－Culture－of－Neural－Stem－Cells
实验概要Neural stem cells (NSCs) derived from human embryonic stem cells (hESCs) have the potential to help provide understanding for human neurogenesis
Noninvasive－Human－Nuclear－Transfer－with－Embryonic－Stem－Cells
Noninvasive Human Nuclear Transfer with Embryonic Stem CellsSohyun L. McElroy1 and Renee A. Reijo PeraCenter for Human Embryonic Stem Cell Research an
Peripheral－blood－“endothelial－progenitor－cells”
EPC Isolation and Characterization1. EPCs were obtained by isolating mononuclear cells using Ficoll density-gradient centrifugation of human blood buf
Isolation－and－Transcription－Profiling－of－Purified－Uncultured－Human－Strom...
Isolation and Transcription Profiling of Purified Uncultured Human Stromal Stem CellsIsolation and Transcription Profiling of Purified Uncultured Huma
ELECTROPORATION－OF－ES－CELLS－AND－ISOLATION－OF－H/R－CLONES
Need 1.5-2 x 107 cells from a 2 day culture.1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in cold
Isolation－of－rat－cardiac－fibroblasts－and－cardiomyocytes
1. Hearts were removed from newborn rats (day 0), put into calcium- and bicarbonate-free HEPES-buffered Hanks’ medium, cut into pieces and digeste
Isolation－of－DL(Low－Density－Lipoprotein)－From－Human－Plasma
This procedure involves use of human plasma, a potentially dangerous source of blood-borne disease. Wear long-cuffed gloves, and eye-protection.Day on
兔膀胱平滑肌细胞培养实验——酶分离法
兔膀胱平滑肌细胞培养可以：（1）分离得到高纯度兔膀胱平滑肌细胞；（2）进行细胞学鉴定研究；（3）用于细胞学其他研究。实验方法原理运用显微手术器械在肉眼下仔细剔除膀胱黏膜层及浆膜层后，完整分离膀胱平滑肌层。然后以酶分离法获取兔膀胱平滑肌细胞，体外原代和传代培养分离细胞。在倒置显微镜下观察细胞形态及生长
兔膀胱平滑肌细胞培养实验
酶分离法 实验方法原理 运用显微手术器械在肉眼下仔细剔除膀胱黏膜层及浆膜层后，完整分离膀胱平滑肌层。然后以酶分离法获取兔膀胱平滑肌细胞，体外原代和传代培养分离
兔膀胱平滑肌细胞培养实验
酶分离法 实验方法原理 运用显微手术器械在肉眼下仔细剔除膀胱黏膜层及浆膜层后，完整分离膀胱平滑肌层。然后以酶分离法获取兔膀胱平滑肌细胞，体外原代和传代培养分离